Electrosprayed minocycline hydrochloride-loaded microsphere/SAIB hybrid depot for periodontitis treatment

Abstract

Minocycline hydrochloride (MINO) has been one of the most frequently used antibiotics in the treatment of periodontitis due to its antibacterial activity and osteogenesis effects; however, high levels of MINO administered during the treatment halt the formation of new bone. Therefore, the purpose of the present study was to prepare a MINO-microsphere/sucrose acetate isobutyrate (SAIB) hybrid depot to reduce the burst release of MINO and ensure antibacterial and osteogenesis effects of MINO in the treatment of periodontitis. Uniform microspheres, approximately 5 µm size, with a slightly rough surface and different MINO loading (10, 12, and 14%) were prepared, and the microspheres were added into SAIB, after which the burst release significantly decreased from 66.18 to 2.92%, from 71.82 to 3.82%, and from 73.35 to 4.45%, respectively, and the release from all the MINO-microspheres/SAIB hybrid depots lasted for 77 days. In addition, cytotoxicity test showed that the MINO-microsphere with 12% drug loading promoted the proliferation of osteoblasts the most and was subsequently used in vivo experiments. Moreover, in the model of ligatured-induced periodontitis in SD rats, the MINO-microsphere/SAIB hybrid depot not only significantly increased the alveolar bone height and bone volume but also reduced the inflammation of the periodontal tissue. Additionally, it also inhibited the expression of the receptor activator of nuclear factor-kappa B ligand (RANKL) and promoted the expression of osteoprotegerin (OPG).. These results indicated that the MINO-microsphere/SAIB hybrid depot might be promising in the treatment of periodontitis.

The application of electrosprayed minocycline‐loaded PLGA microparticles for the treatment of glioblastoma

“…Solvent ratio Solvents Amount of minocycline (mg) (1082 cm −1 ) bands in PLGA as seen by others (Fu et al, 2017;Zhang et al, 2021). As seen by Zhang et al (2021), the minocycline sample had the characteristic -COOH (1650 and 1600 cm −1 ) and multiple complex peaks associated with the four benzene rings in minocycline (500-1400 cm −1 ). The minocycline-loaded PLGA microparticles had both the PLGA and minocycline associated bands and peaks.…”

“…The absence of additional FTIR peaks in both the minocycline-loaded PLGA microparticles and the residue obtained after evaporation indicates that the interaction between PLGA and minocycline was due to physical blending rather than chemical conjugation (Zhang et al, 2021). When the evaporation collection method was utilized, the size of the particles increased significantly.…”

“…The actual loading efficiency may be even lower as a lot of the drug, as indicated by the burst release in the first hour, may be on the surface of the particles. Zhang et al (2019Zhang et al ( , 2021 incorporated polyethylene glycol (PEG) which a is a hydrophilic material to result in an increase in monodispersity and encapsulation efficiency of their minocycline-loaded microparticles. PLGA containing methyl and methylene hydrophobic groups is not conducive to mixing and encapsulating the hydrophilic drug minocycline.…”

Controlled released naringin-loaded liposome/sucrose acetate isobutyrate hybrid depot for osteogenesis in vitro and in vivo

“…Many studies have reported that SAIB is an efficient material to control the burst release of drugs from sustained release systems ( Lin et al, 2012 ; Lin et al, 2015 ). In our previous studies, we successfully designed an SAIB-based hybrid depot that significantly reduced initial drug release from 66.18% to 2.92% ( Zhang et al, 2021 ) and from 63.20% to .00% ( Zhang et al, 2020 ). Thus, in this study, we first prepared Ng liposomes using the film hydration method and characterized their properties, including their drug-encapsulation efficacy, morphology, size distribution, and drug release.…”

Abalone‐Inspired Adhesive and Photo‐Responsive Microparticle Delivery Systems for Periodontal Drug Therapy

“…[ 2 ] Meanwhile, the surgical process would bring patients fear and discomfort. [ 3 ] As an alternative, antibiotics, such as minocycline, [ 4 ] metronidazole, [ 5 ] and tinidazole, [ 6 ] have shown promising potential in treating periodontitis. [ 7 ] Especially by simply adding these antibiotics to ointments and applying the resultant ointments to the target area, the release of antibiotics would kill the pathogens effectively, bringing about complete inflammatory eradication.…”