Electron paramagnetic resonance spectroscopy in radiation research: Current status and perspectives

Rana, Shilpa; Chawla, Raman; Kumar, Raj; Singh, Sandeep; Zheleva, A.; Dimitrova, Yanka; Gadjeva, V.; Arora, Rajesh; Sultana, Sarwat; Sharma, Rakesh Kumar

doi:10.4103/0975-7406.67006

Cited by 19 publications

(2 citation statements)

References 62 publications

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“…Thus, free radicals such as ROS can be monitored directly if they occur in high enough concentrations and have sufficient stability. Due to the short half-life and high reactivity of the ROS themselves, spin traps are generally employed to transform them to stable or long-lived detectable spin-active species with a distinctive line splitting pattern [26-28]. Several substances have been used as spin-trapping agents, and can be classified in three groups: compounds with nitrone, nitroso and piperidine/pyrrolidine groups [29].…”

Section: Electron Spin Resonance (Esr) and Spin Trapsmentioning

confidence: 99%

Use of fluorescent probes for ROS to tease apart Type I and Type II photochemical pathways in photodynamic therapy

García-Díaz

Huang

Hamblin

2016

Methods

106

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Photodynamic therapy involves the excitation of a non-toxic dye by harmless visible light to produce a long-lived triplet state that can interact with molecular oxygen to produce reactive oxygen species (ROS), which can damage biomolecules and kill cells. ROS produced by electron transfer (Type 1) include superoxide, hydrogen peroxide and hydroxyl radical (HO•), while singlet oxygen (1O2) is produced by energy transfer. Diverse methods exist to distinguish between these two pathways, some of which are more specific or more sensitive than others. In this review we cover the use of two fluorescence probes: singlet oxygen sensor green (SOSG) detects 1O2; and 4-hydroxyphenyl-fluorescein (HPF) that detects HO•. Interesting data was collected concerning the photochemical pathways of functionalized fullerenes compoared to tetrapyrroles, stable synthetic bacteriochlorins with and without central metals, phenothiazinium dyes interacting with inorganic salts such as azide.

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Section: Electron Spin Resonance (Esr) and Spin Trapsmentioning

confidence: 99%

Use of fluorescent probes for ROS to tease apart Type I and Type II photochemical pathways in photodynamic therapy

García-Díaz

Huang

Hamblin

2016

Methods

106

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“…In order to help minimize these consequences, researchers have sought to develop biodosimeters that can estimate the biological effects of ionizing radiation. Several different technical approaches have been proposed including detection of electron paramagnetic resonance in tissues [2], induced chromosomal abnormalities [3], altered gene expression [4] and modified circulating proteins [5].…”

Section: Introductionmentioning

confidence: 99%

Breath biomarkers of total body irradiation in non-human primates

Phillips¹,

Grün²,

Schmitt³

2022

J. Breath Res.

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Background: Radiation exposure causes oxidative stress, eliciting production of metabolites that are exhaled in the breath as volatile organic compounds (VOCs). We evaluated breath VOCs as potential biomarkers for use in radiation biodosimetry. Methods: Five anesthetized non-human primates receive total body irradiation (TBI) of three daily fractions of 120 cGy per day for three days, resulting in a cumulative dose of 10.8 Gy. Breath samples were collected prior to irradiation and after each radiation fraction, and analyzed with gas chromatography mass spectrometry. Results: TBI elicited a prompt and statistically significant increase in the abundance of several hundred VOCs in the breath, including some that were increased more than five-fold, with100% sensitivity and 100% specificity for radiation exposure. The most significant breath VOC biomarkers of radiation mainly comprised straight-chain n-alkanes (e.g. hexane), as well as methylated alkanes (e.g. 3-methyl-pentane) and alkane derivatives (e.g. 2-butyl-1-octanol), consistent with metabolic products of oxidative stress. An unidentified breath VOC biomarker increased more than ten-fold following TBI, and rose linearly with the total cumulative dose of radiation (R2=0.92). Conclusions: TBI of non-human primates elicited increased production of breath VOCs consistent with increased oxidative stress. These findings provide a rational basis for further evaluation of breath VOC biomarkers in human radiation biodosimetry.

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Passive Dosimeters for Radiation Dosimetry: Materials, Mechanisms, and Applications

Yang,

Vrielinck,

Jacobsohn

et al. 2024

Adv Funct Materials

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Passive dosimeters enabling accurate measurement of doses from gamma rays to visible light are necessary to ensure efficient utilization of electromagnetic radiation in numerous fields like medical diagnostics and industrial manufacturing. The specific requirements for dosimeters in terms of dosimetry range, sensitivity, accuracy, and stability for each application have led to the development of various dosimeters based on new materials and new mechanisms. Here, a comprehensive review of different types of dosimeters classified according to the response signal, namely electron paramagnetic resonance, electrical, and optical, is provided. This review starts with a general introduction to dosimetry, a classification of dosimeters, and an elucidation of the necessity of dosimeters in various ranges of the electromagnetic spectrum. This is followed by an overview of the fundamental requirements of dosimeters, an explanation of the general dosimetry procedure, and the dosimetric quantities. Emphasis is given to the working mechanism, the design concept, and applications of each type of dosimeter as well as their respective strengths and drawbacks. Challenges and prospects are presented at the end of the review. This review provides an insightful overview of the material‐mechanism‐characteristics‐application relationship of different types of dosimeters that hopefully can serve as inspiration for the development of new devices.

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Electron paramagnetic resonance spectroscopy in radiation research: Current status and perspectives

Cited by 19 publications

References 62 publications

Use of fluorescent probes for ROS to tease apart Type I and Type II photochemical pathways in photodynamic therapy

Use of fluorescent probes for ROS to tease apart Type I and Type II photochemical pathways in photodynamic therapy

Breath biomarkers of total body irradiation in non-human primates

Passive Dosimeters for Radiation Dosimetry: Materials, Mechanisms, and Applications

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