Elucidation of the gas vesicle gene clusters in cyanobacteria of the genus<i>Arthrospira</i>(Oscillatoriales, Cyanophyta) and correlation with ITS phylogeny

Miklaszewska, Magdalena; Waleron, Małgorzata; Morin, Nicolas; Całusińska, Magdalena; Wilmotte, Annick; Marsac, Nicole Tandeau de; Rippka, Rosemarie; Waleron, Krzysztof

doi:10.1080/09670262.2012.692817

Cited by 16 publications

(9 citation statements)

References 39 publications

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“…PLM2.Bin9 (Figure 3). Furthermore, the optimal pH conditions derived from publications, media recipes, or the isolate geography establishes the Limnospira clade with an alkaline preference (pH 8-10), as described by Nowicka-Krawczyk (ATCC medium 1679); (BioSample: SAMN10237416; Vonshak, 1997;Şeker et al, 2008;Carrieri et al, 2011;Cheevadhanarak et al, 2012;Miklaszewska et al, 2012;Lefort et al, 2014;Dong et al, 2015;Shiraishi, 2015;Dineshkumar et al, 2016;Xu et al, 2016;Suzuki et al, 2019;Yadav et al, 2020;Zhao et al, 2020). With A. platensis Paraca and Arthrospira sp.…”

Section: Discussionmentioning

confidence: 94%

De-novo Assembly of Limnospira fusiformis Using Ultra-Long Reads

et al. 2021

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The Limnospira genus is a recently established clade that is economically important due to its worldwide use in biotechnology and agriculture. This genus includes organisms that were reclassified from Arthrospira, which are commercially marketed as “Spirulina.” Limnospira are photoautotrophic organisms that are widely used for research in nutrition, medicine, bioremediation, and biomanufacturing. Despite its widespread use, there is no closed genome for the Limnospira genus, and no reference genome for the type strain, Limnospira fusiformis. In this work, the L. fusiformis genome was sequenced using Oxford Nanopore Technologies MinION and assembled using only ultra-long reads (>35 kb). This assembly was polished with Illumina MiSeq reads sourced from an axenic L. fusiformis culture; axenicity was verified via microscopy and rDNA analysis. Ultra-long read sequencing resulted in a 6.42 Mb closed genome assembled as a single contig with no plasmid. Phylogenetic analysis placed L. fusiformis in the Limnospira clade; some Arthrospira were also placed in this clade, suggesting a misclassification of these strains. This work provides a fully closed and accurate reference genome for the economically important type strain, L. fusiformis. We also present a rapid axenicity method to isolate L. fusiformis. These contributions enable future biotechnological development of L. fusiformis by way of genetic engineering.

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Section: Discussionmentioning

confidence: 94%

De-novo Assembly of Limnospira fusiformis Using Ultra-Long Reads

et al. 2021

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“…Cyanobacteria developed various defense mechanisms to protect themselves. First, the cells may avoid the stress-inducing or damaging factor by changing the buoyancy of the trichome or its rotation (Miklaszewska et al, 2012 ). Secondly, cells may repair the damaged DNA or synthesize DNA de novo (Sinha and Häder, 2002 ).…”

Section: Cultivation Methodsmentioning

confidence: 99%

“…The most characteristic feature of GvpC protein is the presence of a highly conserved motif of 33 amino acid residues, forming tandem repeats. Miklaszewska et al ( 2012 ) have proposed the following structure for the gvp operon: gvpA1-gvpC1-gvpA2-gvpC2-gvpA3-gvpC3-gvpN , which was identical in five analyzed Arthrospira strains. Although the operon could not be assembled during genome analysis, its structure could be determined by gene cloning.…”

