Electrochemical nanobiosensor for real-time detection of gap junction-mediated intercellular communication activity

Grushevskaya, H. V.; Крылова, Н. Г.; Lipnevich, Igor Vladimirovich; Orekhovskaja, T. I.; Shulitski, B. G.

doi:10.5185/amlett.2017.7092

Cited by 6 publications

(5 citation statements)

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“…The complexes Fe(II)DTP 3 of high-spin Fe(II) with thiophene-pyrrole ligands were synthesized by LB-technique at compression of H-dithionilepyrrole molecules on the surface of subphase with salts of three valence Fe. 12 Horizontally and vertically arranged LB-MWCNT-bundles can be fabricated from the multi-walled CNTs. 14 We use the Langmuir-Blodgett technique to fabricate new biocompatible layered nanoheterostructures consisting of two MWCNT LB-monolayers and/or 5-, 7-monolayer LB-film of the organometallic complexes.…”

Section: Methodsmentioning

confidence: 99%

Enhancement of Raman light scattering in dye-labeled cell membrane on metal-containing conducting polymer film

Grushevskaya

Крылова

Lipnevich

et al. 2016

Int. J. Mod. Phys. B

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An enhanced Raman spectroscopy method based on a plasmon resonance in ultrathin metal-containing LB-film deposited on nanoporous anodic alumina supports has been proposed. This material has been utilized to enhance Raman scattering of light in fluorescent-labeled subcellular membrane structures. It has been shown that the plasmon resonance between vibrational modes of the organometallic complexes monolayers and dye-labeled subcellular structures happens. It makes possible to detect interactions between living cell monolayers and an extracellular matrix.

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Section: Methodsmentioning

confidence: 99%

Enhancement of Raman light scattering in dye-labeled cell membrane on metal-containing conducting polymer film

Grushevskaya

Крылова

Lipnevich

et al. 2016

Int. J. Mod. Phys. B

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“…The Bode peak intensities vary weakly for the temperatures of 30,32,34,36,38,40, 50 • C and largely enough for 60, 65, 70 • C in comparison with the intensity of the peak at RT (see Fig. 4).…”

Section: Dielectric Spectral Analysis Of Low-temperature Dna Hybridiz...mentioning

confidence: 87%

“…The electromagnetic field excites the graphenecharge-density oscillations. Resonating with dipole moments of the hydrated complexes of the near electrode double charged layer, the graphene plasmon oscillations decay the hydrated complexes in the Helmholtz double layer and, correspondingly, screen effectively the electrodes [38].…”

Section: B Principle Of Operation Of Capacitive Transducermentioning

confidence: 99%

High sensitivity electrochemical DNA sensors for detection of somatic mutations in FFPE samples

Egorova¹,

Grushevskaya²,

Крылова³

et al. 2022

Preprint

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We offer new high-performance label-free electrochemical impedimetric DNA sensors of nonfaradaic type. The DNA sensors based on a platform of crystalline carbon nanotube (CNT) arrays are fabricated by the Langmuir-Blodgett (LB) deposition technique. The CNT arrays are suspended on a nanoporous anodic alumina (aluminium oxide, AOA) support. Single-stranded (ss) 19-and 20-base oligonucleotides and double-stranded (ds) nucleotide sequences were used as probes for chosen molecular target -human KRAS (Kirsten Rat Sarcoma viral oncogene homolog) gene. Genomic deoxyribonucleic acids (DNAs) were isolated from placenta of healthy donors and FFPE (formalin-fixed, paraffinembedded) samples of tumor tissue. Raman spectral analysis and electrochemical dielectric spectroscopy were used to sequence the target DNAs. The optical and electrochemical detection (variants of DNA sequencing) were based on a screening effect that grows after the DNA homoduplex formation. We demonstrated that such technologies are very sensitive and allow to detect attomolar DNA concentrations and less. As result, the KRAS exon 2, codon 12, c.35G>A mutation was successfully discriminated in human genomic DNA isolated from FFPE colorectal cancer tumor tissue samples.

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“…An amphiphilic oligomer of thiophenepyrrole derivatives with chemically bounded hydrophobic 16-link hydrocarbon chain R: 3hexadecyl-2,5-di(2-thienyl)-1H-pyrrole (H-DTP, H-dithienylpyrrole), chemical structure of which is shown in Fig. 1a was utilized to synthesize a nanocyclic compound (Fe(II)DTP) of the dithienylpyrrole with hight-spin divalent Fe(II) decorating carbon nanotubes [17]. The Fe(II)DTP films are fabricated by the LB technique [18].…”

Section: Methodsmentioning

confidence: 99%

“…2). The Raman metallic-SWCNT bands are exhibited among with the Raman bands of the Fe(II)DTP [17]. The environmental coefficient C env for the CNT bilayer decreases relative to its value for the inverse micelles with the metallic SWCNTs from 0.0312 to 0.0303 due to the lack of interactions in the direction orthogonal to the CNT axis.…”

Section: Physicochemical Characterization Of Carboxylated Carbon Nano...mentioning

confidence: 95%

Topologically Tuned Obliquity of Klein-Tunnelling Charged Currents Through Graphene Electrostatically-Confined p - n Junctions

Grushevskaya

Krylov

2022

Nonlinear Phenomena in Complex Systems

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Problem of control over Klein-tunnelling states from electrostatically-confined graphene p - n junctions has been discussed. The lack of quasi-bound states, being the states with a finite life time, in a pseudo-Dirac-fermion model for the graphene quantum dot (GQD) is theoretically predicted as inapplicability of the so-called "resonance condition" leading to an inconsistent linear system corresponding to matching conditions. Within a pseudo-Dirac-Weyl fermion model GQD, the graphene charge carriers are topologically nontrivial and can be confined by a staircase-type potential due to competition between Zak curvature and centrifugal-force actions. The predicted topological effects elucidate experimentally observed resonances created by electron beam and laser pulse in crystalline arrays of single-walled carbon nanotubes as the Klein-tunnelling resonant states in the p - n graphene junctions. We present a robust approach to fabricate stable graphene p - n junctions by fine-tuning the topological effects.

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Electrochemical nanobiosensor for real-time detection of gap junction-mediated intercellular communication activity

Cited by 6 publications

References 0 publications

Enhancement of Raman light scattering in dye-labeled cell membrane on metal-containing conducting polymer film

Enhancement of Raman light scattering in dye-labeled cell membrane on metal-containing conducting polymer film

High sensitivity electrochemical DNA sensors for detection of somatic mutations in FFPE samples

Topologically Tuned Obliquity of Klein-Tunnelling Charged Currents Through Graphene Electrostatically-Confined p - n Junctions

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