Electrochemotherapy can eradicate established colorectal carcinoma and leaves a systemic protective memory in mice.

Electrochemotherapy induces apoptotic death in melanoma metastases: a histologic and immunohistochemical investigation

“…Tumor development from cancer cell reinjection was hampered in survived animals. Thus, ECT seems to confer protective immunity toward experimental tumor re-challenge 33. These results were confirmed in melanoma-bearing mice that showed protection toward tumor reinjection after ECT treatment.…”

Possible Involvement of Antitumor Immunity in the Eradication of Colon 26 Induced by Low-Voltage Electrochemotherapy with Bleomycin

“…6,21,22 The induction of cytotoxic T lymphocytes against Colon 26 cells was also observed in the spleens of mice with Colon 26 after treatment with high-voltage electrochemotherapy and bleomycin. 8 We focused on this topic and found that Balb/c mice had protective tumor immunity induced by low-voltage electrochemotherapy with bleomycin, but Balb/c nu/nu nude mice failed to eradicate Colon 26 tumors by means of electrochemotherapy. Therefore, electrochemotherapy may increase an immune response to tumors, which in turn may be exploited with immunotherapy, in order to increase response rates.…”

“…This result has contributed to improved drug penetration into tumor cells. 4,5 Electrochemotherapy has been found to increase immune response to tumors as well [6][7][8] and the infiltration of inflammatory cells into tumor nodules treated with bleomycin and electric pulse was also reported. 9 Based on this phenomenon, it is possible that a host-immune mechanism is involved in tumor regression after electrochemotherapy; however, its precise nature has yet to be defined.…”

A Potential Approach for Electrochemotherapy against Colorectal Carcinoma Using a Clinically Available Alternating Current System with Bipolar Snare in a Mouse Model

“…Electrochemotherapy is considered to be theoretically effective for the treatment of any histological types of cancer if electrodes for delivering electric pulses are accessible to tumors. We previously showed that electrochemotherapy with BLM has a great impact on survival of mice with CRC not only in an ectopic model but also in an orthotopic model (10)(11)(12)(13)(14). So far all in vivo studies including not only animal models but also clinical trials have used direct current, square wave electric pulses.…”

“…Electrochemotherapy is supposed to be theoretically effective for the treatment of any histological types of cancer. We showed previously that electroche-ORIGINAL ARTICLE Ó 2001 Taylor & Francis motherapy with bleomycin (BLM), 5-uorouracil (5-FU) or cis-diamminedichloroplatinum(II) (CDDP) could induce signi cantly prolonged survival of mice with CRC compared with the treatment with the drug alone not only in an ectopic model but also in an orthotopic model (10)(11)(12)(13)(14). Although 5-FU and CDDP have been the most frequently used chemotherapeutic agents for the clinical treatment of CRC, the most successful drug in electrochemotherapy against CRC was BLM (11,12).…”