Electrochemical Sensing Based on Nitrogen-Doped Hollow Carbon Nanospheres Wrapped with MoS 2 Nanosheets

In this work, the development of riboflavine sodium phosphate (RFSP) decorated WS 2 nanosheets and their usability for electrochemical monitoring of hybridization of nucleic acids have been achieved. The RFSP molecules performed as highly efficient stabilizer for ultrasonically dispersing WS 2 nanosheets in an aqueous solution, as well as providing the inherent self-redox signal for sensing DNA immobilization and hybridization. The probe ssDNA was noncovalently assembled on the fabricated RFSP/WS 2 nanocomposite surface through the π-π interaction between the conjugated nanocomposite and nucleic acids bases. The formed dsDNA after the hybridization of the probe with the target could be released off the surface of the conjugated nanocomposite because of the burying of DNA bases. This constructed genosensor permits quantitative determination of PIK3CA gene in the range of 1.0 × 10 −16 to 1.0 × 10 −8 mol l −1 with a detection limit as low as 2.1 × 10 −17 mol l −1 . The proposed platform exhibited a desirable differentiation capability against complementary, noncomplementary and base-mismatched sequences along with favorable stability and reproducibility. This approach of genosensing can provide a facile and rapid mode for DNA determination without the utilization of additional labels.

mentioning

confidence: 97%

Self-Signal Electrochemical Monitoring of Hybridization of Nucleic Acids Based on Riboflavine Sodium Phosphate Decorated WS₂ Nanosheets

Yang

Zhang

2020

“…However, TMDs still face great challenges as electrode materials, and their practical applications are limited by defects such as unavoidable material stacking and low electronic conductivity. 29,30 In addition, there is no clear explanation for the catalytic mechanism of two-dimensional sulfide materials in DA detection, which limits its scope of application of layered sulfide materials in this field.…”

mentioning

confidence: 99%

Electrochemical Sensor Based on Co-Doped FePS₃ Nanosheets for Ultra-Sensitive Detection of Dopamine in Human Serum

Shan

Chen

et al. 2023

Electrochemical dopamine (DA) sensors become important for early diagnosis of psychiatric disorders like schizophrenia and Parkinson's disease due to their fast response, simplicity, and portability. However, traditional electrode modification materials such as noble metals and metal oxides have shortcomings such as high cost, low conductivity, or limited catalytic performance. Two-dimensional sulfide materials contribute to the smooth electrode reaction because of their ultra-high specific superficial area and favorable electrocatalysis properties, however, their low carrier mobility and poor electroconductibility limit the detection signal. In this paper, Co-doped FePS3 nanosheets were employed for DA detection for the first time. Fe0.9Co0.1PS3 nanosheets exhibited a detection limit of 120 nM, a linear range 0.25-100 μM and 120-500 μM, and possessed high recovery and reproducible stability when applied to human serum samples. Furthermore, according to the in-situ XPS characterization, S atoms located on the outmost layer of Fe0.9Co0.1PS3 nanosheets could be combined with the phenolic hydroxyl oxygen of DA, which makes electrode reaction from DA to dopamine quinone easier. Co-doping can further enhance the above effect, and increase the carrier mobility of FePS3 nanosheets. This work demonstrates that electrochemical sensors based on metal phosphorus trisulfide materials have tremendous potential for future application in mental disorder diagnosis

“…Interestingly, the MoS 2 nanosheets with large surface area can be combined with other materials to develop novel nanocomposites in the electrochemical sensing field. [18][19][20][21][22][23] For instance, Sun et al fabricated a three-dimensional electrochemical sensor with dual signal amplification based on MoS 2 nanosheets and high-conductive amino-functionalized carbon nanotubes @ covalent organic frameworks (NH 2 -MWCNT@COF) for sulfamerazine determination. 18 Yuan et al reported an aptamer based voltammetric biosensor for endotoxins using a functionalized graphene and MoS 2 nanosheets composite as a new nanocarrier.…”

mentioning

confidence: 99%

Surface-Functionalized MoS₂ Nanosheets Sensor for Direct Electrochemical Detection of PIK3CA Gene Related to Lung Cancer

Zhang

Yang

2020

An electrochemical biosensor for direct detection of PIK3CA gene related to lung cancer was developed based on riboflavin 5′monophosphate sodium salt (FMNs)-functionalized MoS 2 nanosheets prepared by simple ultrasonication method. As a highly effective biodispersant, FMNs demonstrated excellent stabilization for obtaining highly dispersed MoS 2 nanosheets in an aqueous solution. The prepared FMNs/MoS 2 nanocomposite with fine electrochemical redox activity was employed as the platform to immobilize DNA probe and supplied the inherent indicator signal for DNA sensing. The amine-terminated ssDNA probe was covalently immobilized onto the FMNs/MoS 2 nanocomposite with rich phosphonate groups. After the hybridization of the probe ssDNA with the complementary target DNA, the self-redox signal of the nanocomposite decreased ascribed to the changes in the conformation of DNA. The results indicated that the proposed self-signal detection platform displayed high sensitivity with a detection limit of 1.2 × 10 -17 mol l −1 for PIK3CA gene from lung cancer, accompanied with fine selectivity, outstanding stability and reproducibility. The constructed biosensor was easy to design and had the potential for the detection of different pathogenic DNA without intricate label process.