Electron transfer between cellobiose dehydrogenase and graphite electrodes

Larsson, T.; Elmgren, Maja; Lindquist, Sten‐Eric; Tessema, Merid; Gorton, Lo; Henriksson, Gunnar

doi:10.1016/0003-2670(96)00136-5

Cited by 54 publications

(34 citation statements)

References 27 publications

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“…One of the most attractive electrode elements for a potentially implantable BFC is a carbon material, which is cheap, abundant and biocompatible. To design this BFC, SPGE were chosen, as such electrodes are well-characterised [30] and widely used for bioelectrochemical studies of variety of enzymes including different CDH [17,28,31,32] and BOx [20,29], on which both bioelements showed excellent DET-based bioelectrocatalysis (Figures 2 and 3).…”

Section: Resultsmentioning

confidence: 99%

A Direct Electron Transfer‐Based Glucose/Oxygen Biofuel Cell Operating in Human Serum

et al. 2010

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We report on the fabrication and characterisation of the very first direct electron transfer‐based glucose/oxygen biofuel cell (BFC) operating in neutral glucose‐containing buffer and human serum. Corynascus thermophilus cellobiose dehydrogenase and Myrothecium verrucaria bilirubin oxidase were used as anodic and cathodic bioelements, respectively. The following characteristics of the mediator‐, separator‐ and membrane‐less, a priori, non‐toxic and simple miniature BFC, was obtained: an open‐circuit voltage of 0.62 and 0.58 V, a maximum power density of ca. 3 and 4 μW cm–2 at 0.37 and 0.19 V of cell voltage, in phosphate buffer and human serum, respectively.

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Section: Resultsmentioning

confidence: 99%

A Direct Electron Transfer‐Based Glucose/Oxygen Biofuel Cell Operating in Human Serum

et al. 2010

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“…The model of sequential electron transfer from cellobiose to the FAD domain and to a 1 e À acceptor strictly via the heme domain is known as the "electron-chain model" [23]. On the other hand, it was seen that the isolated FAD domain can directly transfer one electron to a 1 e À acceptor, which suggests that the hemecofactor is not necessarily involved in the reduction of 1 e À acceptors, but might have the role of an electron sink and that 1 e À acceptors can interact with the FAD directly [2,7,20,24]. This model is named electron-sink model and up-hill transfer of electrons from H r to F o /F r .…”

Section: Introductionmentioning

confidence: 97%

“…But H r can also be oxidized at a polarized electrode that acts as an artificial 1 e À acceptor, via a direct electron transfer (DET) mechanism [7 -10]. It was shown that only the heme domain is responsible for the DET communication with the electrode [21] and it is believed that the heme domain participates in the ET communication with 1 e À acceptors [2,22], even though it was shown that an Os 2þ/3þ -redox polymer was as efficient a 1 e À acceptor/mediator for the intact enzyme as for the separate FAD domain [7].…”

Section: Introductionmentioning

confidence: 99%

“…In this work the electrochemical characterization of the CDH from M. thermophilum was performed. On graphite, direct electron transfer (DET) was observed by measuring the amperometric response, i.e., bioelectrocatalytic current, of the CDH modified electrode in the presence of cellobiose or lactose [7,8]. The FAD domain (F O ) catalyzes the oxidation of cellobiose, which donates 2 electrons to F O resulting in the fully reduced form (F r ).…”

Section: Introductionmentioning

confidence: 99%

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Investigation of Graphite Electrodes Modified with Cellobiose Dehydrogenase from the Ascomycete Myriococcum thermophilum

et al. 2007

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The catalytic properties of cellobiose dehydrogenase (CDH) from the ascomycete fungus Myriococcum thermophilum adsorbed on a graphite electrode were investigated for a large variety of carbohydrate substrates. The effects of applied potential, pH and buffer composition were tested and optimized, and the most suitable conditions were used to evaluate the detection limit, linear range, and sensitivity of the sensor for different carbohydrates in the flow injection mode. Subsequently, the long term stability of the modified electrodes was determined. Additionally, the direct and mediated electron transfer between the active site of the enzyme and the electrode has been investigated by amperometric flow injection measurements in the absence and presence of the mediator 1,4-benzoquinone in the presence of cellobiose or lactose.

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“…Apart from the glucose, alcohols were also extensively studied as fuel at anode in e-BES catalyzed by non-specific or specific alcohol dehydrogenases [240,241]. Similarly, cellobiose [229][230][231][232][233], fructose [234,235], pyruvate [227], glycerol [227], hydrogen [238,239], etc., were also studied as fuels in e-BES. Various studies have been performed to enhance the current densities, to meet the complete oxidation of the fuel, to increase the stability and longetivity of the enzyme properties.…”

Section: Anodic Oxidizing Reactionsmentioning

confidence: 99%

Enzymatic Electrosynthesis: An Overview on the Progress in Enzyme- Electrodes for the Production of Electricity, Fuels and Chemicals

Satyawali¹

2013

J Microbial Biochem Technol

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Recent interest in the field of biocommodities production through bioelectrochemical systems has generated interest in the enzyme catalyzed redox reactions. Enzyme catalyzed electrodes are well established as sensors and power generators. However, a paradigm shift in recent science towards the production of useful chemicals has changed the face of biofuel cells, keeping the fuels or chemicals production in the upfront. This review article comprehensively presents the progress in the field of enzyme-electrodes for the production of electricity, fuels and chemicals with an aim to represent a practical outline for understanding the use of single or multiple redox enzymes as electrocatalysts for their electron transfer onto electrodes. It also provides the state-of-the-art information regarding the different existing processes to fabricate enzyme electrodes. Successfully-achieved electroenzymatic anodic and cathodic reactions are further discussed, together with their potential applications. Particular focus was made on the novel single/multiple enzyme systems towards product synthesis and other applications. Finally, techno-economic and environmental elements for industrial processing with enzyme catalyzed bioelectrochemical system (e-BES) are anticipated, in order to provide useful strategies for further development of this technology.

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Electron transfer between cellobiose dehydrogenase and graphite electrodes

Cited by 54 publications

References 27 publications

A Direct Electron Transfer‐Based Glucose/Oxygen Biofuel Cell Operating in Human Serum

A Direct Electron Transfer‐Based Glucose/Oxygen Biofuel Cell Operating in Human Serum

Investigation of Graphite Electrodes Modified with Cellobiose Dehydrogenase from the Ascomycete Myriococcum thermophilum

Enzymatic Electrosynthesis: An Overview on the Progress in Enzyme- Electrodes for the Production of Electricity, Fuels and Chemicals

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