Electron Microscopic Study of Intercalated Duct Cells in the Chicken Pancreatic Islet and Effects of Tolbutamide Administration

Mutoh, Ken-ichiro; Wakuri, Hidekazu; Liu, Bo; Seno, Makoto; Taniguchi, Kazumi

doi:10.2535/ofaj1936.75.5_231

Cited by 8 publications

(7 citation statements)

References 22 publications

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“…It can be assumed that during morphogenesis of the squamate pancreas, they become dynamic (Baum & Georgiou, ; Takeichi, ) and allow the cells' shape and even their location to change (Lecuit, ). The structure of junctional complexes during pancreas differentiation in grass snake is similar those of all other vertebrate species, both in embryos (Pan & Wright, ; Pictet & Rutter, ; Wessells & Evans, ) and adults (Farquhar & Palade, ; Friend & Gilula, ; Kojima et al, ; Mutoh, Wakuri, Liu, Seno, & Taniguchi, ; Vogelbein & Fournie, ). Therefore, it can be assumed that the function of cellular connections between cells forming the pancreatic ducts of the grass snake is the same as in other vertebrates.…”

Section: Discussionsupporting

confidence: 52%

Development of the duct system during exocrine pancreas differentiation in the grass snake Natrix natrix (Lepidosauria, Serpentes)

Kowalska

Rupik

2018

Journal of Morphology

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We analyzed the development of the pancreatic ducts in grass snake Natrix natrix L. embryos with special focus on the three-dimensional (3D)-structure of the duct network, ultrastructural differentiation of ducts with attention to cell types and lumen formation. Our results indicated that the system of ducts in the embryonic pancreas of the grass snake can be divided into extralobular, intralobular, and intercalated ducts, similarly as in other vertebrate species. However, the pattern of branching was different from that in other vertebrates, which was related to the specific topography of the snake's internal organs. The process of duct remodeling in Natrix embryos began when the duct walls started to change from multilayered to single-layered and ended together with tube formation. It began in the dorsal pancreatic bud and proceeded toward the caudal direction. The lumen of pancreatic ducts differentiated by cavitation because a population of centrally located cells was cleared through cell death resembling anoikis. During embryonic development in the pancreatic duct walls of the grass snake four types of cells were present, that is, principal, endocrine, goblet, and basal cells, which is different from other vertebrate species. The principal cells were electron-dense, contained indented nuclei with abundant heterochromatin, microvilli and cilia, and were connected by interdigitations of lateral membranes and junctional complexes. The endocrine cells were electron-translucent and some of them included endocrine granules. The goblet cells were filled with large granules with nonhomogeneous, moderately electron-dense material. The basal cells were small, electron-dense, and did not reach the duct lumen.

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Section: Discussionsupporting

confidence: 52%

Development of the duct system during exocrine pancreas differentiation in the grass snake Natrix natrix (Lepidosauria, Serpentes)

Kowalska

Rupik

2018

Journal of Morphology

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“…Several papers have suggested that folliculo-stellate cells may be involved in phagocytosis, support, contraction, stem cell function, and paracrine regulation of hormone secretion. Mutoh et aL (1998) found that intercalated duct cells darkened and increased lysosomes in number and size in the cytoplasm after tolbutamide treatment, as they support to endocrine islet cells. The intercalated duct cells of the pancreas which penetrate through the islets, may perform functions similar to those of folliculo-stellate cells in relation to islet endocrine cells.…”

Section: Resultsmentioning

confidence: 85%

Intercalated Duct Cells in the Moorhen (Gallinula chloropus) Pancreatic Islet

Mutoh

Wakuri

Taniguchi

1999

Okajimas Folia Anatomica Japonica

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Summary: Intercalated duct cells are present in the alpha and beta islets of the moorhen pancreas. The intercalated duct cells adhere to each other via intercellular junctional complexes on the apical side, projecting many microvilli into the lumen. They also extend slender cytoplasmic processes between islet endocrine cells. These intercalated duct cells appear to have a stellate shape, and to wrap their cytoplasm around the endocrine cells. These observations suggest that intercalated ducts not only pass through the islets, but also play a role in support to islet cells.

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“…Earlier work on chick pancreas (Mutoh et al, 1997(Mutoh et al, , 1998(Mutoh et al, , 1999 clearly indicated presence of the stellate cells in chick A and B islets as a part of intercalated duct cells, probably excluding the possibility of epithelial mesenchymal transition, which needs to be verified.…”

Section: Discussionmentioning

confidence: 97%

“…Electron microscopic studies of these cells have suggested their role in protecting islets against alloxan insults (Mutoh et al, 1997(Mutoh et al, , 1998 and their importance in protecting the islet endocrine cells against the diabetogenic insults in chicken. A possible role in phagocytosis, support, contraction, stem cell function, and paracrine regulation of hormone secretion has also been suggested (Mutoh et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Islet-derived stellate-like cells as a novel source for islet neogenesis in chicks

Datar

Bhonde

2009

Poultry Science

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The therapeutic potential of stem cells has led to renewed interest in regenerative biology. Pancreatic stellate cells have been reported in the mammalian pancreas; however, there are very few reports on stellate cells in the chicken pancreas. The intercalated duct epithelial cells observed in the A and B islets of the chicken pancreas have been claimed to be stellate cells from their morphological appearance. While isolating islets and acinar cells from the chick pancreas, we have found a population of stellate-like cells, which has been successfully propagated in a defined nutrient medium. These cells were immunopositive for vimentin, desmin, and fibronectin and also expressed alkaline phosphatase, indicating their undifferentiated state. On exposure to serum-free medium containing specific nutrients and differentiating agents, these stellate-like-cells gave rise to islet-like cell clusters. Islet-like clusters stained positive for the islet specific stain diphenyl thiocarbazone and were immunopositive for C-peptide indicating de novo insulin synthesis. These clusters secreted insulin in response to glucose challenge, thus suggesting their similarity to islets. Thus stellate cells found in chick pancreatic islets exhibit potential to differentiate into islet-like clusters. Taken together, our study documents for the first time the presence of a stellate-like cell population in chick pancreatic islets providing a source for islet neogenesis.

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Electron Microscopic Study of Intercalated Duct Cells in the Chicken Pancreatic Islet and Effects of Tolbutamide Administration

Cited by 8 publications

References 22 publications

Development of the duct system during exocrine pancreas differentiation in the grass snake Natrix natrix (Lepidosauria, Serpentes)

Development of the duct system during exocrine pancreas differentiation in the grass snake Natrix natrix (Lepidosauria, Serpentes)

Intercalated Duct Cells in the Moorhen (Gallinula chloropus) Pancreatic Islet

Islet-derived stellate-like cells as a novel source for islet neogenesis in chicks

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