Elevated gaseous luminal nitric oxide and circulating IL-8 as features of Helicobacter pylori-induced gastric inflammation

Saaed, Hiwa; Chiggiato, Lisa; Webb, Dominic-Luc; Rehnberg, Ann-Sofie; Rubio, Carlos A.; Befrits, Ragnar; Hellström, Per M.

doi:10.48101/ujms.v126.8116

Cited by 8 publications

(9 citation statements)

References 40 publications

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“…EDTA-plasma was separated from whole blood and immediately frozen at -80° C until assay. Following a signed sample transfer agreement approved by the national authority and following standard sample transportation procedures, specimens were sent to the Department of Medical Sciences, Gastroenterology and Hepatology Unit, Uppsala University, Sweden ( 17 ), for immunological assays. The same procedures were followed with the participants in the healthy control group, where 5 ml of blood was collected, and their sociodemographic and health-related data collected following their written consent.…”

Section: Methodsmentioning

confidence: 99%

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Inflammatory mediators profile in patients hospitalized with COVID-19: A comparative study

et al. 2022

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Abnormal inflammatory mediator concentrations during SARS-CoV-2 infection may represent disease severity. We aimed to assess plasma inflammatory mediator concentrations in patients with SARS-CoV-2 in Addis Ababa, Ethiopia. In this study, 260 adults: 126 hospitalized patients with confirmed COVID-19 sorted into severity groups: severe (n=68) and mild or moderate (n=58), and 134 healthy controls were enrolled. We quantified 39 plasma inflammatory mediators using multiplex ELISA. Spearman rank correlation and Mann-Whitney U test were used to identify mechanistically coupled inflammatory mediators and compare disease severity. Compared to healthy controls, patients with COVID-19 had significantly higher levels of interleukins 1α, 2, 6, 7, 8, 10 and 15, C-reactive protein (CRP), serum amyloid A (SAA), intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion protein 1 (VCAM-1), IFN-γ-inducible protein-10 (IP-10, CXCL10), macrophage inflammatory protein-1 alpha (MIP-1α, CCL3), eotaxin-3 (CCL26), interferon-gamma (IFN-γ), tumor necrosis factor-α (TNF-α), basic fibroblast growth factor (bFGF), placental growth factor (PlGF), and fms-like tyrosine kinase 1 (Flt-1). Patients with severe COVID-19 had higher IL-10 and lower macrophage-derived chemokine (MDC, CCL22) compared to the mild or moderate group (P<0.05). In the receiver operating characteristic curve, SAA, IL-6 and CRP showed strong sensitivity and specificity in predicting the severity and prognosis of COVID-19. Greater age and higher CRP had a significant association with disease severity (P<0.05). Our findings reveal that CRP, SAA, VCAM-1, CXCL10, CCL22 and IL-10 levels are promising biomarkers for COVID-19 disease severity, suggesting that plasma inflammatory mediators could be used as warning indicators of COVID-19 severity, aid in COVID-19 prognosis and treatment.

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Section: Methodsmentioning

confidence: 99%

“…Testings were performed at the Department of Medical Sciences, Gastroenterology and Hepatology Unit, Uppsala University, Sweden ( 17 ).…”

Section: Methodsmentioning

confidence: 99%

Inflammatory mediators profile in patients hospitalized with COVID-19: A comparative study

et al. 2022

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“…A human cytokine 39-plex ELISA assay was used (Meso Scale Diagnostics, Rockville, MD, USA) to measure basic fibroblast growth factor (bFGF); C-reactive protein (CRP); eosinophil chemotactic proteins (Eotaxin, Eotaxin-3); fms-like tyrosine kinase 1 (Flt-1); granulocyte-macrophage colony-stimulating factor (GM-CSF); intercellular adhesion molecule 1 (ICAM-1); interferons (IFN-γ); IFN-γ-inducible protein-10 (IP-10); interleukins (IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12p70, IL-12/IL-23p40, IL-13, IL-15, IL-16, IL-17A); macrophage-derived chemokine (MDC); macrophage-inflammatory protein (MIP-1α, MIP-1β); monocyte chemoattractant protein (MCP-1, MCP-4), placental growth factor (PIGF); serum amyloid A (SAA); thymus activation-regulated chemokine (TARC); tumor necrosis factors (TNF-α, TNF-β); tyrosine-protein kinase receptor (Tie-2); vascular cell adhesion protein 1 (VCAM-1); vascular endothelial growth factor (VEGF-A, VEGF-C, VEGF-D) at Department of Medical Sciences, Gastroenterology and Hepatology Unit, Uppsala University, Sweden (14).…”

