Electrophysiological Effects ofBacillus sphaericusBinary Toxin on Cultured Mosquito Cells

Çökmüş, Cumhur; Davidson, Elizabeth W.; Cooper, Katelyn M.

doi:10.1006/jipa.1997.4660

Cited by 34 publications

(26 citation statements)

References 30 publications

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“…The electrophysiological effects of the native Bin toxin and its individual components, BinA and BinB, have been investigated in cultured C. pipiens cells using the patch clamp technique (Cokmus et al 1997). The authors reported a reduction in wholecell membrane resistance, suggesting that the toxin create pores or channels in the cell membrane.…”

Section: Mode Of Action Of Mosquitocidal Bac-terial Toxinsmentioning

confidence: 99%

Mosquitocidal bacterial toxins: diversity, mode of action and resistance phenomena

Charles

Nielsen-LeRoux

2000

Mem. Inst. Oswaldo Cruz

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Section: Mode Of Action Of Mosquitocidal Bac-terial Toxinsmentioning

confidence: 99%

Mosquitocidal bacterial toxins: diversity, mode of action and resistance phenomena

Charles

Nielsen-LeRoux

2000

Mem. Inst. Oswaldo Cruz

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“…However, amino acids essential for receptor binding have not been identified. Some evidences suggest that BinB is inserted into membrane and form pores (24)(25)(26), although the precise mechanism in which the toxin kills the target cells is unclear. Therefore, C67 and C161 could also play a part in conformational change, receptor binding, or membrane insertion.…”

Section: Effect Of Amino Acid Substitutions On Bina-binb Interactionmentioning

confidence: 99%

Role of cysteine at positions 67, 161 and 241 of a Bacillus sphaericus binary toxin BinB

Boonyos¹,

Soonsanga²,

Boonserm³

et al. 2010

BMB Reports

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Binary toxin consisting of BinA and BinB from Bacillus sphaericus is toxic to mosquito larvae. BinB is responsible for specific binding to the larval gut cell membrane while BinA is crucial for toxicity. To investigate functional role of cysteine in BinB, three cysteine residues at positions 67, 161, and 241 were replaced by alanine or serine. Mutations at these positions did not affect protein production and overall structure of BinB. These cysteine residues are not involved in disulfide bond formation between BinB molecules. Mosquito-larvicidal assays revealed that C67 and C161 are essential for toxicity, whereas C241 is not. Mutations at C67 and C161 resulted in weaker BinA-BinB interaction. The loss of toxicity may be due to the reduction of interactions between BinA and BinB or BinB and its receptor. C67 and C161 could also play a part during conformational changes or internalization of the binary toxin into the target cell. [BMB reports 2010; 43(1): 23-28]

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“…Estudios fisiológicos del Culex quinquefasciatus han evidenciado que la toxina binaria también actúa sobre la membrana celular en la cual puede causar poros como los producidos por el Bacillus thuringiensis que conllevan a la muerte celular (31)(32)(33).…”

Section: Inclusión Cristalinaunclassified

Bacillus sphaericus: biocontrolador de vectores que producen malaria, fiebre amarilla y dengue

Romero

Rodríguez

Ramírez

2009

nova

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ResumenEl aumento en la incidencia de enfermedades de interés en salud pública como malaria, dengue y fiebre amarilla asociado a los efectos secundarios del uso de plaguicidas químicos y la resistencia generada, ha impulsado a la búsqueda de nuevas alternativas para la erradicación de sus vectores. Una opción viable como bacteria entomopatógena es el Bacillus sphaericus, descrita por primera vez en 1904 por Neide y Meyer, quienes descubrieron una bacteria formadora de esporas, que existe en la naturaleza y que crece fácilmente tanto in vitro como en el cadáver de las larvas.Su actividad larvicida se debe a la producción de una inclusión cristalina denominada toxina binaria A y B de 41,9 y 51,4 kDa, respectivamente, las cuales, al ser liberadas en el intestino de los insectos susceptibles en su fase larvaria y tras ser solubilizada por el pH alcalino en la porción media del intestino, activan las proteasas causando deshidratación y muerte a los vectores. Es considerada como un biolarvicida altamente potencial y eficaz para el control biológico de Anopheles spp., Aedes spp. y Culex spp. Comercialmente puede producirse por fermentación sumergida a pequeña escala con materiales proteínicos de desecho. Este producto ha sido utilizado y registrado en numerosos países de América Latina, donde se han obtenido buenos resultados después de su aplicación.Palabras clave: Bacillus sphaericus, biocontrolador, biolarvicida, toxina binaria Bin A y Bin B. Abstract Bacillus sphaericus: vector control strategies that produce malaria, yellow fever, and dengue The increased incidence of diseases of public health such as malaria, dengue and yellow fever associated to side effects of the use of chemical pesticides and the resistance generated has prompted the search for new alternatives for the elimination of its vectors. A viable option is the entomopathogenic bacteria Bacillus sphaericus, first described in 1904 by Neide and Meyer who discovered spore-forming bacteria that exists in nature and is easily grown in vitro and in the body of the larvae.Its larvicidal activity is due to the production of a crystalline inclusion, called binary toxin A and B of 41.9 and 51.4 kDa, respectively, which, when released in the gut of susceptible insects in their larval ARTÍCULO DE REVISIÓN

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Electrophysiological Effects ofBacillus sphaericusBinary Toxin on Cultured Mosquito Cells

Cited by 34 publications

References 30 publications

Mosquitocidal bacterial toxins: diversity, mode of action and resistance phenomena

Mosquitocidal bacterial toxins: diversity, mode of action and resistance phenomena

Role of cysteine at positions 67, 161 and 241 of a Bacillus sphaericus binary toxin BinB

Bacillus sphaericus: biocontrolador de vectores que producen malaria, fiebre amarilla y dengue

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