Elevated serum IgG titer and avidity to <i>Actinobacillus actinomycetemcomitans</i> serotype c in Japanese periodontitis patients

Wang, D.; Kawashima, Yoko; Nagasawa, Toshiyuki; Takeuchi, Yasuo; Kojima, Toshihiko; Umeda, Makoto; Oda, Shigeru; Ishikawa, Isao

doi:10.1111/j.1399-302x.2005.00208.x

Cited by 26 publications

(32 citation statements)

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“…Interestingly, both A. actinomycetemcomitans and P. gingivalis are capable of inducing strong antibody responses exceeding those found in many other bacterial infections [11]. Elevated levels of circulating antibodies against A. actinomycetemcomitans and P. gingivalis in patients with periodontitis have often been reported [12–16]; and in a large population of adults, who participated in the third US National Health and Nutrition Examination Survey, high titers of antibodies against P. gingivalis were consistently associated with periodontitis [17]. …”

Section: Introductionmentioning

confidence: 99%

Immunoglobulin G antibodies againstPorphyromonas gingivalisorAggregatibacter actinomycetemcomitansin cardiovascular disease and periodontitis

Damgaard

Reinholdt

Enevold

et al. 2017

Journal of Oral Microbiology

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Objectives: The aim was to elucidate whether levels of circulating antibodies to Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis correlate to loss of attachment, as a marker for periodontitis and cardiovascular disease (CVD). Design: Sera were collected from 576 participants of the Danish Health Examination Survey (DANHES). Immunoglobulin G antibodies against lipopolysaccharide (LPS) and protein antigens from the a, b and c serotypes of A. actinomycetemcomitans and P. gingivalis were quantified by titration in ELISA plates coated with a mixture of antigens prepared by disintegration of bacteria. Results: Levels of antibodies against P. gingivalis (OR = 1.48) and A. actinomycetemcomitans (1.31) associated with periodontitis, as determined by univariable logistic regression analysis. These antibody levels also associated with CVD (1.17 and 1.37), respectively, However, after adjusting for other risk factors, including age, smoking, gender, alcohol consumption, overweight, and level of education using multivariable logistic regression analysis, only increasing body mass index (BMI; 1.09), previous smoking (1.99), and increasing age (decades) (2.27) remained associated with CVD. Increased levels of antibodies against P. gingivalis (1.34) remained associated with periodontitis after adjusting for other risk factors. Conclusions: CVD and periodontitis were associated with levels of IgG antibodies to P. gingivalis or A. actinomycetemcomitans in univariable analyses, but only the association of P. gingivalis antibody levels with periodontitis reached statistical significance after adjustment for common confounders. Age, in particular, influenced this relationship.

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Section: Introductionmentioning

confidence: 99%

Immunoglobulin G antibodies againstPorphyromonas gingivalisorAggregatibacter actinomycetemcomitansin cardiovascular disease and periodontitis

Damgaard

Reinholdt

Enevold

et al. 2017

Journal of Oral Microbiology

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“…Aggregatibacter actinomycetemcomitans ATCC 43718 and Porphyromonas gingivalis 381 were cultured, and sonicated extracts were prepared as described previously [41]. Live P. gingivalis 381 (kindly gifted from Dr. Takeuchi), P. gingivalis LPS (InvivoGen, San Diego, CA), E. coli 055:B5 LPS (Sigma, Taufkirchen, Germany), IL-6, IFN-β, and IFN-γ (R&D systems, Minneapolis, MN) were purchased from the respective companies.…”

