Electropermeabilization in bladder cancer chemotherapy

Kubota, Yōko; Nakada, Teruhiro; Yanai, Hiroshi; Kakizaki, Hiroshi; Sasagawa, Isoji; Watanabe, Morihiro

doi:10.1007/s002800050539

Cited by 20 publications

(15 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4,24,25 Nevertheless, any increase in the CIS or MMC cytotoxicity is appreciated because bladder cancer is already sensitive to these drugs. Our study shows that electrochemotherapy was effective using intratumor MMC or CIS.…”

Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Experiments on Electrochemotherapy for Bladder Cancer

et al. 2015

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Experiments on Electrochemotherapy for Bladder Cancer

et al. 2015

View full text Add to dashboard Cite

show abstract

“…KK-47, a low-grade and non-metastatic bladder tumor cell line (11), was generously provided by Dr T. Masuko, Tohoku University (Sendai, Japan) and YTS-1, a high-grade and metastatic bladder tumor cell line (12) was a generous gift from Dr H. Kakizaki, Yamagata University (Yamagata, Japan). Cells were maintained in RPMI-1640 medium (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS) (PAA Laboratories, Morningside, Australia).…”

Section: Methodsmentioning

confidence: 99%

MUC1 carrying core 2 O-glycans functions as a molecular shield against NK cell attack, promoting bladder tumor metastasis

et al. 2012

View full text Add to dashboard Cite

Core 2 β-1,6-N-acetylglucosaminyltransferase (C2GnT) forms an N-acetylglucosamine branch in O-glycans (core 2 O-glycans) of cell surface glycoproteins. C2GnT-expressing bladder tumors acquire highly metastatic phenotypes by surviving longer in host blood circulation. However, the detailed mechanisms underlying this increased survival remain unclear. In this study, we report that the expression of C2GnT in bladder tumors positively correlates with tumor progression and that bladder tumor cell-surface mucin 1 (MUC1) carrying core 2 O-glycans plays an important role in the evasion from natural killer (NK) cell attack. In C2GnT-expressing bladder tumor cells, heavily core 2 O-glycosylated MUC1 carries poly-N-acetyllactosamine in its O-glycans and galectin-3 binds to MUC1 through this poly-N-acetyllactosamine. The binding of galectin-3 to poly-N-acetyllactosamine in MUC1 core 2 O-glycans attenuates the interaction of the tumor cells with NK cells and interferes with the access of tumor necrosis factor-related apoptosis-inducing ligand to the tumor cell surface. These effects of MUC1 carrying core 2 O-glycans on NK cell attack facilitate C2GnT-expressing tumor cells to evade NK cell immunity and survive longer in host blood circulation. We reveal that MUC1 carrying core 2 O-glycans thus functions as a molecular shield against NK cell attack, thereby promoting bladder tumor metastasis.

show abstract

“…In fact, this method enables the introduction of any non-permeant or weakly permeant molecules into cells [9]. Recently, this method has been used to enhance the cytotoxicity of antineoplastic drugs [10, 11, 12], and we have already shown that such enhancement is caused by the increased uptake of drugs by cells [7]. …”

Section: Discussionmentioning

confidence: 99%

“…Such electric fields have been used to facilitate the introduction of cytotoxic drugs or genes into cells [5, 6]. We have already shown that electropermeabilization induces a twofold increase in the concentration of bleomycin (BLM) in bladder cancer cells in vitro and in normal bladder tissues of rats in vivo [7]. The aim of the present investigation was to examine the possible enhancement of the antitumor effect of BLM by electropermeabilization in rats with bladder cancer.…”

Section: Introductionmentioning

confidence: 99%