Electron probe microanalysis of the chemical elemental content of human follicular fluid

Chong, Augusto P.; Taymor, Melvin L.; Lechène, C.

doi:10.1016/0002-9378(77)90690-1

Cited by 15 publications

(7 citation statements)

References 4 publications

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“…Ca and Mg levels in FF samples obtained by puncturing follicles of ovaries in situ from patients undergoing laparotomy were similar to those of blood, with minimal differences [7]. These observations were supported by Gosden et al [8] who found that FF concentrations were highly comparable with serum and plasma levels in human concentrations of Ca and Mg in preovulatory follicles.…”

Section: Introductionsupporting

confidence: 78%

“…The Ca 2+ FF levels were also higher, but the values were not statistically significant. These findings are incompatible with those of Chong et al [7] and Gosden et al [8] who found that concentrations of Ca and Mg in preovulatory FF were highly comparable with serum levels. These differences may be due to the fact that we examined the ionized fraction in addition to the total level of Mg and Ca.…”

Section: Discussioncontrasting

confidence: 57%

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Divalent Cation Levels in Serum and Preovulatory Follicular Fluid of Women Undergoing in vitro Fertilization Embryo Transfer

Azem¹,

Hanannel²,

Wolf³

et al. 2004

Gynecol Obstet Invest

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In this controlled clinical study, we determined the serum and follicular fluid concentrations of the biologically active fractions of magnesium (Mg) and calcium (Ca) in 39 women undergoing controlled ovarian stimulation (COH) for in vitro fertilization (IVF) embryo transfer (ET). Serum levels of ionized Ca (Ca²⁺) and ionized Mg (Mg²⁺) were measured during day 3 of the IVF cycle, on the day of ovum pickup and 12 days following ET. Follicular fluid levels were measured on the day of ovum pickup. Serum levels of Mg²⁺ decreased and Ca²⁺ levels increased during the IVF cycle. Mg levels were significantly higher in follicular fluid than in serum. Ca²⁺ and Mg²⁺ may play a role in the preovulatory follicle, a possibility that warrants further study.

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Section: Introductionsupporting

confidence: 78%

Section: Discussioncontrasting

confidence: 57%

Divalent Cation Levels in Serum and Preovulatory Follicular Fluid of Women Undergoing in vitro Fertilization Embryo Transfer

Azem¹,

Hanannel²,

Wolf³

et al. 2004

Gynecol Obstet Invest

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“…Earlier studies on follicular fluid concentrations of sodium, potassium and chloride suggest that transudation is important [1,2], How ever, the many studies on follicular fluid steroids confirm that the follicle is a reser voir for the active granulosa and theca lu tein cells [3][4][5],…”

Section: Introductionmentioning

confidence: 99%

Human Follicular Fluid Levels of Calcium, Copper and Zinc

Karunanithy

Edirisinghe

et al. 1987

Gynecol Obstet Invest

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Human follicular fluid was collected under laparoscopic vision from 33 follicles in patients stimulated with clomiphene-human menopausal gonadotrophin in an IVF programme. Thirty-one oocytes were obtained from 26 follicles from which clear follicular fluid was obtained, and 16 oocytes fertilized and cleaved. The follicular fluid was analysed for calcium, copper and zinc using atomic absorption spectroscopy. The mean levels of calcium, copper and zinc were 86.9 ± 11.6, 1.16 ± 0.29, and 0.72 ± 0.12 µg/ml, respectively. Their levels were not significantly different in the follicular fluid collected from follicles of different sizes, with or without oocyte, and follicles with oocytes that were fertilized and were unable to be fertilized. Therefore, the concentrations of these elements do not seem to reflect oocyte status or maturity.

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“…However, the scanning electron microscope is indispensable for analysing relatively large objects such as (1) foreign bodies removed from the eye and wounds, (2) plates, screws and Pathology (1979), 11, January various other objects implanted in tissues by surgeons, (3) bulk soft tissue specimens (ZS.-Nagy et a/., 1977) and pieces or thick sections of bone and calculi, (4) deposits from fluid samples evaporated onto filter paper, or freeze dried on crystal silicon or a beryllium support (Beeuwkes et al, 1977;Borland et al, 1976;Chong et al, 1977), and (5) ashed tissues and powder samples (Stump et a/., 1977).…”

Section: Equipmentmentioning

confidence: 99%

Invited Review. the technique and scope of electron-probe x-ray analysis in pathology

Ghadially

1979

Pathology

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Electron probe microanalysis of the chemical elemental content of human follicular fluid

Cited by 15 publications

References 4 publications

Divalent Cation Levels in Serum and Preovulatory Follicular Fluid of Women Undergoing in vitro Fertilization Embryo Transfer

Divalent Cation Levels in Serum and Preovulatory Follicular Fluid of Women Undergoing in vitro Fertilization Embryo Transfer

Human Follicular Fluid Levels of Calcium, Copper and Zinc

Invited Review. the technique and scope of electron-probe x-ray analysis in pathology

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