Electrospun synthetic human elastin:collagen composite scaffolds for dermal tissue engineering

Rnjak‐Kovacina, Jelena; Wise, Steven G.; Li, Zhe; Maitz, Peter; Young, Cara J.; Wang, Yiwei; Weiss, Anthony S.

doi:10.1016/j.actbio.2012.06.032

Cited by 135 publications

(122 citation statements)

References 55 publications

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“…It is conceivable that after day eight of implantation, Integra begins to lose its architecture and mechanical properties, allowing rapid scar contraction [53]. A similar rapid contraction after day eight has been shown in the literature following treatment of full thickness dermal wounds with electrospun collagen scaffolds in a guinea pig model [54]. Together, these data suggest that rapid scar contraction after day eight is related to the longevity of collagen scaffolds in the wound bed, rather than the fabrication technique.…”

Section: Discussionmentioning

confidence: 75%

Mitigation of hypertrophic scar contraction via an elastomeric biodegradable scaffold

et al. 2015

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Section: Discussionmentioning

confidence: 75%

Mitigation of hypertrophic scar contraction via an elastomeric biodegradable scaffold

et al. 2015

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“…It results in nano- and micro-diameter fibers from a protein solution that can be cross-linked to provide structural stability 87 . The mechanical strength, pore size and surface structure of the materials can be controlled by flow rate and concentration of the solution, applied voltage and distance to target 123,124 .…”

Section: Elastin and Tropoelastin: Role In Tissue Repair And Maintmentioning

confidence: 99%

Elastin-based biomaterials and mesenchymal stem cells

et al. 2015

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Elastin is the dominant mammalian elastic protein found in soft tissue. Elastin-based biomaterials have the potential to repair elastic tissues by improving local elasticity and providing appropriate cellular interactions and signaling. Studies that combine these biomaterials with mesenchymal stem cells have demonstrated their capacity to also regenerate non-elastic tissue. Mesenchymal stem cell differentiation can be controlled by their immediate environment, and their sensitivity to elasticity makes them an ideal candidate for combining with elastin-based biomaterials. With the growing accessibility of the elastin precursor, tropoelastin, and elastin-derived materials, the amount of research interest in combining these two fields has increased and, subsequently, is leading to the realization of a potentially new strategy for regenerative medicine.

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“…Young's modulus was calculated as the slope of the stress-strain curve generated over the linear portion of the strain range after the initial toe region. 25 …”

Section: Mechanical Testingmentioning

confidence: 99%

“…Scaffolds were then washed repeatedly in phosphate-buffered saline (PBS). 25 More than half of the 35 free lysines on each tropoelastin molecule react with GA within 2 h, with the optimal balance between crosslinking and scaffold rehydration achieved between 8 and 16 h where scaffolds are composed entirely of tropoelastin. 21 Scanning electron microscopy/fiber quantification Samples for scanning electron microscopy (SEM) analysis were fixed in 2.5% GA in 0.1 M sodium phosphate buffer (PB), postfixed with 1% (v/v) osmium tetraoxide in 0.1 M PB, and dehydrated in ascending grades of ethanol before drying with hexamethyldisilasane.…”

Section: Electrospinningmentioning

confidence: 99%

“…21 Incorporated in scaffolds, human tropoelastin promotes cell proliferation 22 and blends with the relatively stiff PLGA 23 and PCL to confer enhanced growth of endothelial cells, increased elasticity, and improved blood compatibility, 24 while tropoelastin/collagen hybrids show significantly improved performance for dermal scaffolding applications. 25 We reasoned that a coblend of polyurethane Elast-Eon E2A (denoted as E2A) and tropoelastin would uniquely result in a scaffold combining strength, elasticity, and cell interactivity. In this study, we present a new class of elastic hybrids comprising blended E2A polyurethane and recombinant human tropoelastin, with enhanced elasticity and fibroblast interactions over E2A alone.…”

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confidence: 99%

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Blended Polyurethane and Tropoelastin as a Novel Class of Biologically Interactive Elastomer

Wise

Liu

Yeo

et al. 2016

Tissue Engineering Part A

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Polyurethanes are versatile elastomers but suffer from biological limitations such as poor control over cell attachment and the associated disadvantages of increased fibrosis. We address this problem by presenting a novel strategy that retains elasticity while modulating biological performance. We describe a new biomaterial that comprises a blend of synthetic and natural elastomers: the biostable polyurethane Elast-Eon and the recombinant human tropoelastin protein. We demonstrate that the hybrid constructs yield a class of coblended elastomers with unique physical properties. Hybrid constructs displayed higher elasticity and linear stress-strain responses over more than threefold strain. The hybrid materials showed increased overall porosity and swelling in comparison to polyurethane alone, facilitating enhanced cellular interactions. In vitro, human dermal fibroblasts showed enhanced proliferation, while in vivo, following subcutaneous implantation in mice, hybrid scaffolds displayed a reduced fibrotic response and tunable degradation rate. To our knowledge, this is the first example of a blend of synthetic and natural elastomers and is a promising approach for generating tailored bioactive scaffolds for tissue repair.

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Electrospun synthetic human elastin:collagen composite scaffolds for dermal tissue engineering

Cited by 135 publications

References 55 publications

Mitigation of hypertrophic scar contraction via an elastomeric biodegradable scaffold

Mitigation of hypertrophic scar contraction via an elastomeric biodegradable scaffold

Elastin-based biomaterials and mesenchymal stem cells

Blended Polyurethane and Tropoelastin as a Novel Class of Biologically Interactive Elastomer

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