Ellagic acid induces a dose- and time-dependent depolarization of mitochondria and activation of caspase-9 and -3 in human neuroblastoma cells

Alfredsson, Christina Fjæraa; Ding, Menglei; Liang, Qiwei; Sundström, Birgitta; Nånberg, Eewa

doi:10.1016/j.biopha.2013.08.010

Cited by 25 publications

(17 citation statements)

References 30 publications

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“…This assay is regularly used to show the senescence statues in vitro conditions. 46,47 The activity of these caspases reduced significantly in H 2 O 2 -treated rat embryonic fibroblast cells after exposure to EA. 40 In accordance with literature, H 2 O 2 effectively enhanced the LPO level and ROS production in NIH3T3 cells, whereas exposure to the hydroalcoholic extracts of all test samples; F. vulgaris, I. tataricum, A. chamaecistus, and S. flavida depleted both ROS and LPO levels, respectively, as compared with the control group.…”

Section: Discussionmentioning

confidence: 98%

Manipulation of molecular pathways and senescence hallmarks by natural compounds in fibroblast cells

Momtaz

Baeeri

Rahimifard

et al. 2018

J of Cellular Biochemistry

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Aging contributes to an increased risk of developing a number of neurodegenerative and chronic disorders, predominantly related to oxidative stress (OS) and defects in the antioxidant balance. This study focused on the antisenescence effect of four plant species (Falcaria vulgaris, Ixiolirion tataricum, Ajuga chamaecistus, and Scabiosa flavida) on H2O2‐induced premature senescence in rat NIH3T3 fibroblasts, which were found to be rich in effective phytochemicals with traditional ethnobotanical backgrounds. Plant materials were collected, identified, and extracted. To determine the viability of NIH3T3 cells, an MTT assay was conducted. The levels of OS markers and the senescence‐associated ß‐galactosidase (SA‐ß‐GAL) activity were analyzed by the Elisa reader. The cell cycle pattern was evaluated by flow cytometry. The expression of senescence‐related inflammatory cytokines and the molecules involved in aging signaling pathways were investigated using the real‐time reverse transcription polymerase chain reaction (RT‐PCR). H2O2 treatment decreased cell viability and increased lipid peroxidation (LPO) and the reactive oxygen species (ROS) in NIH3T3s. However, S. flavida exhibited low cytotoxicity, reduced OS and SA‐ß‐GAL activities in NIH3T3 cells compared with the H2O2‐treated group. I. tataricum was the second best plant, although it was more toxic to NIHT3T cells. S. flavida decreased G0/G1 arrest and facilitated the G2/M transition of NIH3T3s, also downregulated the expression of p38, p53, p16, and the related inflammatory mediators. S. flavida potentially modulated senescence‐associated hallmarks in fibroblasts exposed to H2O2, thus it may inhibit the aging process via controlling the OS. Therefore it is a promising candidate for future antiaging explorations.

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Section: Discussionmentioning

confidence: 98%

Manipulation of molecular pathways and senescence hallmarks by natural compounds in fibroblast cells

Momtaz

Baeeri

Rahimifard

et al. 2018

J of Cellular Biochemistry

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“…Here, it should be highlighted the dual role of EA. A number of studies show that EA induces apoptosis through the mitochondria‐dependent signalling pathways . Therefore, it can be supposed that EA in a hormesis effect can either inhibit or increase mitochondrial injury and apoptosis in a doses‐dependent way.…”

Section: Discussionmentioning

confidence: 99%

Ellagic acid confers protection against gentamicin-induced oxidative damage, mitochondrial dysfunction and apoptosis-related nephrotoxicity

Sepand

Ghahremani

Razavi-Azarkhiavi

et al. 2016

Journal of Pharmacy and Pharmacology

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“…reduction of the number of human neuroblastoma SH-SY5Y cells by alterations of the mitochondrial membrane potential, activation of Casp-3, Casp-9, fragmentation of DNA, and dose- and time-dependent cell apoptosis by the mitochondrial pathway [102,103];…”

Section: Figurementioning

confidence: 99%

The Neuroprotective Effects of Phenolic Acids: Molecular Mechanism of Action

2017

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The neuroprotective role of phenolic acids from food has previously been reported by many authors. In this review, the role of phenolic acids in ameliorating depression, ischemia/reperfusion injury, neuroinflammation, apoptosis, glutamate-induced toxicity, epilepsy, imbalance after traumatic brain injury, hyperinsulinemia-induced memory impairment, hearing and vision disturbances, Parkinson’s disease, Huntington’s disease, anti-amyotrophic lateral sclerosis, Chagas disease and other less distributed diseases is discussed. This review covers the in vitro, ex vivo and in vivo studies concerning the prevention and treatment of neurological disorders (on the biochemical and gene expression levels) by phenolic acids.

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Ellagic acid induces a dose- and time-dependent depolarization of mitochondria and activation of caspase-9 and -3 in human neuroblastoma cells

Cited by 25 publications

References 30 publications

Manipulation of molecular pathways and senescence hallmarks by natural compounds in fibroblast cells

Manipulation of molecular pathways and senescence hallmarks by natural compounds in fibroblast cells

Ellagic acid confers protection against gentamicin-induced oxidative damage, mitochondrial dysfunction and apoptosis-related nephrotoxicity

The Neuroprotective Effects of Phenolic Acids: Molecular Mechanism of Action

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