Electron microscopy of the localization of formic dehydrogenase in regenerating forms ofProteus vulgaris

TETRANITRO-BLUE TETRAZOLIUM REDUCTION IN PROTEUS VULGARIS

“…VanderWinkel and Murray (6), treating Escherichia toll and Spirillum sopcns with 2,3,5-triphenyl tetrazolium, observed completely empty spaces which were explained by the dissolution of formazan deposits. Applying nitro-blue tetrazolium (NBT) to "regenerating forms" of Proteus vulgaris, Nermut (7) counted in the electron microscope an average of four empty areas per cell section, whereas in the sections of the control cells the average number was 0.05. Nermut, in his case, interpreted these empty holes as the sites where originally the NBT formazan had been, and which he believed to be sublimated subsequently in the electron microscope.…”