Electroporation and Transgenic Plant Production

Processing of sugar beets assisted by pulsed electric fields

“…Electroporation was then used in many works to introduce a variety of exogenous molecules into sugar beet cells, gene transfer, and it has important applications in agriculture (Hall et al 1994;Joersbo and Brunstedt 1996). Different transformation strategies assisted by electroporation, sonication, somatic hybridisation, and polyethylene glycol (PEG)mediated have been attempted (Gurel et al 2008;Rivera et al 2012;Lal and Lal 2020).…”

The effect of temperature conditions during growth on the transformation frequency of Coccomyxa subellipsoidea C-169 obtained by electroporation

“…Much research in the field of electrotransformation has focused on the optimization of parameters of electric pulses and chemical composition of the media used [ [7] , [8] , [9] ]. The correlation of the cell diameter with the level of electropermeabilization has also been investigated [ 10 , 11 ]. In general, the larger the cells, the more sensitive they are to the applied electric field intensity [ [12] , [13] , [14] ] Usually, there is a positive correlation between the percentage of electropermeabilized cells in a cell population and the increase in the field strength [ 14 , 15 ].…”

“…Many plant protoplast electroporation protocols recommend the use of ice for electroporation. Usually, after electroporation, protoplasts are incubated on ice [ 10 ]. The low temperature prolongs the duration of electro-pores formed on the protoplasts, which leads to increased DNA uptake [ 22 ].…”