Electrochemical processing of fibrinogen modified-graphite surfaces: Effect on plasmin generation from adsorbed plasminogen

Alfonsi-Hourdin, Séverine; Longchamp, Sylvie; Gallet, Olivier; Nigretto, Jean-Maxime

doi:10.1016/j.biomaterials.2005.05.092

Cited by 3 publications

(2 citation statements)

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“…Adsorption using low concentration of protein solution, may also favor spreading and denaturation of adsorbed protein, because adsorption occurs slowly and proteins have time to unfold before the entire surface is covered [16,20]. This is associated with the accessibility sites of important domain in the fibrinogen structure that could be or not present to interact with other proteins or cells [21].…”

Section: Article In Pressmentioning

confidence: 99%

Fibrinogen adsorption, platelet adhesion and activation on mixed hydroxyl-/methyl-terminated self-assembled monolayers

et al. 2006

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Section: Article In Pressmentioning

confidence: 99%

Fibrinogen adsorption, platelet adhesion and activation on mixed hydroxyl-/methyl-terminated self-assembled monolayers

et al. 2006

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“…Various strategies have been used to control surface coagulation including coating the surface with protein-resistant polymers, − active molecules such as anticoagulants , or fibrinolysis promoters, − endothelial cells , or polymers mimicking their membrane, , and last but not least inorganic thin films conferring various surface properties as reviewed by Mani and colleagues . A strategy not comprised in this list was proposed by P. N. Sawyer more than 40 years ago and consisted in cathodically polarizing the implant’s surface.…”

Section: Introductionmentioning

confidence: 99%

Coagulation at the Blood–Electrode Interface: The Role of Electrochemical Desorption and Degradation of Fibrinogen

et al. 2014

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The influence of electrochemistry on the coagulation of blood on metal surfaces was demonstrated several decades ago. In particular, the application of cathodic currents resulted in reduced surface thrombogenicity, but no molecular mechanism has been so far proposed to explain this observation. In this article we used for the first time the quartz crystal microbalance with dissipation monitoring technique coupled with an electrochemical setup (EQCM-D) to study thrombosis at the blood-electrode interface. We confirmed the reduced thrombus deposition at the cathode, and we subsequently studied the effect of cathodic currents on adsorbed fibrinogen (Fg). Using EQCM and mass spectrometry, we found that upon applying currents Fg desorbed from the electrode and was electrochemically degraded. In particular, we show that the flexible N-terminus of the -chain, containing an important polymerization site, was cleaved from the protein, thus affecting its clottability. Our work proposes a molecular mechanism that at least partially explains how cathodic currents reduce thrombosis at the blood-electrode interface and is a relevant contribution to the rational development of medical devices with reduced thrombus formation on their surface. ABSTRACTThe influence of electrochemistry on the coagulation of blood on metal surfaces was demonstrated several decades ago. In particular, the application of cathodic currents resulted in reduced surface thrombogenicity, but no molecular mechanism has been so far proposed to explain this observation. In this article we used for the first time the quartz crystal microbalance with dissipation monitoring technique coupled with an electrochemical setup (EQCM-D) to study thrombosis at the blood-electrode interface. We confirmed the reduced thrombus deposition at the cathode, and we subsequently studied the effect of cathodic currents on adsorbed fibrinogen (Fg). Using EQCM and mass spectrometry, we found that upon applying currents Fg desorbed from the electrode and was electrochemically degraded. In particular, we show that the flexible N-terminus of the α-chain, containing an important polymerization site, was cleaved from the protein, thus affecting its clottability. Our work proposes a molecular mechanism that at least partially explains how cathodic currents reduce thrombosis at the blood-electrode interface and is a relevant contribution to the rational development of medical devices with reduced thrombus formation on their surface.2

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Fibrinogen‐Coated Bismuth Film Electrodes for Voltammetric Analysis of Lead and Cadmium using the Batch Injection Analysis

Adraoui

Rhazi²,

Amine

2007

Analytical Letters

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Electrochemical processing of fibrinogen modified-graphite surfaces: Effect on plasmin generation from adsorbed plasminogen

Cited by 3 publications

References 42 publications

Fibrinogen adsorption, platelet adhesion and activation on mixed hydroxyl-/methyl-terminated self-assembled monolayers

Fibrinogen adsorption, platelet adhesion and activation on mixed hydroxyl-/methyl-terminated self-assembled monolayers

Coagulation at the Blood–Electrode Interface: The Role of Electrochemical Desorption and Degradation of Fibrinogen

Fibrinogen‐Coated Bismuth Film Electrodes for Voltammetric Analysis of Lead and Cadmium using the Batch Injection Analysis

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