Electroejaculation in the Chinchilla (Chinchilla lanigera): effects of anesthesia on seminal characteristics

Busso, Juan Manuel; Ponzio, Marina Flavia; Chiaraviglio, Margarita; Cúneo, Marta Fiol de; Ruiz, Rubén Daniel

doi:10.1016/j.rvsc.2004.06.004

Cited by 36 publications

(37 citation statements)

References 15 publications

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“…Esse resultado é mais elevado que o encontrado por Morato et al (1998), os quais observaram 3,7% de amostras seminais contaminadas com urina em onças (Panthera onca), após uso da eletroejaculação. No entanto, Busso et al (2004), ao utilizarem a técnica em chinchilas (Chinchilla lanigera), obtiveram amostras seminais totalmente livres de urina em animais anestesiados. A causa da contaminação por urina pode estar relacionada ao posicionamento mais cranial do eletrodo, já que a inervação controladora da micção está muito próxima à inervação controladora da ejaculação, e o aprofundamento do eletrodo no momento da ejaculação pode ocasionar a micção (Martin 1978).…”

Section: Eficiência Da Eletroejaculaçãounclassified

Biometria testicular, eletroejaculação e características seminais de caititus, Tayassu tajacu Linnaeus, 1758 (Mammalia, Artiodactyla, Tayassuidae) mantidos em cativeiro na Amazônia Oriental

et al. 2010

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Estudos relacionados à obtenção e avaliação de sêmen de Tayassu tajacu são escassos, sendo necessárias pesquisas a respeito. Os objetivos do estudo foram avaliar a biometria testicular de caititus adultos cativos, testar a eficiência da eletroejaculação para obtenção de sêmen e avaliar suas características seminais ao longo do ano. Procedeu-se à eletroejaculação em oito animais adultos e as amostras de sêmen colhidas foram avaliadas quanto às características físicas e morfológicas. Os animais tinham testículo esquerdo com 3,8 ± 0,4 cm X 2,6 ± 0,3 cm e 2,3 ± 0,2 de consistência, e testículo direito com 3,8 ± 0,5 cm X 2,7 ± 0,3 cm e 2,3 ± 0,2 de consistência. A taxa de sucesso nas colheitas foi de 75,21%. O sêmen possuiu: volume 0,81 ± 0,86 mL, concentração 137,44 ± 153 x 106 sptz mL-1, pH 7,92 ± 0,73, motilidade 52,66 ± 28,79%, vigor 2,2 ± 0,8, integridade de membrana plasmática 55,84 ± 28,55%, defeitos maiores 22,87 ± 12,93%, defeitos menores 9,11 ± 5,88% e defeitos totais 31,52 ± 13,81%. Os animais apresentaram simetria testicular, a eletroejaculação se mostrou eficiente para a obtenção de ejaculados em caititus e as flutuações observadas na produção seminal não foram suficientes para caracterizá-los como animais de reprodução sazonal.

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Section: Eficiência Da Eletroejaculaçãounclassified

Biometria testicular, eletroejaculação e características seminais de caititus, Tayassu tajacu Linnaeus, 1758 (Mammalia, Artiodactyla, Tayassuidae) mantidos em cativeiro na Amazônia Oriental

et al. 2010

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“…Assisted breeding techniques such as electroejaculation, artificial insemination, and cryopreservation of spermatozoa have also been extensively studied by researchers at the University of Cordoba in Argentina, who have been facilitating captive breeding programs for this endangered species over the past 10 years (Busso et al, 2005;Busso et al, 2012;Dominchin et al, 2014;Ponzio et al, 2007;Ponzio et al, 2011).…”

Section: Laboratory Animal Medicinementioning

confidence: 99%

Biology and Diseases of Chinchillas

Hsu

Chan²,

Wheler

2015

Laboratory Animal Medicine

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“…Despite growing popularity of long‐tailed chinchillas ( Chinchilla lanigera ) as pets, their wild counterparts are critically endangered (Busso, Ponzio, Chiaraviglio, de Cuneo, & Ruiz, ). Implementation of artificial reproductive techniques (ART), such as artificial insemination and preservation of sperm, would significantly improve the exchange of genetic material between breeders and farms, and can be a valuable tool in the protection programs for free‐living chinchillas.…”

Section: Introductionmentioning

confidence: 99%

“…Most studies about chinchilla semen were conducted on spermatozoa obtained by electroejaculation (Barnabe, Duarte, Barnabe, Visintin, & de Frettas, 1994;Busso et al, 2005;Carrascosa et al, 2001;Niedbała et al, 2015;Ponzio, Busso, de Cuneo, Ruiz, & Ponce, 2008) and only one described spermatozoa collected from epididymis (Ponce et al, 1998). In chinchillas epididymal sperm can be obtained only after testicle removal (after castration or death), which makes this method useless in the case of active stud males.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the characteristics of chinchilla epidydimal semen after collection, storage at 5°C and cryopreservation

Polit

Prochowska

Niżański

2018

Reprod Domestic Animals

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Contents The aim of the study was to compare the characteristics of chinchilla epididymal sperm: fresh, stored at liquid state and cryopreserved. Epididymal spermatozoa obtained from 11 males were assessed for subjective motility, concentration, motility parameters measured by CASA, viability, morphology, membrane integrity, acrosome integrity, mitochondrial potential, lipid peroxidation, chromatin structure, apoptotic changes and capacitation. Then half of the spermatozoa were stored at 5°C for 30 hr, and the second half was cryopreserved. After storage and thawing the same parameters as in fresh semen were assessed. Fresh semen showed good quality, with low levels of lipid peroxidation, chromatin fragmentation and capacitation. CASA evaluation showed significantly lower values for MOT, PMOT, RAPID, VCL, VAP and VSL after both storage at liquid state and cryopreservation (p < 0.05). Cold storage did not induce membrane and acrosome damage (p > 0.05), conversely to cryopreservation (p < 0.05). After storage, there was a drop in high mitochondrial potential in live cells (p < 0.05) and an increase in the percentage of non‐apoptotic, capacitated cells (p < 0.05). These changes were not seen after cryopreservation (p > 0.05). Lipid peroxidation in live cells and chromatin structure remained unchanged both after storage and cryopreservation (p > 0.05). The study showed that examined methods of semen preservation exerted different patterns of changes in spermatozoa and that sperm quality after both of them allowed for further use of preserved spermatozoa in artificial reproductive techniques.

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Electroejaculation in the Chinchilla (Chinchilla lanigera): effects of anesthesia on seminal characteristics

Cited by 36 publications

References 15 publications

Biometria testicular, eletroejaculação e características seminais de caititus, Tayassu tajacu Linnaeus, 1758 (Mammalia, Artiodactyla, Tayassuidae) mantidos em cativeiro na Amazônia Oriental

Biometria testicular, eletroejaculação e características seminais de caititus, Tayassu tajacu Linnaeus, 1758 (Mammalia, Artiodactyla, Tayassuidae) mantidos em cativeiro na Amazônia Oriental

Biology and Diseases of Chinchillas

Comparison of the characteristics of chinchilla epidydimal semen after collection, storage at 5°C and cryopreservation

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