Electrokinetic chromatography with polymeric pseudostationary phases

Palmer, Christopher P.

doi:10.1002/jssc.200700524

Cited by 11 publications

(7 citation statements)

References 80 publications

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“…Several reviews have been dedicated to the use of various chiral surfactants such as molecular micelles [ 18 , 19 , 20 , 21 , 22 ] and glycosidic surfactants [ 23 ]. However, a Scopus search revealed that no reviews were made for bile salts since the review by Otsuka and Terabe two decades ago [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Bile Salts in Chiral Micellar Electrokinetic Chromatography: 2000–2020

Quirino

2021

Molecules

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Bile salts are naturally occurring chiral surfactants that are able to solubilize hydrophobic compounds. Because of this ability, bile salts were exploited as chiral selectors added to the background solution (BGS) in the chiral micellar electrokinetic chromatography (MEKC) of various small molecules. In this review, we aimed to examine the developments in research on chiral MEKC using bile salts as chiral selectors over the past 20 years. The review begins with a discussion of the aggregation of bile salts in chiral recognition and separation, followed by the use of single bile salts and bile salts with other chiral selectors (i.e., cyclodextrins, proteins and single-stranded DNA aptamers). Advanced techniques such as partial-filling MEKC, stacking and single-drop microextraction were considered. Potential applications to real samples, including enantiomeric impurity analysis, were also discussed.

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Section: Introductionmentioning

confidence: 99%

Bile Salts in Chiral Micellar Electrokinetic Chromatography: 2000–2020

Quirino

2021

Molecules

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“…EKC with the use of polymers as a pseudostationary phase with a large variety of chemical structures can be used to improve the selectivity or performance [18].…”

Section: Introductionmentioning

confidence: 99%

Novel and highly sensitive mixed‐polymeric electrokinetic chromatography system for determination of contaminants and impurities of heparin samples

et al. 2010

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A mixed-polymeric electrokinetic chromatography system has been developed for the simultaneous determination of a contaminant like oversulfated condroitin sulfate (OSCS) and impurities expressed as dermatan (Der) in heparin (Hep) samples. The EKC system consisted of 0.5% w/v polymeric β-CD, 0.4% w/v tetronic(®) 1107 and 400 mM tris-phosphate buffer at pH 3.5. The optimized electrophoretic conditions included the use of an uncoated-silica capillary of 50 cm of total length and 75 μm id, an applied voltage of -7 kV, a temperature of 30°C and 200 nm UV-detection. The highly sensitive method developed showed low values of LOD, 0.07% w/w (0.07 μg/mL) (OSCS) and 0.1% w/w (0.1 μg/mL) (Der), and values of LOQ 0.2% w/w (0.2 μg/mL) (OSCS) and 0.3% w/w (0.3 μg/mL) (Der) with a concentration level of Hep sample as low as 0.1 mg/mL. Additional parameters of validation such as specificity, linearity, accuracy, and robustness were evaluated according to international guidelines. Owing to its simplicity, high sensitivity, and reliability, the proposed method can be an advantageous alternative to the traditional methodologies for the analysis of Hep in raw material and specially in finished products because of the low amounts of Hep sample required.

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“…Also included are the developments in the use of polymeric nanoparticles as PSPs since 2004, the last time this was reviewed in this series 14. Additional reviews of the developments in polymeric and nanoparticle PSPs have appeared during the current review period 16–19.…”

Section: Introductionmentioning

confidence: 99%

Recent progress in the use of ionic polymers as pseudostationary phases for electrokinetic chromatography

Palmer

2009

Electrophoresis

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This review is an account of the development, utilization, and characterization of polymeric pseudostationary phases (PSPs) for EKC since the previous review in this series in 2007. Improvements in performance by means of new polymeric phases and modifications to previously reported materials are described. The use of EKC and spectroscopic studies to characterize the interactions between polymeric PSPs and analytes is discussed. The application of polymeric PSPs for techniques or approaches to which they provide particular advantages is reviewed. Chief among these is the application of polymeric PSPs for the combination of EKC with MS detection.

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Electrokinetic chromatography with polymeric pseudostationary phases

Cited by 11 publications

References 80 publications

Bile Salts in Chiral Micellar Electrokinetic Chromatography: 2000–2020

Bile Salts in Chiral Micellar Electrokinetic Chromatography: 2000–2020

Novel and highly sensitive mixed‐polymeric electrokinetic chromatography system for determination of contaminants and impurities of heparin samples

Recent progress in the use of ionic polymers as pseudostationary phases for electrokinetic chromatography

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