Electrospinning Materials for Skin Tissue Engineering

Kınıkoğlu, Beste

doi:10.1002/9781119242628.ch1

Cited by 3 publications

(3 citation statements)

References 70 publications

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“…As a skin regeneration graft, one of the important required characteristics for the matrix is the ability to support cell invasion and infiltration . Thus, we investigated the ability of the gelatin cryogel to support cell infiltration by allowing fibroblasts to grow over and then examining the presence of cells throughout the cross sections of the scaffold (Figure G–I).…”

Section: Results and Discussionmentioning

confidence: 99%

Bilayer Cryogel Wound Dressing and Skin Regeneration Grafts for the Treatment of Acute Skin Wounds

Priya

Gupta

Jain

et al. 2016

ACS Appl. Mater. Interfaces

106

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In this study, the potential of cryogel bilayer wound dressing and skin regenerating graft for the treatment of surgically created full thickness wounds was evaluated. The top layer was composed of polyvinylpyrrolidone-iodine (PVP-I) cryogel and served as the antiseptic layer, while the bottom regenerative layer was made using gelatin cryogel. Both components of the bilayer showed typical features of a cryogel interconnected macropore network, rapid swelling, high water uptake capacity of about 90%. Both PVP and gelatin cryogel showed high tensile strength of 45 and 10 kPa, respectively. Gelatin cryogel sheets were essentially elastic and could be stretched without any visible deformation. The antiseptic PVP-I layer cryogel sheet showed sustained iodine release and suppressed microbial growth when tested with skin pathogens (zone of inhibition ∼2 cm for sheet of 0.9 cm diameter). The gelatin cryogel sheet degraded in vitro in weeks. The gelatin cryogel sheet supported cell infiltration, attachment, and proliferation of fibroblasts and keratinocytes. Microparticles loaded with bioactive molecules (mannose-6-phosphate and human fibrinogen) were also incorporated in the gelatin cryogel sheets for their role in enhancing skin regeneration and scar free wound healing. In vivo evaluation of healing capacity of the bilayer cryogel was checked in rabbits by creating full thickness wound defect (diameter 2 cm). Macroscopic and microscopic observation at regular time intervals for 4 weeks demonstrated better and faster skin regeneration in the wound treated with cryogel bilayer as compared to untreated defect and the repair was comparable to commercial skin regeneration scaffold Neuskin-F. Complete skin regeneration was observed after 4 weeks of implantation with no sign of inflammatory response. Defects implanted with cryogel having mannose-6-phosphate showed no scar formation, while the wound treated with bilayer incorporated with human fibrinogen microparticles showed early signs of skin regeneration; epidermis formation occurred at 2 weeks after implantation.

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Section: Results and Discussionmentioning

confidence: 99%

Bilayer Cryogel Wound Dressing and Skin Regeneration Grafts for the Treatment of Acute Skin Wounds

Priya

Gupta

Jain

et al. 2016

ACS Appl. Mater. Interfaces

106

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“…These two problems impede cell immersion, vascularization, and the integration of the scaffold with the host cell . Electrospinning scaffolds have been widely used in vascular, bone, skin, neutron, and ligament tissue regeneration …”

Section: Introductionmentioning

confidence: 99%

“…42 Electrospinning scaffolds have been widely used in vascular, bone, skin, neutron, and ligament tissue regeneration. [43][44][45] The effect of process variables and material diversity on fiber has been well documented. [46][47][48][49] In this review, we provide an in-depth focus on electrospinning underlining techniques to produce fibers for biological morphology and application.…”

Section: Introductionmentioning

confidence: 99%

Recent studies on electrospinning preparation of patterned, core–shell, and aligned scaffolds

Liu

Mukherjee

Liu

et al. 2018

J of Applied Polymer Sci

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Electrospinning is one of the most important ways to prepare continuous, high porosity, large specific surface area, and uniform diameter micro-and nanoscale fibers. So, it has been widely used in the preparation of micro/nano-sized polymer scaffolds for tissue engineering in recent years. In addition to the versatility in material selection and the processing variables, electrospinning also provides a lot of methods to regulate fiber structure and scaffolds morphology. For example, the near-field electrospinning can provide a method to solve the problem of uncontrollable fiber path; the melt electrospinning eliminates the risk of solvent residue in the construct; the addition of different auxiliary electrodes can make the fiber patterned. This review introduces the underlying principle and characteristics of above electrospinning applied in biomedicine. Herein, we highlight a comprehensive understanding of the technical aspect of this technology for versatile fibers with patterned, core-shell and aligned morphology.

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Electrospun gelatin/methylcellulose hybrid nanofibers promoted the maturation of human cutaneous tissue progenitor cells toward keratinocyte-like cells

et al. 2022

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Electrospinning Materials for Skin Tissue Engineering

Cited by 3 publications

References 70 publications

Bilayer Cryogel Wound Dressing and Skin Regeneration Grafts for the Treatment of Acute Skin Wounds

Bilayer Cryogel Wound Dressing and Skin Regeneration Grafts for the Treatment of Acute Skin Wounds

Recent studies on electrospinning preparation of patterned, core–shell, and aligned scaffolds

Electrospun gelatin/methylcellulose hybrid nanofibers promoted the maturation of human cutaneous tissue progenitor cells toward keratinocyte-like cells

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