Section: Methodsmentioning

confidence: 99%

Modulation of Wnt5a Expression by Periodontopathic Bacteria

et al. 2012

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Wingless proteins, termed Wnt, are involved in embryonic development, blood cell differentiation, and tumorigenesis. In mammalian hematopoiesis, Wnt signaling is essential for stem-cell homeostasis and lymphocyte differentiation. Recent studies have suggested that these molecules are associated with cardiovascular diseases, rheumatoid arthritis, and osteoarthritis. Furthermore, Wnt5a signaling is essential for the general inflammatory response of human macrophages. Periodontitis is a chronic inflammatory disease caused by gram-negative periodontopathic bacteria and the resultant host immune response. Periodontitis is characterized by loss of tooth-supporting structures and alveolar bone resorption. There have been no previous reports on Wnt5a expression in periodontitis tissue, and only few study reported the molecular mechanisms of Wnt5a expression in LPS-stimulated monocytic cells. Using RT-PCR, we demonstrated that Wnt5a mRNA expression was up-regulated in chronic periodontitis tissue as compared to healthy control tissue. P. gingivalis LPS induced Wnt5a mRNA in the human monocytic cell line THP-1 with a peak at 4 hrs after stimulation. P. gingivalis LPS induced higher up-regulation of Wnt5a mRNA than E. coli LPS. The LPS receptors TLR2 and TLR4 were equally expressed on the surface of THP-1 cells. P. gingivalis LPS induced IκBα degradation and was able to increase the NF-κB binding activity to DNA. P. gingivalis LPS-induced Wnt5a expression was inhibited by NF-κB inhibitors, suggesting NF-κB involvement. Furthermore, IFN-γ synergistically enhanced the P. gingivalis LPS-induced production of Wnt5a. Pharmacological investigation and siRNA experiments showed that STAT1 was important for P. gingivalis LPS-induced Wnt5a expression. These results suggest that the modulation of Wnt5a expression by P. gingivalis may play an important role in the periodontal inflammatory process and serve a target for the development of new therapies.

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“…Microbiological evaluation : The results of PCR analysis of oral and blood samples for all subjects are shown in Table IV. P. gingivalis, T. forsythensis, and T. (67) 10 (77) 12 (92) 11 (85) 0 7 (54) 0 0 4 (27) 1 (7) 0 0 0 2 (15) 0 0 CHD indicates coronary heart disease; ACS, acute coronary syndrome; PCR, polymerase chain reaction. * ; P < 0.05.…”

Section: Characteristics Of the Study Populationmentioning

confidence: 99%

High Incidence of Actinobacillus Actinomycetemcomitans Infection in Acute Coronary Syndrome

et al. 2007

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SUMMARYRecent epidemiological studies suggest that periodontitis is an important risk factor for coronary heart disease (CHD). The aim of this study was to evaluate the association between periodontitis and CHD, particularly acute coronary syndrome (ACS), focusing on microbiological and immunological features.Twenty-eight CHD patients, 15 with ACS and 13 with chronic CHD, were included in this study. Coronary angiography, periodontal examination, and dental radiography were performed in all patients. Subgingival plaque, saliva, and blood samples were analyzed for the periodontopathogens Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythensis, Treponema denticola, and Prevotella intermedia using polymerase chain reaction.Specific serum antibody titers to the 5 periodontal pathogens were determined by enzyme-linked immunosorbent assay. It was found that 33% of the ACS patients (5/15) harbored A. actinomycetemcomitans in oral samples, whereas no A. actinomycetemcomitans (0/13) was found in the chronic CHD patients (P < 0.05). Furthermore, ACS patients showed significantly higher serum IgG titers to A. actinomycetemcomitans (P < 0.05) compared with chronic CHD. More tooth loss and alveolar bone loss were noted in ACS patients than in chronic CHD patients, although the differences were not statistically significant.Periodontal pathogens, particularly A. actinomycetemcomitans, may play a role in the development of ACS. (Int Heart J 2007; 48: 663-675)

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Elevated serum IgG titer and avidity to Actinobacillus actinomycetemcomitans serotype c in Japanese periodontitis patients

Abstract: A. actinomycetemcomitans serotype c may play a significant role in chronic and generalized aggressive periodontitis, while A. actinomycetemcomitans serotype b may be associated with localized aggressive periodontitis in a Japanese population.

Cited by 26 publications

References 47 publications

Immunoglobulin G antibodies againstPorphyromonas gingivalisorAggregatibacter actinomycetemcomitansin cardiovascular disease and periodontitis

Immunoglobulin G antibodies againstPorphyromonas gingivalisorAggregatibacter actinomycetemcomitansin cardiovascular disease and periodontitis

Modulation of Wnt5a Expression by Periodontopathic Bacteria

High Incidence of Actinobacillus Actinomycetemcomitans Infection in Acute Coronary Syndrome

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