Section: Methodsmentioning

confidence: 99%

Cytokine and chemokine profile in patients hospitalized with COVID-19: A comparative study

Bedada

2022

Preprint

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Abnormal cytokine and chemokine concentrations during SARS-CoV-2 infection may represent disease severity. We aimed to assess plasma cytokine and chemokine concentrations in patients with SARS-CoV-2 in Addis Ababa, Ethiopia. In this study, 260 adults: 126 hospitalized patients with confirmed COVID-19 sorted into severity groups: severe (n=68) and mild or moderate (n=58), and 134 healthy controls were enrolled. We quantified 39 plasma cytokines and chemokines using multiplex ELISA. Spearman rank correlation and Mann-Whitney U test were used to identify mechanistically coupled cytokines/chemokines and compare disease severity. Compared to healthy controls, patients with COVID-19 had significantly higher levels of interleukins 1α, 2, 6, 7, 8, 10 and 15, C-reactive protein (CRP), serum amyloid A (SAA), intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion protein 1 (VCAM-1), IFN-γ-inducible protein-10 (IP-10), macrophage inflammatory protein-1 alpha (MIP-1α), eotaxin-3, interferon-gamma (IFN-γ), tumor necrosis factor-α (TNF-α), basic fibroblast growth factor (bFGF), placental growth factor (PlGF), and fms-like tyrosine kinase 1 (Flt-1). Patients with severe COVID-19 had higher IL-10 and lower macrophage-derived chemokine (MDC) compared to the mild or moderate group (P<0.05). In the receiver operating characteristic curve, SAA, IL-6 and CRP showed strong sensitivity and specificity predicting the severity and prognosis of COVID-19. Greater age and higher CRP had a significant association with disease severity (P<0.05). Our findings reveal that CRP, SAA, VCAM-1, IP-10, MDC and IL-10 levels are promising biomarkers for COVID-19 disease severity, suggesting that plasma cytokines/chemokines could be used as warning indicators of COVID-19 severity, aid in COVID-19 prognosis and treatment.

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“…The third calcium-independent isoform, iNOS, is expressed in response to inflammatory cytokines and bacterial virulence factors inducing the release of NO [ 40 , 71 ]. Over-production of NO triggers the pathogenesis of a variety of diseases including gastritis, and upregulation of iNOS levels has been observed in gastritis patients with H. pylori infection [ 72 , 73 ]. Interestingly, Nam et al showed that iNOS levels were significantly diminished after inhibition and eradication of H. pylori [ 74 ].…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Effect of Korean Propolis on Helicobacter pylori-Infected Gastric Mucosal Injury Mice Model

et al. 2022

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Propolis, a natural resinous substance obtained from a variety of buds and plants, has been reported to possess various biological functions. Several recent studies have demonstrated the inhibitory effects of propolis on the growth of Helicobacter pylori (H. pylori) in vitro; however, current research efforts on Korean propolis (KP) remain insufficient especially in vivo. Our study aims to investigate the anti-inflammatory effect and molecular mechanism of KP on mouse gastric mucosa during H. pylori infection. We examined an in vivo H. pylori-induced gastric mucosal injury mice model. We found that KP inhibited the growth of H. pylori and attenuated the expression of H. pylori virulence factors such as cytotoxin-associated gene A, encoding urease A subunit, surface antigen gene and neutrophil-activating protein A. Moreover, KP reduced both gross lesions and pathological scores in H. pylori-challenged mice. In addition, KP markedly restrained the production of pro-inflammatory cytokines and nitric oxide levels compared with an untreated H. pylori-infected group. In particular, we found that KP repressed the phosphorylation of IκBα and NF-κB p65 subunit, and subsequently suppressed their downstream target genes. Taken together, these findings demonstrate the beneficial effects of KP on inflammation through the inhibition of NF-κB signaling as well as inhibition of H. pylori growth in a mouse model infected with H. pylori. This suggests the potential application of KP as a natural supplement for patient’s suffering from gastric mucosal injury caused by H. pylori infection.

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Elevated gaseous luminal nitric oxide and circulating IL-8 as features of Helicobacter pylori-induced gastric inflammation

Cited by 8 publications

References 40 publications

Inflammatory mediators profile in patients hospitalized with COVID-19: A comparative study

Inflammatory mediators profile in patients hospitalized with COVID-19: A comparative study

Cytokine and chemokine profile in patients hospitalized with COVID-19: A comparative study

Anti-Inflammatory Effect of Korean Propolis on Helicobacter pylori-Infected Gastric Mucosal Injury Mice Model

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