Electrochromatographic studies on infectious nucleic acid from tobacco mosaic virus

Takahashi, William N.; Karler, Arthur; Knight, C.A.

doi:10.1016/0042-6822(58)90112-0

Cited by 13 publications

(1 citation statement)

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“…The term "viral RNA" is here used to designate the ribonuclease-sensitive infective fraction of phenol-extracted virus. It is not germane to the present consideration whether some amount of viral protein must remain associated with ribonucleic acid to retain infectivity (14). Whether phenol-extracted virus yielded ribonucleic acid or ribonucleoprotein, the RNA material bypassed an initial stage of infection which limited host range of intact poliovirus to primate cells.…”

Section: Discussionmentioning

confidence: 99%

The Mammalian Cell Virus Relationship

Holland

McLaren

Syverton

1959

The Journal of Experimental Medicine

201

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Primate cells naturally susceptible to poliovims infection possess specific surface receptors that permit poliovirus to be adsorbed quantitatively, received within the cell, eclipsed with loss of infectivity, replicated, and released with accompanying cytopathic effect. Conversely, non-primate cells are naturally insusceptible to poliovirus infection, do not possess active receptors, adsorb only a small fraction of supplied poliovirus, fail to eclipse or replicate measurable amounts of the cell-associated virus, and undergo no cytopathic change (1, 2). It was of interest to learn whether the specific capacity of primate ceils to receive, replicate, and release poliovirus was conditioned wholly by presence of specific receptors. Preliminary findings indicated lack of receptors required for cell infection by poliovirus could be surmounted by use of viral RNA (3).The present communication describes production of infectious poliovirus and other enteroviruses by non-primate cells exposed to viral ribonucleic acid, comparative efficiency of non-primate and primate cells for such virus production, and properties of the produced virus. Materials and MethodsCell Culture Methods.--Cell sources, cultural methods, solutions, and media have been described previously (I, 4).

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Section: Discussionmentioning

confidence: 99%