Electroseparations, Electrophoresis

Abstract

Electrophoresis is a separation technique which is most often applied to the analysis of biological and other polymeric samples. The principles, modes of operation, separation matrices, detection techniques, and equipment for the various electrophoresis techniques are presented. The three principal modes of electrophoresis, zone electrophoresis, isoelectric focusing, and isotachophoresis, are discussed, as are the advantages of the principal matrices in which electrophoresis is performed, including agarose, polyacrylamide, paper, capillaries, and flowing buffers. Specific examples of techniques such as immunochemical reaction, two‐dimensional techniques, and blotting are given.