Elevated Lin28B expression is correlated with lymph node metastasis in oral squamous cell carcinomas

Lin, Wei-Tung; Shieh, Tzong‐Ming; Yang, Li‐Chiu; Wang, Tung-Yuan; Chou, Ming‐Yung; Yu, Cheng–Chia

doi:10.1111/jop.12314

Cited by 15 publications

(17 citation statements)

References 32 publications

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“…LIN28B protein was found to be elevated in colon tumors, and its overexpression correlated with decreased patient survival and increased tumor recurrence . Further studies also found that the overexpression of LIN28B was associated with poor prognosis in head and neck cancer, gastric cancer, oral squamous cell carcinoma patients, breast cancer, ovarian cancer, and neuroblastoma . In cases of pediatric cancers, such as germ cell tumors, neuroblastoma and WT, LIN28B appears to prolong the embryonic patterns of tissue growth .…”

Section: Discussionmentioning

confidence: 92%

The correlation between LIN28B gene potentially functional variants and Wilms tumor susceptibility in Chinese children

Liu

Zhang

et al. 2017

Clinical Laboratory Analysis

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Section: Discussionmentioning

confidence: 92%

The correlation between LIN28B gene potentially functional variants and Wilms tumor susceptibility in Chinese children

Liu

Zhang

et al. 2017

Clinical Laboratory Analysis

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“…We reasoned that this discrepancy might be due to inherent tumor heterogeneity and different immunohistochemical scores and patient stratification. Interestingly, when the manuscript was in preparation, Lin and his colleague reported that elevated LIN28B correlated with lymph node metastasis in OSCC, although the mRNA abundance of LIN28B was utilized as readout by quantitative real-time PCR assay in 20 paired samples [ 32 ]. Therefore, our findings together with others strongly support the notion that LIN28B is aberrantly overexpressed in OSCC and significantly correlated with malignant clinicopathological features.…”

Section: Discussionmentioning

confidence: 99%

The pluripotency factor LIN28B is involved in oral carcinogenesis and associates with tumor aggressiveness and unfavorable prognosis

et al. 2015

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ObjectiveLIN28B is a conserved RNA-binding protein critically involved in development, cellular metabolism and tumorigenesis. It is frequently overexpressed in human cancers and correlates with tumor aggressiveness as well as unfavorable prognosis. However, the expression pattern and oncogenic roles of LIN28B during oral squamous cell carcinoma (OSCC) development and progression has not been well established yet. Here, we sought to determine the expression of LIN28B and its clinical significance using chemical-induced OSCC animal model, cell lines and primary specimens.MethodThe OSCC animal model was induced using 7,12-dimethyl-1,2-bezan-tracene (DMBA) painting in the hamster buccal pouch. Buccal lesions from animals were obtained from different time points and subjected to routine histological analyses and immunohistochemical staining of LIN28B. The mRNA, protein abundance and subcellular localization of LIN28B was determined in a panel of OSCC cell lines by real-time RT-PCR, western blot and immunofluorescence. The expression levels of LIN28B in human primary OSCC samples were further evaluated by immunohistochemical staining. Moreover, the relationship between LIN28B and several clinicopathological parameters as well as patients’ prognosis were also assessed.ResultsOur results revealed that negative or low LIN28B expression was commonly observed in normal epithelial, whereas more LIN28B abundance was identified in epithelial dysplasia and invasive SCC in the DMBA-induced OSCC animal model. Overexpression of LIN28B was identified in a major fraction of OSCC samples(39/58) and significantly associated with tumor size (P = 0.049) and advanced clinical stages (P = 0.0286). Patients with increased LIN28B had markedly reduced overall survival as compared to those with low LIN28B. Multivariate survival analyses further indicated that LIN28B abundance served as an independent prognostic factor for patients’ overall survival.ConclusionsOur findings reveal that LIN28B is critically involved in OSCC initiation and progression and aberrantly overexpressed in human OSCC. It might represent a novel diagnostic and prognostic biomarker for oral cancer.

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“…Tumor tissues were microscopically screened to have >70% of their areas occupied by tumor cells; The remaining specimen (tumor, normal counterpart, and lymph node metastatic lesions) were snap frozen in liquid nitrogen and stored at −80°C for HOTAIR analysis. Six OSCC cell lines, including Fadu (hypopharyngeal squamous cell carcinoma carcinoma) [ 50 ], OECM-1 (gingival squamous cell carcinoma cells) [ 51 ], GNM (neck metastasis of gingival carcinoma) [ 52 ], Ca9-22 (gingival carcinoma) [ 53 ], SCC4 (tongue squamous cell carcinoma), HSC3 (tongue squamous cell carcinoma) cells [ 53 ] and primary normal human oral keratinocytes (NHOK) cells have been reported and were used in this study [ 5 ]. Briefly, Fadu and OECM-1 cells were in RPMI supplemented with 10% fetal bovine serum (FBS; Life Technologies, Carlsbad, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Targeting LncRNA HOTAIR suppresses cancer stemness and metastasis in oral carcinomas stem cells through modulation of EMT

Liao

Chen

et al. 2017

Oncotarget

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Increasing evidence indicates that long non-coding RNAs (lncRNAs) regulate diverse cellular processes, such as cell growth, apoptosis and tumorigenesis. However, the functional roles of lncRNAs and mechanistic analysis of their interplays with oncogenic pathways in oral cancer remain largely unknown. In the current study, we examined the significance of lncRNA HOTAIR (HOX transcript antisense RNA) in tumor progression of oral squamous cell carcinomas (OSCC). We found the expression of HOTAIR was upregulated in tumor tissues, especially in the metastatic samples. And it was also observed in metastatic OSCC cell lines. Silence of HOTAIR in oral carcinomas stem cells (OCSC) significantly inhibited their cancer stemness, invasiveness and tumourigenicity in xenotransplantation models. By contrast, overexpression of HOTAIR in OSCC enhanced their metastatic potential and epithelial-mesenchymal transition (EMT) characteristics. And we showed that the expression of HOTAIR was positively related to mesenchymal markers and inversely correlated with epithelial marker in clinical samples. Moreover, Kaplan-Meier survival analysis suggested that high level of HOTAIR was a strong predictor of poor survival in OSCC patients. Collectively, our data demonstrated that HOTAIR-mediated cancer stemness and metastasis are associated with the regulation of EMT and HOTAIR may serve as a therapeutic target in OSCC.

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Elevated Lin28B expression is correlated with lymph node metastasis in oral squamous cell carcinomas

Abstract: Lin28B may be a useful biomarker and novel molecular target for LN metastasis OSCC patients' treatment.

Cited by 15 publications

References 32 publications

The correlation between LIN28B gene potentially functional variants and Wilms tumor susceptibility in Chinese children

The correlation between LIN28B gene potentially functional variants and Wilms tumor susceptibility in Chinese children

The pluripotency factor LIN28B is involved in oral carcinogenesis and associates with tumor aggressiveness and unfavorable prognosis

Targeting LncRNA HOTAIR suppresses cancer stemness and metastasis in oral carcinomas stem cells through modulation of EMT

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