Electrochemical studies on the interaction of heparin with crystal violet and its analytical application

Sun, Wei; Ding, Ya-Qin; Jiao, Kui

doi:10.1134/s1061934806040113

Cited by 20 publications

(18 citation statements)

References 12 publications

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“…40 Thus, the ratio between absorption peaks at 490 and 590 nm (490/590 absorption ratio) was used to measure the binding between CV and heparin (Figure 2). …”

Section: Resultsmentioning

confidence: 99%

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Heparin-based hydrogels with tunable sulfation & degradation for anti-inflammatory small molecule delivery

2016

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Sustained release of anti-inflammatory agents remains challenging for small molecule drugs due to their low molecular weight and hydrophobicity. Therefore, the goal of this study was to control the release of a small molecule anti-inflammatory agent, crystal violet (CV), from hydrogels fabricated with heparin, a highly sulfated glycosaminoglycan capable of binding positively-charged molecules such as CV. In this system, both electrostatic interactions between heparin and CV and hydrogel degradation were tuned simultaneously by varying the level of heparin sulfation and varying the amount of dithiothreitol within hydrogels, respectively. It was found that heparin sulfation significantly affected CV release, whereby more sulfated heparin hydrogels (Hep and Hep−N) released CV with near zero-order release kinetics (R-squared values between 0.96-0.99). Furthermore, CV was released more quickly from fast-degrading hydrogels than slow-degrading hydrogels, providing a method to tune total CV release between 5-15 days while maintaining linear release kinetics. In particular, N-desulfated heparin hydrogels exhibited efficient CV loading (~90% of originally included CV), near zero-order CV release kinetics, and maintenance of CV bioactivity after release, making this hydrogel formulation a promising CV delivery vehicle for a wide range of inflammatory diseases.

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“…40 Thus, the ratio between absorption peaks at 490 and 590 nm (490/590 absorption ratio) was used to measure the binding between CV and heparin (Figure 2). …”

Section: Resultsmentioning

confidence: 99%

“…The absorbance was then assessed at 490 and 590 nm using a UV-vis spectrometer (SpectraMax M2, Molecular Device), where absorbance at 490 nm was characteristic of CV bound to heparin and absorbance at 590 nm was characteristic of unbound CV (n = 3). 40 …”

Section: Methodsmentioning

confidence: 99%

Heparin-based hydrogels with tunable sulfation & degradation for anti-inflammatory small molecule delivery

2016

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“…A number of reports are available for the determination of Levofloxacin using different anionic dyes using spectrophotometric techniques at acidic pH [4][5][6][7][8]. Different techniques have been reported for the determination of Levofloxacin by Spectrophotometry [9][10][11], High Performance liquid chromatography [12], Capillary zone electrophoresis [13], Ligand exchange chromatography [14] and Nuclear magnetic resonance spectroscopy [15].…”

Section: Structure Of Levofloxacinmentioning

confidence: 99%

A Green Electroanalytical Method for the Determination of Levofloxacin by Ion-Pair Formation with Picric Acid

Mittal¹

2016

JAPLR

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Levofloxacin (LFX), an antimicrobial agent has been detected by using picric acid (PA) as an anionic reagent in aqueous medium. On mixing the reactants in distilled water yellow colored product is separated within two minutes which was found to be an ionassociation complex (LFX-PA) of both the reactants. Spectroscopic and voltammetric studies were carried out to explain the interaction between levofloxacin and picric acid. Spectroscopic study of the ion-pair product confirmed its formation as determined from substantial shift in respective λ max values. In voltammetric experiments, a shift of 70 mV was noticed in an anodic peak of LFX-PA (1.64V) accompanied with a large decrease in peak current. In addition, a number of new peaks appeared in voltammogram of LFX-PA. Similarly, in case of cathodic cycle, new peaks appeared in differential pulse voltammogram of the ion-pair which proved the interaction of the PA and LFX. A calibration curve was drawn using the peak current values of the LFX. The linear increase in peak current with increasing LFX concentration was obtained in the concentration range 1.5×10-3 M -6.0×10 -3 M with R 2 = 0.994. A study on the solvent effect on the spectrometric as well as voltammetric methods for LFX suggested that water is the best medium for the study. Stability of the complex was also determined by computational methods using DFT/B3LYP/6-31G basis sets. Further, analysis of LFX in commercially available drug tablets proved that the proposed method is valid in real life sample analysis.

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“…13 Sun et al applied some organic dyes as the electrochemical probes for the determination of protein 14,15 and heparin. 16,17 Lu et al also studied the interaction of bovine serum albumin with acid cyanine 5R and applied to the protein analysis. 18 Lysozyme (LYS) is an enzyme commonly present in egg white, tears and other secretions.…”

Section: Introductionmentioning

confidence: 98%

Linear sweep voltammetric studies on the supramolecular complex of alizarin red S with lysozyme and determination of lysozyme

et al. 2009

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An electrochemical method for the determination of lysozyme (LYS) based on its interaction with alizarin red S (ARS) was established by linear sweep voltammetry in this paper. The electrochemical behaviour of ARS with LYS was investigated on a dropping mercury working electrode in 0⋅2 mol/L pH 4⋅8 Britton-Robinson (B-R) buffer solution. ARS showed a sensitive second order derivative linear sweep voltammetric reductive peak at -0⋅42 V (vs SCE). After the addition of LYS, the reductive peak current of ARS decreased without the shift of the reductive peak potential and no new waves appeared, which was due to the formation of a supramolecular complex of ARS with LYS in the solution. The stoichiometry of the ARS-LYS complex was further calculated by the electrochemical data with the results of the binding ratio as 3 : 1 and the binding constant as 2⋅82 × 10 14 . Under the selected conditions, the decrease of the second order derivative linear sweep voltammetric reductive peak current of ARS was in proportion to the LYS concentration in the range from 0⋅8 to 35⋅0 mg/L and the detection limit of LYS was calculated as 0⋅52 mg/L (3σ). Different kinds of LYS samples were detected satisfactorily with this method.

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Electrochemical studies on the interaction of heparin with crystal violet and its analytical application

Cited by 20 publications

References 12 publications

Heparin-based hydrogels with tunable sulfation & degradation for anti-inflammatory small molecule delivery

Heparin-based hydrogels with tunable sulfation & degradation for anti-inflammatory small molecule delivery

A Green Electroanalytical Method for the Determination of Levofloxacin by Ion-Pair Formation with Picric Acid

Linear sweep voltammetric studies on the supramolecular complex of alizarin red S with lysozyme and determination of lysozyme

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