Electromembrane extraction of amino acids from body fluids followed by capillary electrophoresis with capacitively coupled contactless conductivity detection

Strieglerová, Lenka; Kubáň, Pavel; Boček, Petr

doi:10.1016/j.chroma.2011.07.011

Cited by 122 publications

(51 citation statements)

References 43 publications

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“…Various enantioselective agents and their combinations were examined for optimization of baseline separations of the chiral isomers and the analysis of extracts of urine from athletes was demonstrated . Amino acids, which are usually derivatized and subsequently visualized using LIF detection, were detected in their bare cationic form in highly acidic buffer solutions in various body fluid samples . Large biomolecules, such as proteins, can also be sensitively determined in their underivatized form in CE‐C 4 D. Protonation of proteins and their determination as cations was achieved in acetic acid and seven proteins were determined after addition of micromolar concentrations of an ionic liquid to the buffer solution for their better resolution, recovery, and detection sensitivity .…”

Section: Applications Of Electrophoresis Methods With Conventional Camentioning

confidence: 99%

Contactless conductivity detection for analytical techniques: Developments from 2010 to 2012

Kubáň

Hauser

2012

Electrophoresis

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The developments in the field of capacitively coupled contactless conductivity detection in the approximate period from July 2010 to June 2012 are traced. Few reports concerning fundamental studies or new detector designs have appeared. On the other hand, applications in standard CZE are flourishing and contactless conductivity measurements are increasingly being employed as part of novel or more sophisticated experimental systems. Work on the lab-on-chip devices integrating contactless conductivity detection is continuing. A range of reports on the use of the simple yet powerful detection technique of contactless conductivity measurements in chromatographic separation as well as for analytical methods not including a separation step have also appeared.

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Section: Applications Of Electrophoresis Methods With Conventional Camentioning

confidence: 99%

Contactless conductivity detection for analytical techniques: Developments from 2010 to 2012

Kubáň

Hauser

2012

Electrophoresis

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“…In most cases, di(2‐ethylhexyl) phosphate (DEHP) has been used, and ionic interactions between the analyte and DEHP at the SLM interface has been found to facilitate the mass transfer . Thus, with DEHP mixed with NPOE or 1‐octanol, small cations , amino acids , peptides , and basic drugs in the log P range of –0.4 to 2.0 have been extracted successfully by EME. Clearly, with DEHP in the SLM, ionic interactions are involved, and this opens up the permeability for more polar species.…”

Section: Mass Transfer In Eme With Different Liquid Membranesmentioning

confidence: 99%

Mass transfer in electromembrane extraction—The link between theory and experiments

Huang

Jensen

Seip

et al. 2015

J of Separation Science

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Electromembrane extraction was introduced in 2006 as a totally new sample preparation concept for the extraction of charged analytes present in aqueous samples. Electromembrane extraction is based on electrokinetic migration of the analytes through a supported liquid membrane and into a μL-volume of acceptor solution under the influence of an external electrical field. To date, electromembrane extraction has mostly been used for the extraction of drug substances, amino acids, and peptides from biological fluids, and for organic micropollutants from environmental samples. Electromembrane extraction has typically been combined with chromatography, mass spectrometry, and electrophoresis for analyte separation and detection. At the moment, close to 125 research papers have been published with focus on electromembrane extraction. Electromembrane extraction is a hybrid technique between electrophoresis and liquid-liquid extraction, and the fundamental principles for mass transfer have only partly been investigated. Thus, although there is great interest in electromembrane extraction, the fundamental principle for mass transfer has to be described in more detail for the scientific acceptance of the concept. This review summarizes recent efforts to describe the fundamentals of mass transfer in electromembrane extraction, and aim to give an up-to-date understanding of the processes involved.

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“…Therefore, it is needed to extract and enrich the amino acids in the tobacco and tobacco smoke before derivatization and HPLC. There are many methods are used to separate amino acids including precipitation [11], ion exchange [3], electromembrane extraction [12], liquidliquid extraction [13,14], solid phase extraction [15] and electrodialysis [16], etc. In view of solid-phase extraction (SPE) has the characteristics of time-saving, separation and enrichment of analytes so that it becomes the key step in the analysis of trace compounds in samples with complex matrices.…”

Section: Introductionmentioning

confidence: 99%

Preparation of molecularly imprinted polymers using theanine as dummy template and its application as SPE sorbent for the determination of eighteen amino acids in tobacco

Zhu

Wang

Zhu

et al. 2016

Talanta

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Electromembrane extraction of amino acids from body fluids followed by capillary electrophoresis with capacitively coupled contactless conductivity detection

Cited by 122 publications

References 43 publications

Contactless conductivity detection for analytical techniques: Developments from 2010 to 2012

Contactless conductivity detection for analytical techniques: Developments from 2010 to 2012

Mass transfer in electromembrane extraction—The link between theory and experiments

Preparation of molecularly imprinted polymers using theanine as dummy template and its application as SPE sorbent for the determination of eighteen amino acids in tobacco

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