Electron microscopy for imaging organelles in plants and algae

Weiner, Ethan R.; Pinskey, Justine M; Otegui, Marisa S.

doi:10.1093/plphys/kiab449

Cited by 29 publications

(19 citation statements)

References 145 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This key information will help us to better understand the capacities of plant metabolic networks as a whole in relation to plant development. It is especially important to consider the bigger picture given our current capacity to massively produce data using sophisticated modern tools such as plant phenotyping platforms, imaging techniques (Midorikawa et al, 2022 ; Oi et al, 2017 ; Pipitone et al, 2021 ; Weiner et al, 2022 ), segmentation tools (Harline & Roeder, 2023 ; Wolny et al, 2020 ), proteomics (Mergner et al, 2020 ), laser microdissection (Balasubramanian et al, 2021 ) and single-cell analyses (de Souza et al, 2020 ; Seyfferth et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

The Arabidopsis leaf quantitative atlas: a cellular and subcellular mapping through unified data integration

Tolleter,

Smith,

Dupont-Thibert

et al. 2024

Quant Plant Bio.

View full text Add to dashboard Cite

Quantitative analyses and models are required to connect a plant’s cellular organisation with its metabolism. However, quantitative data are often scattered over multiple studies, and finding such data and converting them into useful information is time-consuming. Consequently, there is a need to centralise the available data and to highlight the remaining knowledge gaps. Here, we present a step-by-step approach to manually extract quantitative data from various information sources, and to unify the data format. First, data from Arabidopsis leaf were collated, checked for consistency and correctness and curated by cross-checking sources. Second, quantitative data were combined by applying calculation rules. They were then integrated into a unique comprehensive, referenced, modifiable and reusable data compendium representing an Arabidopsis reference leaf. This atlas contains the metrics of the 15 cell types found in leaves at the cellular and subcellular levels.

show abstract

Section: Discussionmentioning

confidence: 99%

The Arabidopsis leaf quantitative atlas: a cellular and subcellular mapping through unified data integration

Tolleter,

Smith,

Dupont-Thibert

et al. 2024

Quant Plant Bio.

View full text Add to dashboard Cite

show abstract

“…For example, the use of different fluorescent dyes in live-cell imaging and the ability to work with large fields of view allowed us to rapidly identify vacuolar compartments in the bulk of the endosperm. However, conventional live-cell imaging is limited by its resolution (200 nm in the x-y plane) and also by the fact that only fluorescent signals can be detected while unlabeled molecules or organelles cannot be visualized with sufficient quality (Weiner et al, 2022). The superior resolution of electron microscopy plus the use of heavy-metals in sample preparation that contrast the entire cell, allowing the ultrastructure of the whole cell to be examined at once (Martell et al, 2017), makes electron microscopy the perfect complement to live-cell imaging and enables structural research across the scales.…”

Section: Discussionmentioning

confidence: 99%

Multiscale imaging reveals the presence of autophagic vacuoles in developing maize endosperm

2023

View full text Add to dashboard Cite

Cereal endosperm is solely devoted to the storage of proteins and starch that will be used by the embryo upon germination. The high degree of specialization of this tissue is reflected in its endomembrane system, in which ER derived protein bodies and protein storage vacuoles (PSVs) are of particular interest. In maize seeds, the main storage proteins are zeins, that form transport incompetent aggregates within the ER lumen and finally build protein bodies that bud from the ER. In contrast to the zeins, the maize globulins are not very abundant and the vacuolar storage compartment of maize endosperm is not fully described. Whereas in other cereals, including wheat and barley, the PSV serves as the main protein storage compartment, only small, globulin-containing PSVs have been identified in maize so far. We present here a multi-scale set of data, ranging from live-cell imaging to more sophisticated 3D electron microscopy techniques (SBF-SEM), that has allowed us to investigate in detail the vacuoles in maize endosperm cells, including a novel, autophagic vacuole that is present in early developmental stages.

show abstract

“…CLEM is beginning to be applied to the endomembrane structures in plant cells as well, and is under improvement thanks to the development of related methods such as recovering of more fluorescent signal after fixation or automatic system to support correlation (Toyooka and Shinozaki-Narikawa, 2019;Wang et al, 2019). Although there are still some difficulties in sample preparation for applying the same 3D electron microscopy methods like FIB-SEM to vascular plants because of the plant-specific reasons such as the large cell size and the presence of huge vacuoles (Liu et al, 2020;Weiner et al, 2021), technical advancement would overcome them. Nevertheless, as the dynamics information is lost in CLEM, the improvement in light microscopy is yet required.…”

Section: Microscopic Approaches For the Remaining Problems In Plant E...mentioning

confidence: 99%

“…As we can see from the historic works from the group of George Palade, Albert Claude, and Keith Porter, the discoveries made by the electron microscopy literally formed the foundation of modern cell biology (Claude, 1975;Sabatini, 1999;Tartakoff, 2002;Satir, 2005). Since then, the electron microscopy has been contributing a lot for the investigation of membrane traffic within the cells including plant cells (Otegui and Pennington, 2019;Liu et al, 2020;Weiner et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Super resolution live imaging: The key for unveiling the true dynamics of membrane traffic around the Golgi apparatus in plant cells

Ito

Uemura

2022

Front. Plant Sci.

View full text Add to dashboard Cite

In contrast to the relatively static image of the plants, the world inside each cell is surprisingly dynamic. Membrane-bounded organelles move actively on the cytoskeletons and exchange materials by vesicles, tubules, or direct contact between each other. In order to understand what is happening during those events, it is essential to visualize the working components in vivo. After the breakthrough made by the application of fluorescent proteins, the development of light microscopy enabled many discoveries in cell biology, including those about the membrane traffic in plant cells. Especially, super-resolution microscopy, which is becoming more and more accessible, is now one of the most powerful techniques. However, although the spatial resolution has improved a lot, there are still some difficulties in terms of the temporal resolution, which is also a crucial parameter for the visualization of the living nature of the intracellular structures. In this review, we will introduce the super resolution microscopy developed especially for live-cell imaging with high temporal resolution, and show some examples that were made by this tool in plant membrane research.

show abstract

Electron microscopy for imaging organelles in plants and algae

Abstract: Sentence summary Recent advances in three-dimensional electron microscopy (3D-EM), including available instrumentation and best procedures for sample preparation, have drastically impacted our understanding of organelle function and plant cell biology.

Cited by 29 publications

References 145 publications

The Arabidopsis leaf quantitative atlas: a cellular and subcellular mapping through unified data integration

The Arabidopsis leaf quantitative atlas: a cellular and subcellular mapping through unified data integration

Multiscale imaging reveals the presence of autophagic vacuoles in developing maize endosperm

Super resolution live imaging: The key for unveiling the true dynamics of membrane traffic around the Golgi apparatus in plant cells

Contact Info

Product

Resources

About