Electron microscopy ofin vitro cultured cells. A new method, based on the use of a Teflon film as cell support

Ewijk, Willem van; Hösli, P.

doi:10.1007/bf01003789

Histochem J

1975

DOI: 10.1007/bf01003789

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Electron microscopy ofin vitro cultured cells. A new method, based on the use of a Teflon film as cell support

Willem van Ewijk

P. Hösli

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1979

1992

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Cited by 3 publications

References 7 publications

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Identification and study of the same cell in monolayer culture by different methods of light microscopy, scanning, and transmission electron microscopy. Application for cryodamaged cells examination

Katsen¹,

Tregubova²,

Andrushkevich³

et al. 1992

Scanning

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Cells were cultivated on transparent conductive substrates. glass slides coated with indium oxide: individual cells were marked with a diamond indentor. Cell cultures were frozen ( -15"C), thawed, and then stained with fluorescent dyes to determine cell damage. The marked cells were examined by phase contrast. fluorescence, and Nomarski DIC microscopy. After aldehyde and osmium tetroxide fixation, the cell preparations were sequentially treated with tannic acid, uranyl acetate, and lead citrate. The same marked cell could be sequentially studied by light microscopy (LM; in water immersion conditions), scanning electron microscopy (SEM; after dehydration and critical point drying), and transmission electron microscopy (TEM: after embedding of cell samples in epoxy resin and laser marking of the cell previously marked with a diamond indentor). The method used ensures good preservation of cell morphology, cell surface relief, and intracellular structures. The treatment used renders the cells conductive and permitted SEM of uncoated culture cells on conductive substrates.

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Identification and study of the same cell in monolayer culture by different methods of light microscopy, scanning, and transmission electron microscopy. Application for cryodamaged cells examination

Katsen¹,

Tregubova²,

Andrushkevich³

et al. 1992

Scanning

View full text Add to dashboard Cite

show abstract

A new exposure model for in vitro testing of effects of gaseous pollutants on mammalian cells by means of gas diffusion through plastic films

Alink¹,

Hoeven²,

Debets³

et al. 1979

Chemosphere

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In Situ Embedding of Cell Monolayers Cultured on Plastic Surfaces for Electron Microscopy

Ab¹,

Schulze²,

Blauw³

1991

Biotechnic & Histochemistry

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Various procedures suitable for routine in situ embedding of cell monolayers were tested including: (1) the use of different Epon substitutes, (2) the use of different types of plasticware obtained from different sources, and (3) different methods of preparing capsules for sectioning. Different resins reacted differently with different plastics and type of preparation. Merck Epon substitute bound to most of the plastics tested. Ladd Epon substitute released cleanly from all plastics tested when a suitable method of preparation was used. The results show that for routine embedding of cell monolayers it is necessary to select an appropriate Epon substitute and method of preparation of capsules for the type of plasticware used. A routine method is described, with various alternative steps which can be applied when particular difficulties are encountered.

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Electron microscopy ofin vitro cultured cells. A new method, based on the use of a Teflon film as cell support

Cited by 3 publications

References 7 publications

Identification and study of the same cell in monolayer culture by different methods of light microscopy, scanning, and transmission electron microscopy. Application for cryodamaged cells examination

Identification and study of the same cell in monolayer culture by different methods of light microscopy, scanning, and transmission electron microscopy. Application for cryodamaged cells examination

A new exposure model for in vitro testing of effects of gaseous pollutants on mammalian cells by means of gas diffusion through plastic films

In Situ Embedding of Cell Monolayers Cultured on Plastic Surfaces for Electron Microscopy

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