Electron transfer and fluorescence “turn-off” based CdTe quantum dots for vancomycin detection at nanogram level in aqueous serum media

Liang, Wanjun; Liu, Shaopu; Liu, Zhengqing; Li, Dan; Wang, Linlin; Hao, Chenxia; He, Yonghong

doi:10.1039/c4nj01764a

Cited by 22 publications

(8 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The slope of the curve increases with the increase in temperature, indicating that the quenching constant increases. All this evidence supports that the quenching mechanism for the interaction of BNCDs and curcumin is mainly initiated by a dynamic process and the IFE …”

Section: Resultssupporting

confidence: 77%

Boron and nitrogen co‐doped carbon dots as a sensitive fluorescent probe for the detection of curcumin

Bian

Wang

et al. 2017

Luminescence

View full text Add to dashboard Cite

In this present study, a fluorescent probe was developed to detect curcumin, which is derived from the rhizomes of the turmeric. We used a simple and economical way to synthesize boron and nitrogen co-doped carbon dots (BNCDs) by microwave heating. The maximum emission wavelength of the BNCDs was 450 nm at an excitation wavelength of 360 nm. The as-prepared BNCDs were characterized by multiple analytical techniques such as transmission electron microscopy, X-ray photoelectron spectroscopy, X-ray diffraction, and infrared spectroscopy. Curcumin is a biologically active constituent of turmeric [1] which exhibits some biological and pharmacological activities such as antiinflammatory, [2] anti-ischemic, [3] antibacterial, [4] hepatoprotective, [5] anticarcinogenic, [6] and anti-dyspeptic [7] properties. It has also been reported that curcumin resists the formation of free radicals effectively such as superoxide radicals and hydroxyl radicals in blood and body tissues. [8,9] Due to the widespread application of curcumin in pharmacology and clinical medicine, much attention has been paid by researchers. Therefore, many methods for the detection of curcumin have been developed, including capillary electrophoresis, [10] high-performance thin-layer chromatographic, [11] high-performance liquid chromatography, [12,13] voltammetry, [14] liquid-liquid microextraction, [15] liquid chromatography-mass spectrometry, [16,17] and electrochemical techniques. [18] However, the majority of these Abbreviations used: BNCD, boron and nitrogen co-doped carbon dot; BSA, bovine serum albumin; CD, carbon dot; IFE, inner filter effect; PBS, phosphatebuffered solution; IR, infra-red; QY, quantum yield; TEM, transmission electron microscopy; UV, ultraviolet; XPS, X-ray photoelectron spectroscopy; XRD, X-ray diffraction.

show abstract

Section: Resultssupporting

confidence: 77%

Boron and nitrogen co‐doped carbon dots as a sensitive fluorescent probe for the detection of curcumin

Bian

Wang

et al. 2017

Luminescence

View full text Add to dashboard Cite

show abstract

“…It was found that the IC 50 value was 13.74 ng/L, the IC 20 -IC 80 were 3.43-43.62 ng/L, and the IC 10 reached up to 1.07 ng/L according to the established IC-TRFIA for Van. The LOD value was 1.07 ng/L in the presented IC-TRFIA for Van, which is more sensitive (approximately 60-fold) compared with Kong et al (2017) established IC-ELISA for Van with LOD value of 0.06 ng/mL, and approximately 460-fold, 2.0 × 10 5 -fold and 2.5 × 10 5 -fold compare with Liang et al (2015), Sheng and Zhou (2017) and Usman and Hempel (2016) which were established fluorescence "turn-off" biosensor, two-dimensional liquid chromatography and HPLC for Van with LOD value of 0.46 ng/mL, 0.2 and 0.25 μg/mL respectively. In addition, it was slightly better than Liu, Yan, Hua, and Wang (2013), Hu et al (2018), Zhou et al (2015) and Sheng et al (2016) established IC-TRFIAs for thiacloprid, aflatoxin B 1 , chloramphenicol and diniconazole with LOD value of 1.9, 3.55, 8.0 and 21 ng/L, respectively.…”

Section: Ic-trfia Establishment Of Van and Its Analoguesmentioning

confidence: 80%

Establishment of an ultrasensitive indirect competitive time-resolved fluoroimmunoassay for vancomycin determination

Han

Dong

et al. 2019

Food and Agricultural Immunology

View full text Add to dashboard Cite

Vancomycin (Van) is a common antibiotic that pollutes food, feed and ecological environment. In the present study, an anti-Van polyclonal antibodies (pAbs) and Eu 3+-labeled goat anti-rabbit IgG monoclonal antibody based indirect competitive timeresolved fluoroimmunoassay (IC-TRFIA) was established for ultrasensitive determination of Van, and its half-maximum inhibition concentrations (IC 50) was 13.74 ng/L and the limit of detection (LOD) was 1.07 ng/L. The IC-TRFIA showed strong crossreactivity (CRs) of 36.2% for norvancomycin, and less than 0.1% for polymyxin B, kanamycin and ampicillin. It was found that the IC-TRFIA for Van spiked in different milk powder samples determination were with good accuracy, stability and repeatability, and its recoveries are 83.0-97.9%, also with its coefficient of variations (CVs) are 3.0-12.9% in intra-assay and inter-assay. The results showed that the established IC-TRFIA was promising for ultrasensitive determination of Van in food samples. Highlights (1) Prepared high-quality Van-KLH/BSA conjugates. (2) Obtained high activity and purify anti-Van polyclonal antibodies. (3) Prepared high-quality Eu 3+-labelled goat anti-rabbit IgG monoclonal antibody. (4) Firstly established an IC-TRFIA for Van based on the anti-Van polyclonal antibodies and Eu 3+-labelled goat anti-rabbit IgG monoclonal antibody. (5) The IC-TRFIA can be used for ultrasensitive determination of Van in milk powder samples.

show abstract

“…The absorption prole of the CdTe QDs also shows a broad range of UV-Vis light absorptivity . 67,68 The CdTe/NTU-9 nanocomposite offers synergistic improvements in overall light harvesting capability, as evidenced by its higher degree of energy absorption. The direct energy bandgap (E g ) values of the CdTe QDs, NTU-9, and CdTe/NTU-9 (calculated using Tauc's method) are estimated to be 2.40, 1.85, and 2.21 eV, respectively.…”

Section: Absorption Studiesmentioning

confidence: 99%

Efficient photocatalytic and photovoltaic applications with nanocomposites between CdTe QDs and an NTU-9 MOF

et al. 2017

View full text Add to dashboard Cite

show abstract

Electron transfer and fluorescence “turn-off” based CdTe quantum dots for vancomycin detection at nanogram level in aqueous serum media

Abstract: Mode of interaction of GSH-CdTe QDs with vancomycin and the mechanism of the fluorescence “turn-off” process.

Cited by 22 publications

References 45 publications

Boron and nitrogen co‐doped carbon dots as a sensitive fluorescent probe for the detection of curcumin

Boron and nitrogen co‐doped carbon dots as a sensitive fluorescent probe for the detection of curcumin

Establishment of an ultrasensitive indirect competitive time-resolved fluoroimmunoassay for vancomycin determination

Efficient photocatalytic and photovoltaic applications with nanocomposites between CdTe QDs and an NTU-9 MOF

Contact Info

Product

Resources

About