This article presents the circularly polarized antenna operating over 28 GHz mm-wave applications. The suggested antenna has compact size, simple geometry, wideband, high gain, and offers circular polarization. Afterward, two-port MIMO antenna are designed to get Left Hand Circular Polarization (LHCP) and Right-Hand Circular Polarization (RHCP). Four different cases are adopted to construct two-port MIMO antenna of suggested antenna. In case 1, both of the elements are placed parallel to each other; in the second case, the element is parallel but the radiating patch of second antenna element are rotated by 180°. In the third case, the second antenna element is placed orthogonally to the first antenna element. In the final case, the antenna is parallel but placed in the opposite end of substrate material. The S-parameters, axial ratio bandwidth (ARBW) gain, and radiation efficiency are studied and compared in all these cases. The two MIMO systems of all cases are designed by using Roger RT/Duroid 6002 with thickness of 0.79 mm. The overall size of two-port MIMO antennas is 20.5 mm × 12 mm × 0.79 mm. The MIMO configuration of the suggested CP antenna offers wideband, low mutual coupling, wide ARBW, high gain, and high radiation efficiency. The hardware prototype of all cases is fabricated to verify the predicated results. Moreover, the comparison of suggested two-port MIMO antenna is also performed with already published work, which show the quality of suggested work in terms of various performance parameters over them.