Electronically Type-Sorted Carbon Nanotube-Based Electrochemical Biosensors with Glucose Oxidase and Dehydrogenase

Muguruma, Hitoshi; Hoshino, Tatsuya; Nowaki, Kohei

doi:10.1021/am506758u

Cited by 41 publications

(52 citation statements)

References 52 publications

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“…Muguruma et al designed a LBL assembled glucose sensor using either metallic (mSWCNT) or semi-conducting (sSWCNT) CNTs that were drop cast onto a plasma polymerized acetonitrile film (PPF) with enzyme. [104] Using sorted SWCNTs, they determined the mechanism of the reaction was that CNTs facilitated direct electron transfer at GOx/sSWCNT sensors. Furthermore, they also developed a glucose dehydrogenase (GDH) based sensor, where sSWCNTs were more sensitive and less resistive.…”

Section: 0 Enzyme Substratesmentioning

confidence: 99%

Recent trends in carbon nanomaterial-based electrochemical sensors for biomolecules: A review

Yang

Denno

Pyakurel

et al. 2015

Analytica Chimica Acta

494

263

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Carbon nanomaterials are advantageous for electrochemical sensors because they increase the electroactive surface area, enhance electron transfer, and promote adsorption of molecules. Carbon nanotubes (CNTs) have been incorporated into electrochemical sensors for biomolecules and strategies have included the traditional dip coating and drop casting methods, direct growth of CNTs on electrodes and the use of CNT fibers and yarns made exclusively of CNTs. Recent research has also focused on utilizing many new types of carbon nanomaterials beyond CNTs. Forms of graphene are now increasingly popular for sensors including reduced graphene oxide, carbon nanohorns, graphene nanofoams, graphene nanorods, and graphene nanoflowers. In this review, we compare different carbon nanomaterial strategies for creating electrochemical sensors for biomolecules. Analytes covered include neurotransmitters and neurochemicals, such as dopamine, ascorbic acid, and serotonin; hydrogen peroxide; proteins, such as biomarkers; and DNA. The review also addresses enzyme-based electrodes that are used to detect non-electroactive species such as glucose, alcohols, and proteins. Finally, we analyze some of the future directions for the field, pointing out gaps in fundamental understanding of electron transfer to carbon nanomaterials and the need for more practical implementation of sensors.

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Section: 0 Enzyme Substratesmentioning

confidence: 99%

Recent trends in carbon nanomaterial-based electrochemical sensors for biomolecules: A review

Yang

Denno

Pyakurel

et al. 2015

Analytica Chimica Acta

494

263

View full text Add to dashboard Cite

show abstract

“…The deposition parameters were as follows: power 150 W; pressure 0.6 Pa; exposure time 150 s. The immobilization procedure for enzyme GDH is described in the literature. (9) …”

Section: Fabrication Proceduresmentioning

confidence: 99%

“…A preliminary investigation was performed to determine the effect of the SWCNT electronic type on the amperometric characteristics of a biosensor with NAD-dependent GDH. (9) The sensing mechanism involves a signal peak produced by NADH oxidation caused by the enzymatic reaction:…”

Section: Glucose Sensingmentioning

confidence: 99%

“…(8) However, there have been only a few studies on amperometric sensors (catalytic oxidation for target molecules) with electronically typesorted SWCNTs. We previously demonstrated an amperometric enzyme biosensor; (9) however, the main focus was the combination of two catalysts of SWCNTs and an enzyme. We envision that a fundamental understanding of the electrochemical behavior and analytical applications of electronically type-sorted SWCNT will be achieved through further investigations.…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical Behavior and Analytical Applications of Electronically Type-Sorted Carbon Nanotube Electrode

2016

Sensors and Materials

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Keywords: electronically type-sorted single-walled carbon nanotube, plasma-polymerized film, electrochemical sensor, nicotinamide adenine dinucleotideThe electrochemical behavior and analytical applications of an electrode with electronically typesorted (metallic and semiconducting) single-walled carbon nanotubes (SWCNTs) were examined. The effect of the electronic type of SWCNTs on the electrochemical behavior of nicotinamide adenine dinucleotide (NADH) as a model agent was investigated. The electrode configuration contained SWCNTs sandwiched between 3-5-nm-thick acetonitrile plasma-polymerized thin films. Cyclic voltammetry measurements demonstrated that the current due to the NADH oxidation of the electrode with semiconducting SWCNTs was much larger than that for electrodes with only metallic or unsorted SWCNTs. Electrochemical impedance spectroscopy measurements and atomic force microscopy observations suggested that the electrode with semiconducting SWCNTs formed a better electron transfer network than the electrodes with metallic or unsorted SWCNTs. Chronocoulometry measurements indicated that there was no distinct difference between the diffusion coefficients of NADH with the metallic and semiconducting SWCNT electrodes. Therefore, it was concluded that the semiconducting SWCNT electrode is more suitable than the metallic and unsorted SWCNT electrodes for electrochemical oxidation in terms of catalysis and electron transport. The semiconducting SWCNT electrode exhibited excellent performance for the sensing application of NADH detection with a significantly wide linear range of 59-5800 μM compared with that for conventional NADH sensors. Other significant performance characteristics are a sensitivity of 176 μA mM −1 cm −2 (defined as the slope of the current vs concentration plot at +0.4 V), a detection limit of 1.1 μM [signal-to-noise ratio (S/N) = 3], and a response time of 6 s.

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“…Recently, enzyme labels, such as alkaline phosphatase (AP), horseradish peroxidase (HRP), glucose oxidase and β-galactosidase, have been widely used in many biochemical immunoassay [13][14][15][16]. Particularly, the cost of HRP is much lower than other enzyme labels, thus, HRP-labeled antibody has been commercially accepted to be a detector for bioassay.…”

Section: Introductionmentioning

confidence: 99%

Nanobody-based electrochemical immunoassay for Bacillus thuringiensis Cry1Ab toxin by detecting the enzymatic formation of polyaniline

Zhu

et al. 2015

Microchim Acta

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We describe an electrochemical immunoassay for the Cry1Ab toxin that is produced by Bacillus thuringiensis. It is making use of a nanobody (a heavy-chain only antibody) that was selected from an immune phage displayed library. A biotinylated primary nanobody and a HRP-conjugated secondary nanobody were applied in a sandwich immunoassay where horseradish peroxidase (HRP) is used to produce polyaniline (PANI) from aniline. PANI can be easily detected by differential pulse voltammetry at a working voltage as low as 40 mV (vs. Ag/AgCl) which makes the assay fairly selective. This immunoassay for Cry1Ab has an analytical range from 0.1 to 1000 ng mL -1 and a 0.07 ng mL -1 lower limit of detection. The average recoveries of the toxin from spiked samples are in the range from 102 to 114 %, with a relative standard deviation of <7.5 %. The results demonstrated that the assay represented an attractive alternative to existing immunoassays in enabling affordable, sensitive, robust and specific determination of this toxin.

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Electronically Type-Sorted Carbon Nanotube-Based Electrochemical Biosensors with Glucose Oxidase and Dehydrogenase

Cited by 41 publications

References 52 publications

Recent trends in carbon nanomaterial-based electrochemical sensors for biomolecules: A review

Recent trends in carbon nanomaterial-based electrochemical sensors for biomolecules: A review

Electrochemical Behavior and Analytical Applications of Electronically Type-Sorted Carbon Nanotube Electrode

Nanobody-based electrochemical immunoassay for Bacillus thuringiensis Cry1Ab toxin by detecting the enzymatic formation of polyaniline

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