Electron microscopic and immunohistochemical examination of scarred human cornea re-treated by excimer laser

Abstract

Effects of argon laser treatment of the cornea must be regarded as a process acting over many months. Intra- and extracellular structures and components are involved and influence the unpredictable shape of the corneal architecture.

Quantitative Assessment of Ultrastructure and Light Scatter in Mouse Corneal Debridement Wounds

“…Figure 10 shows stromal gene expression as determined by Q-PCR. The results indicate significant upregulation of mRNA of the quiescent ECM proteins, decorin, 32 keratocan, 33 and lumican, 34 as well as proteins previously associated with wound healing and fibrosis: collagen III 35 and SPARC. 36 Expression was greater at 2 weeks than at 4 weeks after wounding for all these genes.…”

“…The upregulation of mRNA for collagen III 35 and biglycan, 43 as well as for the matricellular protein SPARC, 36 followed a pattern similar to that of the proteoglycans-increased at 2 weeks and less so at 4 weeks (Fig. 10).…”

Morphologic and Confocal Investigation on Salzmann Nodular Degeneration of the Cornea

“…In addition, stromal cells had no degenerative changes. 11 On the contrary, their ultrastructural features (large number of RER cisternae, evident nucleoli, and many deep invaginations of the nuclear membrane) resembled those of the activated fibroblasts 25 or of the myofibroblasts 19 of the anterior stroma during corneal fibrotic repair. 26 These morphologic data were strongly supported by the confocal images.…”

Delayed corneal epithelial healing after hyperopic laser-assisted subepithelial keratectomy in patients with accommodative esotropia

“…5,6 The full return of normal structure and function can take months, even years, according to the type of surgery and individual variations in the wound-healing response. The other factors that might influence wound healing and corneal surface remodeling include the surgical technique used, 6,7 excimer laser systems, [8][9][10][11][12] and ocular movements, resulting in irregular ablated surfaces, delayed epithelial healing, and altered stromal remodeling. 13 Delayed corneal wound healing after LASEK for high hyperopia is presumably the result of variable causes:…”