Electrostatic‐spray ionization mass spectrometry sniffing for perfume fingerprinting

Abstract

RATIONALE:The perfume market is growing significantly, and it is easy to find imitative fragrances of probably all types of perfume. Such imitative fragrances are usually of lower quality than the authentic ones, creating a possible threat for perfume companies. Therefore, it is important to develop efficient chemical analysis techniques to screen rapidly perfume samples. METHODS: Electrostatic-spray ionization (ESTASI) was used to analyze directly samples sprayed or deposited on different types of paper. A linear ion trap mass spectrometer was used to detect the ions produced by ESTASI with a modified extended transfer capillary for 'sniffing' ions from the paper. RESULTS: Several commercial perfumes and a model perfume were analyzed by ESTASI-sniffing. The results obtained by paper ESTASI-MS of commercial fragrances were compared with those obtained from ESI-MS. In addition, a commercial fragrance was first nebulized on the hand and then soaked up by blotting paper, which was afterwards placed on an insulating plate for ESTASI-MS analysis. Analysis of peptides and proteins was also performed to show that the paper ESTASI-MS could be used for samples with very different molecular masses. CONCLUSIONS: Paper ESTASI-MS yields a rapid fingerprinting characterization of perfume fragrances, avoiding timeconsuming sample-preparation steps, and thereby performing a rapid screening in a few seconds.

Fingerprinting the tertiary structure of electroadsorbed lysozyme at soft interfaces by electrostatic spray ionization mass spectrometry

“…4 After pre-concentration of lysozyme at the gelled ITIES, the organogel was cut from the end of the glass pipette and fixed onto a 0.2 mm thick insulating plastic substrate (GelBond s PAG, Lonza). For ESTASI-MS analysis, the organogel was placed close to the MS inlet (''L'' shaped ion transfer capillary) 14 and a gold electrode was positioned underneath the insulating plastic substrate. Drops (1-2 mL) of an acidic solution [1% (v/v) acetic acid in 50 : 50 water : methanol (v : v)] were placed on top of the organogel.…”

Electrospray Modifications for Advancing Mass Spectrometric Analysis

“…76) e principle of this contactless spray was proposed from a capacitive coupling e ect. is technique termed as electrostatic-spray ionization has been used for analysis of adsorbed proteins, 77) perfumes, 78) and imaging applications.…”

Electrostatic Spray Ionization from 384-Well Microtiter Plates for Mass Spectrometry Analysis-Based Enzyme Assay and Drug Metabolism Screening

“…ESTASI-MS was performed as described in previous publications , and illustrated by Figure . A 384-well plate was placed on a PET substrate (120 μm thickness), and all together between an electrode and the ion transfer capillary of a linear ion trap mass spectrometer (LTQ Velos, Thermo scientific).…”