Section: Geneticsmentioning

confidence: 96%

Edible Cyanobacterial Genus Arthrospira: Actual State of the Art in Cultivation Methods, Genetics, and Application in Medicine

et al. 2017

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The cyanobacterial genus Arthrospira appears very conserved and has been divided into five main genetic clusters on the basis of molecular taxonomy markers. Genetic studies of seven Arthrospira strains, including genome sequencing, have enabled a better understanding of those photosynthetic prokaryotes. Even though genetic manipulations have not yet been performed with success, many genomic and proteomic features such as stress adaptation, nitrogen fixation, or biofuel production have been characterized. Many of above-mentioned studies aimed to optimize the cultivation conditions. Factors like the light intensity and quality, the nitrogen source, or different modes of growth (auto-, hetero-, or mixotrophic) have been studied in detail. The scaling-up of the biomass production using photobioreactors, either closed or open, was also investigated to increase the production of useful compounds. The richness of nutrients contained in the genus Arthrospira can be used for promising applications in the biomedical domain. Ingredients such as the calcium spirulan, immulina, C-phycocyanin, and γ-linolenic acid (GLA) show a strong biological activity. Recently, its use in the fight against cancer cells was documented in many publications. The health-promoting action of “Spirulina” has been demonstrated in the case of cardiovascular diseases and age-related conditions. Some compounds also have potent immunomodulatory properties, promoting the growth of beneficial gut microflora, acting as antimicrobial and antiviral. Products derived from Arthrospira were shown to successfully replace biomaterial scaffolds in regenerative medicine. Supplementation with the cyanobacterium also improves the health of livestock and quality of the products of animal origin. They were also used in cosmetic preparations.

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“…O9.13F is regulated by gas vesicles ( Figure 2 F) encoded by certain identified genes: gvpA , gvpC , gvpK , gvpFL , gvpV , gvpW , gvpN and gvpJ . GvpA proteins form the vesicle core, while GvpC proteins provide structural support and influence the shape of the gas vesicles [ 92 ]. The proteins GvpK, GvpFL and GvpN possibly stabilise the structure of the gas vesicle.…”

Section: Resultsmentioning

confidence: 99%

“…The GvpJ protein is considered to determine the shape of the gas vesicle. The functions of the GvpV and GvpW proteins remain unknown [ 92 , 93 ].…”

Section: Resultsmentioning

confidence: 99%

Comparative Genomics and Physiological Investigation of a New Arthrospira/Limnospira Strain O9.13F Isolated from an Alkaline, Winter Freezing, Siberian Lake

Misztak

Waleron

Furmaniak

et al. 2021

Cells

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Cyanobacteria from the genus Arthrospira/Limnospira are considered haloalkalotolerant organisms with optimal growth temperatures around 35 °C. They are most abundant in soda lakes in tropical and subtropical regions. Here, we report the comprehensive genome-based characterisation and physiological investigation of the new strain O9.13F that was isolated in a temperate climate zone from the winter freezing Solenoye Lake in Western Siberia. Based on genomic analyses, the Siberian strain belongs to the Arthrospira/Limnospira genus. The described strain O9.13F showed the highest relative growth index upon cultivation at 20 °C, lower than the temperature 35 °C reported as optimal for the Arthrospira/Limnospira strains. We assessed the composition of fatty acids, proteins and photosynthetic pigments in the biomass of strain O9.13F grown at different temperatures, showing its potential suitability for cultivation in a temperate climate zone. We observed a decrease of gamma-linolenic acid favouring palmitic acid in the case of strain O9.13F compared to tropical strains. Comparative genomics showed no unique genes had been found for the Siberian strain related to its tolerance to low temperatures. In addition, this strain does not possess a different set of genes associated with the salinity stress response from those typically found in tropical strains. We confirmed the absence of plasmids and functional prophage sequences. The genome consists of a 4.94 Mbp with a GC% of 44.47% and 5355 encoded proteins. The Arthrospira/Limnospira strain O9.13F presented in this work is the first representative of a new clade III based on the 16S rRNA gene, for which a genomic sequence is available in public databases (PKGD00000000).

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Elucidation of the gas vesicle gene clusters in cyanobacteria of the genusArthrospira(Oscillatoriales, Cyanophyta) and correlation with ITS phylogeny

Cited by 16 publications

References 39 publications

De-novo Assembly of Limnospira fusiformis Using Ultra-Long Reads

De-novo Assembly of Limnospira fusiformis Using Ultra-Long Reads

Edible Cyanobacterial Genus Arthrospira: Actual State of the Art in Cultivation Methods, Genetics, and Application in Medicine

Comparative Genomics and Physiological Investigation of a New Arthrospira/Limnospira Strain O9.13F Isolated from an Alkaline, Winter Freezing, Siberian Lake

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