ELISA and dot-blot detection of flavescence dorée-MLO in individual leafhopper vectors during latency and inoculative state

Boudon‐Padieu, E.; Larrue, J.; Caudwell, A.

doi:10.1007/bf01570882

Cited by 65 publications

(42 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the last 15 years, some progress has been made towards enabling the enrichment of phytoplasmal fractions from extracts of infected plants and insects (4,20,21,42,47,48). In some cases, it became possible to produce phytoplasma-specific polyclonal and monoclonal antibodies, greatly facilitating diagnosis (6,9). However, as detailed below, only a few phytoplasmal genes have been characterized.…”

mentioning

confidence: 99%

Identification and Characterization of Phytoplasmal Genes, Employing a Novel Method of Isolating Phytoplasmal Genomic DNA

et al. 2003

View full text Add to dashboard Cite

Phytoplasmas are unculturable, insect-transmissible plant pathogens belonging to the class Mollicutes. To be transmitted, the phytoplasmas replicate in the insect body and are delivered to the insect's salivary glands, from where they are injected into the recipient plant. Because phytoplasmas cannot be cultured, any attempt to recover phytoplasmal DNA from infected plants or insects has resulted in preparations with a large background of host DNA. Thus, studies of the phytoplasmal genome have been greatly hampered, and aside from the rRNA genes, only a few genes have hitherto been isolated and characterized. We developed a unique method to obtain host-free phytoplasmal genomic DNA from the insect vector's saliva, and we demonstrated the feasibility of this method by isolating and characterizing 78 new putative phytoplasmal open reading frames and their deduced proteins. Based on the newly accumulated information on phytoplasmal genes, preliminary characteristics of the phytoplasmal genome are discussed.

show abstract

mentioning

confidence: 99%

Identification and Characterization of Phytoplasmal Genes, Employing a Novel Method of Isolating Phytoplasmal Genomic DNA

et al. 2003

View full text Add to dashboard Cite

show abstract

“…A Flavescence dorée (FD) fitoplazma és a Scaphoideus titanus kabó-cafaj esetében ezt a különös helyzetet azzal magyarázzák (Bressan et al 2005), hogy a vektorrovar és a fitoplazma co-evolúciója csak nagyon rövid idôre nyúlik vissza, hiszen 2000, Santini és Lucchi 1998). A fitoplazmát a rovar nyálmirigyébôl szerológiai módszer-rel (ELISA) és molekuláris módszerrel (PCR) is sikerült kimutatni, valamint az átviteli kísérletek is az átvitel lehetôségét bizonyítják (Boudon-Padieu et al 1989, Carraro et al 1994, Mori et al 2002.…”

Section: Magyarországi Helyzetunclassified

Risk of a spreading a dangerous new grapevine disease, Flavescence dorée (FD) phytoplasma, and the distribution of its leafhopper vector (Scaphoideus titanus Ball) in Hungary

Benedek¹

2014

Acta Agronomica Óváriensis

View full text Add to dashboard Cite

“…FD-infected leafhoppers were obtained by feeding young adults on FD-diseased V. faba for 3 weeks (feeding access period), then on healthy Zea mays L. Infected leafhoppers were used after 8 weeks following the beginning of feeding access, when the phytoplasma titer is high in their body (Boudon-Padieu et al, 1989). Other phytoplasmas were maintained in periwinkle.…”

Section: Phytoplasma Sourcesmentioning

confidence: 99%

“…were individually crushed with a glass rod in a final volume of500pl PBS (phosphate-bufferedsaline: 137 mM NaC1, 2.7 mM KC1, 8 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.4) (Boudon-Padieu et al, 1989). The supernatant of a 2,900 g x 5 min centrifugation was used for ELISA (100 #1 per well).…”

Section: Preparation Of Antigens For Elisa Leafhoppers Healthy and Fmentioning

confidence: 99%

“…Antisera to FD-phytoplasma have been produced (Boudon-Padieu et al, 1989) but thorough cross absorption was needed to get rid of antibodies to host components. However, western blots with polyclonal antibodies raised to phytoplasma-enriched fractions of FD-infected broadbean have demonstrated the presence in FD-infected leafhoppers of two main and 7-10 minor components which were not detected in healthy leafhopper control (Boudon-Padieu et al, 1987;Seddas et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Generation and characterization of monoclonal antibodies to Flavescence dorée phytoplasma: Serological relationships and differences in electroblot immunoassay profiles of Flavescence dorée and elm yellows phytoplasmas

et al. 1996

View full text Add to dashboard Cite

Eleven stable hybridoma cell lines secreting monoclonal antibodies specific for FD-phytoplasma, the pathogenic agent of grapevine Flavescence dor6e, were produced by fusing a non-secreting myeloma cell line with spleen cells from Balb/c mice immunized with Flavescence dor6e phytoplasma purified by immunoaffinity. These monoclonal antibodies were characterized for their recognition of phytoplasma proteins by western blot. Six of eleven reacted specifically in ELISA and immunoblotting with Elm-yellows phytoplasma. These antibodies did not react either in ELISA or in western blot with preparations from periwinkles infected with phytoplasmas that cause GYU (Grapevine Yellows from Udine), AP (Apple Proliferation), EAY (European Aster Yellows) and Stoic (Stolbur from France). Two of these hybridoma lines were used routinely for the immunodiagnosis of Flavescence dor6e phytoplasma in diseased grapevines.Abbreviations: ELISA -Enzyme linked immunosorbent assay; FD -Flavescence dor6e; MLO -Mycoplasmalike organism; EY -Elm yellows.

show abstract

ELISA and dot-blot detection of flavescence dorée-MLO in individual leafhopper vectors during latency and inoculative state

Cited by 65 publications

References 21 publications

Identification and Characterization of Phytoplasmal Genes, Employing a Novel Method of Isolating Phytoplasmal Genomic DNA

Identification and Characterization of Phytoplasmal Genes, Employing a Novel Method of Isolating Phytoplasmal Genomic DNA

Risk of a spreading a dangerous new grapevine disease, Flavescence dorée (FD) phytoplasma, and the distribution of its leafhopper vector (Scaphoideus titanus Ball) in Hungary

Generation and characterization of monoclonal antibodies to Flavescence dorée phytoplasma: Serological relationships and differences in electroblot immunoassay profiles of Flavescence dorée and elm yellows phytoplasmas

Contact Info

Product

Resources

About