Electrochemiluminescence resonance energy transfer biosensing platform between g-C3N4 nanosheet and Ru–SiO2@FA for dual-wavelength ratiometric detection of SARS-CoV-2 RdRp gene

High-Density N-Vacancy-Induced Multipath Electrochemiluminescence Improvement of 3D g-C3N4 for Ultrasensitive MiRNA-222 Analysis

“…Electrochemiluminescence (ECL) was considered as a promising tool in analytical fields due to its high sensitivity and simple and controllable operation. − Among various ECL emitters, g-C 3 N 4 was widely used due to its nontoxicity and low cost. − To enhance the ECL intensity, S 2 O 8 2– was often used as its cathodic co-reactant. − However, excessive S 2 O 8 2– with strong oxidation may cause oxidative damage to biomolecules and interference with biological samples. In previous work, we found that the endogenic dissolved O 2 could act as a cathodic co-reactant of three-dimensional (3D) g-C 3 N 4 to enhance its ECL emission, which could solve the above problem, and had further advantages of good biocompatibility and simple operation .…”

Biosensing strategies for the detection of SARS-CoV-2 nucleic acids

“…The biorecognition layer was built at a gold electrode and consisted of a rigid DNA tetrahedron which contains the probe in the vertex and this scaffold makes posible that the target-participated entropy-driven amplified reaction occurs and efficiently propiciate the link of the [Ru(bpy) 3 ] 2+ modified DNA to the linear ssDNA at the vertex of the tetrahedron to give the ECL. Yin et al [38] proposed a dual wavelength ratiometric ECL genosensor for the ultrasensitive detection of the SARS-CoV-2 RdRp gene based on resonance energy transfer (RET) between graphitic carbon nitride nanosheets (g-C 3 N 4 ) and Ru-SiO 2 nanospheres functionalized with folic acid (Ru-SiO 2 @FA), using a bipedal three-dimensional (3D) DNA walker amplification strategy ( Fig. 6 ).…”

A multifunctional electrochemiluminescence and photoelectrochemical biosensor based on a quantum dot ion-exchange reaction for two-channel detection of thrombin

“…[13][14][15] Compared to photoluminescence or other optical methods that rely on external light illumination, it has better space-time control and low cost. [16][17][18] In PEC methods, the excitation source is provided by external light and the output signal is photocurrent. [19][20][21] Consequently, the interference from the excitation light source is prevented.…”