Em14-3-3 delivered by PLGA and chitosan nanoparticles conferred improved protection in chicken against Eimeria maxima

Haseeb, Muhammad Tahir; Huang, Jin‐Shun; Lakho, Shakeel Ahmed; Zhang, Yang; Hasan, Muhammad Waqqas; Ehsan, Muhammad; Aleem, Muhammad Tahir; Memon, Muhammad Ali; Ali, Haider; Song, Xiaoling; Ren, Yan; Xu, Lixin; Li, Xiangrui

doi:10.1007/s00436-021-07420-4

Cited by 8 publications

(5 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Followed by the similar procedures, the EE and LC reached 82.40 ± 0.06% and 2.00 ± 0.01% respectively in PLGA-rEtTA4 nanospheres ( 40 ). Reported in another paper, the EE was 89.35 ± 1.18% in PLGA-rEm14-3-3 nanospheres, and in CS-rEm14-3-3 nanospheres reaches 83.46% ( 61 ). However, by using similar procedures with minor modifications, the EE reached 55% in the formulation of CS-PLGA-rOmp22 nanospheres, while the LC was about 0.94% ( 62 ).…”

Section: Discussionmentioning

confidence: 83%

Actin depolymerizing factor-based nanomaterials: A novel strategy to enhance E. mitis-specific immunity

et al. 2022

Front. Immunol.

Self Cite

View full text Add to dashboard Cite

The epidemic of avian coccidiosis seriously threatens the animals’ welfare and the economic gains of the poultry industry. Widespread in avian coccidiosis, Eimeria mitis (E. mitis) could obviously impair the production performance of the infected chickens. So far, few effective vaccines targeting E. mitis have been reported, and the nanovaccines composed of nanospheres captured our particular attention. At the present study, we construct two kinds of nanospheres carrying the recombinant E. mitis actin depolymerizing factor (rEmADF), then the characterization was then analyzed. After safety evaluation, the protective efficacy of rEmADF along with its nanospheres were investigated in chickens. The promoted secretions of antibodies and cytokines, as well as the enhanced percentages of CD4+ and CD8+ T cells were evaluated by the ELISA and flow cytometry assay. In addition, the absolute quantitative real-time PCR (qPCR) assay implied that vaccinations with rEmADF-entrapped nanospheres could significantly reduce the replications of E. mitis in feces. Compared with the rEmADF-loaded chitosan (EmADF-CS) nanospheres, the PLGA nanospheres carrying rEmADF (EmADF-PLGA nanosphers) were more effective in up-regulating weight efficiency of animals and generated equally ability in controlling E. mitis burdens in feces, suggesting the PLGA and CS nanospheres loaded with rEmADF were the satisfactory nanovaccines for E. mitis defense. Collectively, nanomaterials may be an effective antigen delivery system that could help recombinant E. mitis actin depolymerizing factor to enhance immunoprotections in chicken against the infections of E. mitis.

show abstract

Section: Discussionmentioning

confidence: 83%

Actin depolymerizing factor-based nanomaterials: A novel strategy to enhance E. mitis-specific immunity

et al. 2022

Front. Immunol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…It was noteworthy that the EE and LC of formulated nanospheres varied in previous studies. The EE of PLGA-rEm14-3-3 nanospheres reached 89.35% ± 1.18%, while the EE of CS-rEm14-3-3 nanospheres was 83.46% ± 1.57% ( 56 ). With similar procedures, the EE of PLGA-rEtTA4 nanospheres reached 82.40% ± 0.06%, while the LC of PLGA-rEtTA4 nanospheres was 2.00% ± 0.01% ( 46 ).…”

Section: Discussionmentioning

confidence: 99%

PLGA Nanospheres as Delivery Platforms for Eimeria mitis 1a Protein: A Novel Strategy to Improve Specific Immunity

et al. 2022

Front. Immunol.

