Electrospun polylactic acid/date palm polyphenol extract nanofibres for tissue engineering applications

Zadeh, Khadija M.; Luyt, Adriaan S.; Zarif, Lubna; Augustine, Robin; Hasan, Anwarul; Messori, Massimo; Hassan, Mohammad K.; Yalcin, Huseyin C.

doi:10.1007/s42247-019-00042-8

Cited by 27 publications

(16 citation statements)

References 37 publications

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“…The strategy of using natural polysaccharides and proteins to reduce free radical production has been explored in wound healing using plant and milk derivatives such as Aloe vera and whey protein [36,37]. Scaffolds loaded with palm extracted polyphenols have shown the ability to reduce free radical-derived inflammation, thus maintaining homeostasis and endothelial cell function [38]. These encouraging results also highlight the increasing scientific interest in the application of characterized natural extracts to biomedicine.…”

Section: Discussionmentioning

confidence: 99%

Waste Autochthonous Tuscan Olive Leaves (Olea europaea var. Olivastra seggianese) as Antioxidant Source for Biomedicine

Ossa

Felice

Azimi

et al. 2019

IJMS

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Olive leaf extract (OLE) can be obtained as biowaste and is extensively used a food supplement and an over-the-counter drug for its beneficial effects. New studies have investigated OLE concerning the role of oxidative stress in the pathogenesis of vascular disease. This in vitro study aims to evaluate if OLE extracted from the Tuscan Olea europaea protects endothelial cells against oxidative stress generated by reactive oxygen species (ROS). Methods: OLE total polyphenols (TPs) were characterized by the Folin–Ciocalteu method. Endothelial cells were grown in conventional cultures (i.e., two-dimensional, 2D) and on a biomaterial scaffold (i.e., three-dimensional, 3D) fabricated via electrospinning. Cell viability and ROS measurement after H2O2 insults were performed. Results: OLE TP content was 23.29 mg GAE/g, and oleuropein was the principal compound. The dose-dependent viability curve highlighted the absence of significant cytotoxic effects at OLE concentrations below 250 µg/mL TPs. By using OLE preconditioning at 100 µg/mL, cell viability decrease was observed, being in 3D lower than in the 2D model. OLE was protective against ROS in both models. Conclusions: OLE represents a high-value antioxidant source obtained by biowaste that is interesting for biomedical products. Using a 3D scaffold could be the best predictive model to mimic the physiological conditions of vascular tissue reaction.

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Section: Discussionmentioning

confidence: 99%

Waste Autochthonous Tuscan Olive Leaves (Olea europaea var. Olivastra seggianese) as Antioxidant Source for Biomedicine

Ossa

Felice

Azimi

et al. 2019

IJMS

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“…On days 4 and 7 of incubation, cell proliferation was much lower for PLA ( w / v )/10 J, PLA ( w / v )/20 J, and PLA ( w / v )/30 J, revealing a clear cytotoxic behavior, as the metabolic activity decreased with more than 50%, compared to the control. Although PLA ( w / v ) samples showed a biocompatible behavior in all 7 days of incubation, with the highest metabolic activity value of 128% (recorded on the seventh day), with the addition of J, the same metabolic activity can be obtained in just one day [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

Fabrication of Electrospun Juglans regia (Juglone) Loaded Poly(lactic acid) Scaffolds as a Potential Wound Dressing Material

et al. 2022

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Juglone (5-hydroxy-1,4-naphthoquinone) (J) is a naphthoquinone structured allelochemical that is mostly found in the roots, leaves, nut-hulls, bark, and wood of walnut (Juglans regia). In this study, the biocompatibility, mechanical, thermal, chemical, morphological, and antimicrobial properties of the poly(lactic acid) (PLA) (w/v)/J (10, 20, 30 mg) electrospun scaffolds were investigated. Based on the results of the study, it was shown that juglone addition increased the antimicrobial properties of the scaffolds against the Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), compared to the neat PLA film after 24 h of contact time. According to the tensile test results, the addition of J made the scaffolds more flexible but decreased the mechanical strength. The cytotoxicity properties of the J-added scaffolds demonstrated a toxic behavior on the first day of incubation. However, with an increase in the J ratio, the fibroblast cell metabolic activity increased for all incubation periods.

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“…This effect could be partly due to increased cell numbers or mitochondrial activities since the XTT assay is generally used to measure cellular metabolic activities as the indicators of cell viability, cytotoxicity, and proliferation [ 39 , 40 , 41 ]. Ascorbic acid and phenolic group have been previously shown to increase cell numbers [ 42 , 43 , 44 , 45 ] and cellular ATP production [ 43 , 46 ]. Additionally, tryptoline derivatives and phenolic derivatives were also able to promote neurite outgrowth [ 47 ], which may be associated with increased mitochondria numbers, accumulated within the neurite [ 48 ].…”

Section: Resultsmentioning

confidence: 99%

“…es 2021, 26, x FOR PEER REVIEW 6 of 23 cell numbers [42][43][44][45] and cellular ATP production [43,46]. Additionally, tryptoline derivatives and phenolic derivatives were also able to promote neurite outgrowth [47], which may be associated with increased mitochondria numbers, accumulated within the neurite [48].…”

Section: Biological Activity Assaysmentioning

confidence: 99%

Novel Multifunctional Ascorbic Triazole Derivatives for Amyloidogenic Pathway Inhibition, Anti-Inflammation, and Neuroprotection

et al. 2021

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Alzheimer’s disease (AD) is a common neurodegenerative disorder. The number of patients with AD is projected to reach 152 million by 2050. Donepezil, rivastigmine, galantamine, and memantine are the only four drugs currently approved by the United States Food and Drug Administration for AD treatment. However, these drugs can only alleviate AD symptoms. Thus, this research focuses on the discovery of novel lead compounds that possess multitarget regulation of AD etiopathology relating to amyloid cascade. The ascorbic acid structure has been designated as a core functional domain due to several characteristics, including antioxidant activities, amyloid aggregation inhibition, and the ability to be transported to the brain and neurons. Multifunctional ascorbic derivatives were synthesized by copper (I)-catalyzed azide–alkyne cycloaddition reaction (click chemistry). The in vitro and cell-based assays showed that compounds 2c and 5c exhibited prominent multifunctional activities as beta-secretase 1 inhibitors, amyloid aggregation inhibitors, and antioxidant, neuroprotectant, and anti-inflammatory agents. Significant changes in activities promoting neuroprotection and anti-inflammation were observed at a considerably low concentration at a nanomolar level. Moreover, an in silico study showed that compounds 2c and 5c were capable of being permeated across the blood–brain barrier by sodium-dependent vitamin C transporter-2.

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Electrospun polylactic acid/date palm polyphenol extract nanofibres for tissue engineering applications

Cited by 27 publications

References 37 publications

Waste Autochthonous Tuscan Olive Leaves (Olea europaea var. Olivastra seggianese) as Antioxidant Source for Biomedicine

Waste Autochthonous Tuscan Olive Leaves (Olea europaea var. Olivastra seggianese) as Antioxidant Source for Biomedicine

Fabrication of Electrospun Juglans regia (Juglone) Loaded Poly(lactic acid) Scaffolds as a Potential Wound Dressing Material

Novel Multifunctional Ascorbic Triazole Derivatives for Amyloidogenic Pathway Inhibition, Anti-Inflammation, and Neuroprotection

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