Electron Microscope Observations on Tingible Body Macrophages in Mouse Spleen

Swartzendruber, D. C.; Congdon, C. C.

doi:10.1083/jcb.19.3.641

Cited by 80 publications

(40 citation statements)

References 8 publications

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“…Early light and electron microscopic studies revealed that these characteristic phagocytes contain many tingible bodies of lymphocyte origin in their cytoplasm, which led to their naming as TBM (53,54). The use of CD68 Abs to investigate GC macrophages in this study brought out results that conflict with previous research using Abs specific to the Mac-2 Ag (21).…”

Section: Gc Macrophagescontrasting

confidence: 55%

Is There a Typical Germinal Center? A Large-Scale Immunohistological Study on the Cellular Composition of Germinal Centers during the Hapten-Carrier–Driven Primary Immune Response in Mice

Wittenbrink

Klein

Weiser

et al. 2011

The Journal of Immunology

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Germinal centers (GCs) are complex, multicell-type, transient structures that form in secondary lymphatic tissues in response to T cell-dependent stimulation. This process is crucial to the adaptive immune response because it is the source of affinity maturation and long-lived B cell memory. Our previous studies showed that the growth of murine splenic GCs is nonsynchronized, involving broad-volume distributions of individual GCs at any time. This raises the question whether such a thing as a typical GC exists. To address this matter, we acquired large-scale confocal data on GCs throughout the course of the 2-phenyl-5-oxazolone chicken serum albumin-driven primary immune response in BALB/c mice. Semiautomated image analysis of 3457 GC sections revealed that, although there is no typical GC in terms of size, GCs have a typical cellular composition in that the cell ratios of resident T cells, macrophages, proliferating cells, and apoptotic nuclei are maintained during the established phase of the response. Moreover, our data provide evidence that the dark zone (DZ) and light zone (LZ) compartments of GCs are about the same size and led us to estimate that the minimal cell loss rate in GCs is 3% per hour. Furthermore, we found that the population of GC macrophages is larger and more heterogeneous than previously thought, and that despite enrichment of T cells in the LZ, the DZ of murine splenic GCs is not poor in T cells. DZ and LZ differ in the T cell-to-macrophage ratio rather than in the density of T cells.

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Section: Gc Macrophagescontrasting

confidence: 55%

Is There a Typical Germinal Center? A Large-Scale Immunohistological Study on the Cellular Composition of Germinal Centers during the Hapten-Carrier–Driven Primary Immune Response in Mice

Wittenbrink

Klein

Weiser

et al. 2011

The Journal of Immunology

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“…Staining of fragmented nuclei by MAb 12D11 was noted in some germinal centers Fig. 2G), possibly represents with cells undergoing apoptosis, a common event in germinal centers (24). In contrast, nuclear fragments in necrotic regions of tumors did not stain with MAb 12D11 (data not shown).…”

Section: Immunostaining Of Benign Tissuesmentioning

confidence: 93%

Monoclonal Antibody Specific for Histone H1 Phosphorylated by Cyclin-Dependent Kinases: A Novel Immunohistochemical Probe of Proliferation and Neoplasia

et al. 2002

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Monoclonal antibody 12D11 (MAb 12D11) has been shown to bind histone H1 isolated from human placenta and other tissues but not histone H1 that has been digested with bacterial alkaline phosphatase. We show here that phosphorylation of phosphatase-treated histone H1 with cyclin dependent-kinase (CDK) restores binding by MAb 12D11. We conclude that MAb 12D11 selectively binds histone H1 that has been phosphorylated by CDKs, and we have investigated the use of MAb 12D11 as an immunohistochemical probe of CDK activity in situ. Previous immunofluorescence studies have revealed strong nuclear staining by MAb 12D11 in proliferating cultured cells and the absence of staining in terminally differentiated cells. Immunohistochemical staining of frozen and formalin-fixed, paraffin-embedded sections of benign tissues with MAb 12D11 was nuclear and confined to recognized foci of cell proliferation. In lymphoid germinal centers, MAb 12D11 preferentially stained large lymphoid cells with a relative lack of staining in small cleaved cells, contrasting with a lack of cell size discrimination observed with the monoclonal antibody proliferation probe, MIB-1. Tumor tissues displayed strong albeit heterogeneous staining of malignant cells by MAb 12D11, with little or no staining observed in surrounding nonneoplastic stromal cells. Differential staining by MAb 12D11 of invasive and in situ carcinoma suggest applications in prognostication. MAb 12D11 may also be useful in identification of tumors more likely to respond to therapeutic CDK inhibitors.

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“…They show the typical histological features of apoptotic bodies, and examination of electron micrographs published by Swartzendruber and Congdon (1963) indicates to us that they undergo the classic sequence of changes following phagocytosis. Many of them have been shown to be derived from cells that have recently synthesized DNA (Fliedner, 1967;Odartchenko et al, 1967) and it has been suggested that cell death in lymphoid follicles might be an inevitable consequence of rapid cell proliferation (Yoffey and Courtice, 1970).…”

Section: The Morphology Of Apoptosismentioning

confidence: 99%

Apoptosis: A Basic Biological Phenomenon with Wideranging Implications in Tissue Kinetics

Kerr¹,

Wyllie²,

Currie³

1972

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Summary.-The term apoptosis is proposed for a hitherto little recognized mechanism of controlled cell deletion, which appears to play a complementary but opposite role to mitosis in the regulation of animal cell populations. Its morphological features suggest that it is an active, inherently programmed phenomenon, and it has been shown that it can be initiated or inhibited by a variety of environmental stimuli, both physiological and pathological.The structural changes take place in two discrete stages. The first comprises nuclear and cytoplasmic condensation and breaking up of the cell into a number of membrane-bound, ultrastructurally well-preserved fragments. In the second stage these apoptotic bodies are shed from epithelial-lined surfaces or are taken up by other cells, where they undergo a series of changes resembling in vitro autolysis within phagosomes, and are rapidly degraded by lysosomal enzymes derived from the ingesting cells.Apoptosis seems to be involved in cell turnover in many healthy adult tissues and is responsible for focal elimination of cells during normal embryonic development. It occurs spontaneously in untreated malignant neoplasms, and participates in at least some types of therapeutically induced tumour regression. It is implicated in both physiological involution and atrophy of various tissues and organs. It can also be triggered by noxious agents, both in the embryo and adult animal.

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Electron Microscope Observations on Tingible Body Macrophages in Mouse Spleen

Cited by 80 publications

References 8 publications

Is There a Typical Germinal Center? A Large-Scale Immunohistological Study on the Cellular Composition of Germinal Centers during the Hapten-Carrier–Driven Primary Immune Response in Mice

Is There a Typical Germinal Center? A Large-Scale Immunohistological Study on the Cellular Composition of Germinal Centers during the Hapten-Carrier–Driven Primary Immune Response in Mice

Monoclonal Antibody Specific for Histone H1 Phosphorylated by Cyclin-Dependent Kinases: A Novel Immunohistochemical Probe of Proliferation and Neoplasia

Apoptosis: A Basic Biological Phenomenon with Wideranging Implications in Tissue Kinetics

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