Electrospun grape seed polyphenols/gelatin composite fibers contained silver nanoparticles as biomaterials

Abstract

GSP/gelatin composite nanofiber membranes containing silver nanoparticles were successfully fabricated as a novel biomaterial by electrospinning. The silver nanoparticles (AgNPs) were synthesized with the grape seed polyphenols (GSP) as reducing agent in aqueous solution of gelatin, and then the GSP/gelatin/AgNPs mixed solution was electrospun into nanofibers at 55 o C. The scanning electron microscopy (SEM) confirmed that the composite fibers were uniform and the average fiber diameter ranged between 150 nm and 230 nm with an increase in applied potentials from 14 kV to 22 kV. And the transmission electron microscopy (TEM) showed that silver nanoparticles distributed individually in the fibers with the average particle size of about 11 nm. Furthermore, the ultraviolet visible spectrophotometer (UV-vis spectroscopy) test demonstrated that all of Ag + converted to Ag 0 when the concentration of gelatin was 24 wt% and the mass ratio of GSP to AgNO 3 was about 5:2. The antibacterial activities of the fiber membranes against E.coli and S.aureus were measured via a shake flank test and demonstrated good performance after the importation of silver nanopaticles. Cytotoxicity testing also revealed that fiber membranes contained silver nanoparticles had no cyto-toxic. All the results indicated that the GSP was effective for the formation and stabilization of silver nanoparticles in composite nanofibers mats which had the potential for applications in antimicrobial tissue engineering and wound dressing.

Preparation of Nanofibers with Renewable Polymers and Their Application in Wound Dressing

“…In addition to the general properties of a condensed polyphenol, the grape seed polyphenol (GSP) has some unique properties, such as antimicrobial activity, anti-inflammatory activity, and reducing blood sugar and fat. Han et al [69] fabricated GSP/gelatin composite nanofibers containing Ag-NPs by electrospinning as a novel biomaterial. The process is shown in Figure 11.…”

Fabrication of gelatin–chitosan–gum tragacanth with thermal annealing cross-linking strategy

“…Electrospinning is one of the most flexible and simple techniques that allow for the production of fibers with diameters ranging from tens of nanometers to several micrometers. 4,5 The electrospun nanofiber morphology is affected by the material being used and processing parameters. 6 cellulose.…”

“…GT is a complex, heterogeneous, and anionic carbohydrate with prominent structural stability to heat, acidity, and aging. 5,27,28 Tragacanthin, galacturonic acid part of tragacanth which is water soluble and is a neutral, branched with a high molecular weight which gives highly viscous solutions, and bassorin, the other part of tragacanth, is a complex of methoxylated acids that are insoluble in water and swells to form a gel or viscous solution. 8,9 GT has been used as an emulsifier, hydrogel membranes, and pharmaceutical for burn wound healing applications.…”