Electron Microscopy and Restriction Endonuclease Analysis of RSF2124-pLS11 Composite Plasmid ConstructedIn Vitro

A Spontaneous, Ampicillin Resistant and Tetracycline Sensitive Mutant-Plasmid pTA2070 Derived from pBR322

“…These molecules were mixed, denatured and renatured, spread for electron microscopy in 56 % formamide solution over 18 % formamide hypophase, and visualized at a magnification of 10,000 in a Hitachi HU-12 electron microscope, as detailed previously. 7) In the heteroduplex molecule between the two linear molecules, a single insertion loop was seen (indicated by the arrow). The site of insertion was located at 10.1 % length of pBR322 calculated from the Sail cleavage site.…”