Electrostatic-Spray Ionization Mass Spectrometry

Qiao, Liang; Sartor, Romain; Gasilova, Natalia; Lu, Yu; Tobolkina, Elena; Liu, Baohong; Girault, Hubert H.

doi:10.1021/ac301332k

Cited by 69 publications

(99 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…is process is used to generate high voltage pulses for onsetting a spray from a liquid droplet placed over poly (methylmethacrylate) substrate. 76) e principle of this contactless spray was proposed from a capacitive coupling e ect. is technique termed as electrostatic-spray ionization has been used for analysis of adsorbed proteins, 77) perfumes, 78) and imaging applications.…”

Section: Polarizationmentioning

confidence: 99%

Electrospray Modifications for Advancing Mass Spectrometric Analysis

Meher

Chen

2017

Mass Spectrometry

View full text Add to dashboard Cite

Generation of analyte ions in gas phase is a primary requirement for mass spectrometric analysis. One of the ionization techniques that can be used to generate gas phase ions is electrospray ionization (ESI). ESI is a so ionization method that can be used to analyze analytes ranging from small organics to large biomolecules. Numerous ionization techniques derived from ESI have been reported in the past two decades.ese ion sources are aimed to achieve simplicity and ease of operation. Many of these ionization methods allow the exibility for elimination or minimization of sample preparation steps prior to mass spectrometric analysis. Such ion sources have opened up new possibilities for taking scienti c challenges, which might be limited by the conventional ESI technique. us, the number of ESI variants continues to increase. is review provides an overview of ionization techniques based on the use of electrospray reported in recent years. Also, a brief discussion on the instrumentation, underlying processes, and selected applications is also presented.

show abstract

Section: Polarizationmentioning

confidence: 99%

Electrospray Modifications for Advancing Mass Spectrometric Analysis

Meher

Chen

2017

Mass Spectrometry

View full text Add to dashboard Cite

show abstract

“…19). This result was obtained by analyzing a droplet of a 2 mM L-DOPA solution over a tyrosinase-free PVDF membrane by using the recently developed electrostatic spray ionization (ESTASI) MS (for more details see SI-4) [43]. In contrast, when the droplet of L-DOPA solution was placed over a tyrosinase-bound PVDF region, the peak of L-DOPA disappeared rapidly after only 2 min due to the enzymatic reaction between L-DOPA and tyrosinase (see Fig.…”

Section: Differential Pulse Voltammetry (Dpv) Measurementsmentioning

confidence: 99%

Multiple scanning electrochemical microscopy mapping of tyrosinase in micro-contact printed fruit samples on polyvinylidene fluoride membrane

Lin

Cortés‐Salazar

Lesch

et al. 2015

Electrochimica Acta

Self Cite

View full text Add to dashboard Cite

Keywords:Scanning electrochemical microscopy tyrosinase enzymatic activity electrochemical immunoassay readout A B S T R A C T Herein, we introduce three orthogonal and compatible methods for detecting tyrosinase, a key factor in fruit browning and skin disorders, with high spatial resolution by means of scanning electrochemical microscopy (SECM). All methods are performed subsequently on the same substrate area providing a wide range of relevant information. The first SECM approach that relies on the mapping of a differential pore oxygen concentration induced by the local hydrophobic changes that the adsorption of tyrosinase generates on a porous polyvinylidene fluoride (PVDF) membrane. The second approach is based on the direct monitoring of the enzymatic activity of tyrosinase by detecting amperometrically the reaction products from the enzymatic conversion of L-3,4-dihydroxyphenylalanine (L-DOPA). Finally, tyrosinase was visualized implementing a tyrosinase sandwich immunoassay readout by SECM. The multiple SECM detection strategies were successfully applied to map unequivocally the tyrosinase enzymatic activity of a micro-contact printed banana sample. Furthermore, differential pulse voltammetry and mass spectrometry analyses were employed to elucidate the nature of the electrochemical response obtained during the tyrosinase enzymatic activity experiments.

show abstract

“…Peptides and antibiotics could be separated by capillary electrophoresis, where the fraction was collected and dried on a plastic plate to form arrays of sample spots. For the analysis, an acidic solution was added on each spot followed immediately by ESTASI-MS. [19,23] The peptides or proteins separated by isoelectric focusing (IEF) in polyacrylamide gels can also be detected in situ by ESTASI-MS. [24] By adding a droplet of acidic solution on top of the sample band, protons could diffuse into the gel and protonate the peptides or proteins inside. Afterwards, by applying the HV, the positively charged sample was extracted from the gel into the droplet for ESTASI-MS analysis.…”

Section: Scanning Electrochemical Microscopymentioning

confidence: 99%

“…[18] Electrostatic spray ionization (ESTASI) is an ambient ionization method, which has been developed by LEPA since 2012 and which is characterized by inducing the spray by electrostatic forces in order to reduce significantly the influence of the applied high voltage (HV) on the sample. [19] The ESTASI device is illustrated in Fig. 2 showing the electrode connected to the HV, an insulator isolating the sample from the electrode and a counterelectrode that is normally the MS inlet.…”

Section: Electrostatic Spray Ionization Mass Spectrometrymentioning

confidence: 99%

Analytical Chemistry at the Laboratoire d'Electrochimie Physique et Analytique

Bondarenko

Cortés‐Salazar

Gasilova

et al. 2015

Chimia

Self Cite

View full text Add to dashboard Cite

The Laboratoire d'Electrochimie Physique et Analytique (LEPA) has moved to the new Energypolis campus in Sion. This laboratory is involved in energy research in particular by studying charge transfer reactions at soft interfaces and developing interfacial redox electrocatalysis, by pioneering the concept of photo-ionic cells and by integrating redox flow batteries for the production of hydrogen at the pilot scale. Nonetheless, this laboratory has a long tradition in analytical chemistry with the development of microfabrication techniques such as laser photo-ablation, screen-printing and more recently inkjet printing for the design and fabrication of biosensors and immunosensors. As shown in the present review, the laboratory has recently pioneered new technologies for electrochemical and mass spectrometry imaging and for the screening of allergy in patients. The role of the laboratory in the Valais landscape will be to foster the collaboration with the HES to develop teaching and research in analytical chemistry as this field is a major source of employment for chemists.

show abstract

Electrostatic-Spray Ionization Mass Spectrometry

Cited by 69 publications

References 24 publications

Electrospray Modifications for Advancing Mass Spectrometric Analysis

Electrospray Modifications for Advancing Mass Spectrometric Analysis

Multiple scanning electrochemical microscopy mapping of tyrosinase in micro-contact printed fruit samples on polyvinylidene fluoride membrane

Analytical Chemistry at the Laboratoire d'Electrochimie Physique et Analytique

Contact Info

Product

Resources

About