Eliminating Mosquitoes with Precision Guided Sterile Males

Li, Ming; Yang, Ting; Bui, Michelle; Gamez, Stephanie; Wise, Tyler; Kandul, Nikolay P.; Liu, Junru; Alcantara, Lenissa; Lee, Haena; Edula, Jyotheeswara R.; Raban, Robyn; Zhan, Yinpeng; Wang, Yijin; DeBeaubien, Nicolas A.; Chan, Jieyan; Sánchez, Héctor Martínez; Bennett, Jared B.; Antoshechkin, Igor; Montell, Craig; Marshall, John M.; Akbari, Omar S.

doi:10.21203/rs.3.rs-367110/v1

Cited by 5 publications

(3 citation statements)

References 29 publications

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“…Here we provide the proof-of-concept for a next-generation TI-pgSIT technology. TI-pgSIT addresses two major limitations of the previously described pgSIT 15,16,35 . First, pgSIT relies on the separate inheritance of two required components, Cas9 endonuclease and gRNAs, that are activated in the F 1 progeny when combined by a genetic cross.…”

Section: Discussionmentioning

confidence: 99%

“…One limitation of the TI-pgSIT approach is the heatshock requirement during F 1 development, which would preclude the release of eggs. This means that the original pgSIT approach may be better suited for insects with a diapause during the egg stage 15,16 , though both the pgSIT and TI-pgSIT approaches will work well for the insects with a pupal diapause. Other than this limitation, the TI-pgSIT approach retains the benefits of the pgSIT technology, such as its noninvasiveness and high efficiency 15 .…”

Section: Discussionmentioning

confidence: 99%

“…We recently developed an alternative platform for the generation and sex separation of sterile males using the CRISPR-mediated precision guided SIT (pgSIT) technology 15,16 . This technology mechanistically relies on lethal/sterile mosaicism, mediated by the precision and accuracy of CRISPR, to simultaneously disrupt specific genes essential for female viability and male fertility during development, ensuring the exclusive production of sterile males.…”

Section: Introductionmentioning

confidence: 99%

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Temperature-Inducible Precision Guided Sterile Insect Technique

Kandul¹,

Liu²,

Akbari³

2021

Preprint

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Releases of sterile males are the gold standard for many insect population control programs, and precise sex sorting to remove females prior to male releases is essential to the success of these operations. To advance traditional methods for scaling the generation of sterile males, we previously described a CRISPR-mediated precision-guided sterile insect technique (pgSIT), in which Cas9 and gRNA strains are genetically crossed to generate sterile males for release. While effective at generating F1 sterile males, pgSIT requires a genetic cross between the two parental strains which requires maintenance and sexing of two strains in a factory. Therefore, to further advance pgSIT by removing this crossing step, here we describe a next-generation Temperature-Inducible pgSIT (TI-pgSIT) technology and demonstrate its proof-of-concept in Drosophila melanogaster. Importantly, we were able to develop a true-breeding strain for TI-pgSIT that eliminates the requirement for sex sorting, a feature that may help further automate production at scale.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Temperature-Inducible Precision Guided Sterile Insect Technique

Kandul¹,

Liu²,

Akbari³

2021

Preprint

Self Cite

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Combating mosquito-borne diseases using genetic control technologies

et al. 2021

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Mosquito-borne diseases, such as dengue and malaria, pose significant global health burdens. Unfortunately, current control methods based on insecticides and environmental maintenance have fallen short of eliminating the disease burden. Scalable, deployable, genetic-based solutions are sought to reduce the transmission risk of these diseases. Pathogen-blocking Wolbachia bacteria, or genome engineering-based mosquito control strategies including gene drives have been developed to address these problems, both requiring the release of modified mosquitoes into the environment. Here, we review the latest developments, notable similarities, and critical distinctions between these promising technologies and discuss their future applications for mosquito-borne disease control.

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Genetically Encoded CRISPR components Yield Efficient Gene Editing in the Invasive Pest,Drosophila suzukii

Kandul¹,

Belikoff

Liu³

et al. 2021

Preprint

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Originally from Asia, Drosophila suzukii (Matsumura, 1931, Diptera: Drosophilidae) is presently a global pest of economically important soft-skinned fruits. Also commonly known as spotted wing Drosophila (SWD), it is largely controlled through repeated applications of broad-spectrum insecticides. There is a pressing need for a better understanding of SWD biology and for developing alternative environmentally-friendly methods of control. The RNA-guided Cas9 nuclease has revolutionized functional genomics and is an integral component of several recently developed genetic strategies for population control of insects. Here we have developed transgenic strains that encode three different terminators and four different promoters to express Cas9 in both the soma and/or germline of SWD. The Cas9 lines were evaluated through genetic crossing to transgenic lines that encode single guide RNAs targeting the conserved X-linked yellow body and white eye genes. We find that several Cas9/gRNA lines display very high editing capacity. Going forward, these tools will be instrumental for evaluating gene function in SWD and may provide tools useful for the development of new genetic strategies for control of this invasive species.

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Eliminating Mosquitoes with Precision Guided Sterile Males

Cited by 5 publications

References 29 publications

Temperature-Inducible Precision Guided Sterile Insect Technique

Temperature-Inducible Precision Guided Sterile Insect Technique

Combating mosquito-borne diseases using genetic control technologies

Genetically Encoded CRISPR components Yield Efficient Gene Editing in the Invasive Pest,Drosophila suzukii

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