Self Cite

View full text Add to dashboard Cite

The infections of chicken coccidiosis impact the welfare of chickens and the economical production of poultry. Eimeria mitis is ubiquitous in chicken coccidiosis, and E. mitis infection can significantly affect the productivity of birds. Up to now, few efficient vaccines against E. mitis have been reported, whereas the recombinant subunit vaccines delivered by nanomaterials may elicit an encouraging outcome. Thus, in this study, we chose E. mitis 1a (Em1a) protein as the candidate antigen to generate Em1a preparations. The recombinant Em1a (rEm1a) protein was encapsulated with poly lactic-co-glycolic acid (PLGA) and chitosan (CS) nanospheres. The physical characterization of the rEm1a-PLGA and rEm1a-CS nanospheres was investigated, and the resulting nanospheres were proven to be nontoxic. The protective efficacy of rEm1a-PLGA and rEm1a-CS preparations was evaluated in E. mitis-challenged birds in comparison with two preparations containing rEm1a antigen emulsified in commercially available adjuvants. ELISA assay, flow cytometry analysis, and quantitative real-time PCR (qPCR) analysis indicated that vaccination with rEm1a-loaded nanospheres significantly upregulated the secretions of antibodies and cytokines and proportions of CD4+ and CD8+ T lymphocytes. Compared with the other three preparations, rEm1a-PLGA nanosphere was more effective in improving growth performance and inhibiting oocyst output in feces, indicating that the PLGA nanosphere was associated with optimal protection against E. mitis. Collectively, our results highlighted the advantages of nanovaccine in eliciting protective immunity and may provide a new perspective for developing effective vaccines against chicken coccidiosis.

show abstract

“…Concurrently, CD8þ cells increase in the periphery after infection with various Eimeria spp., while the CD3þ and CD4þ cell responses vary (40–42). Recombinant coccidia antigen can increase CD8þ cells in vivo (30,43).…”

Section: Coccidiosismentioning

confidence: 99%

“…Various adjuvants have been tested for their ability to improve the immune answer after parenteral vaccination against coccidia. The most frequently used ones are interleukin (IL) 2, sometimes in combination with interferon gamma (93–96), but other cytokines including IL 15, IL 17, and IL 18 (80,97,98) as well as a variety of nanoparticles (43,74,99,100) have been used with some success.…”

Section: Coccidiosismentioning

confidence: 99%

Vaccination Against Poultry Parasites

Hauck,

Macklin

2023

Avian Diseases

View full text Add to dashboard Cite

The complexity of parasites and their life cycles makes vaccination against parasitic diseases challenging. This review highlights this by discussing vaccination against four relevant parasites of poultry. Coccidia, i.e., Eimeria spp., are the most important parasites in poultry production, causing multiple billions of dollars of damage worldwide. Due to the trend of antibiotic-free broiler production, use of anticoccidia vaccines in broilers is becoming much more important. As of now, only live vaccines are on the market, almost all of which must be produced in birds. In addition, these live vaccines require extra care in the management of flocks to provide adequate protection and prevent the vaccines from causing damage. Considerable efforts to develop recombinant vaccines and related work to understand the immune response against coccidia have not yet resulted in an alternative. Leucozytozoon caulleryi is a blood parasite that is prevalent in East and South Asia. It is the only poultry parasite for which a recombinant vaccine has been developed and brought to market. Histomonas meleagridis causes typhlohepatitis in chickens and turkeys. The systemic immune response after intramuscular vaccination with inactivated parasites is not protective. The parasite can be grown and attenuated in vitro, but only together with bacteria. This and the necessary intracloacal application make the use of live vaccines difficult. So far, there have been no attempts to develop a recombinant vaccine against H. meleagridis. Inactivated vaccines inducing antibodies against the poultry red mite Dermanyssus gallinae have the potential to control infestations with this parasite. Potential antigens for recombinant vaccines have been identified, but the use of wholemite extracts yields superior results. In conclusion, while every parasite is unique, development of vaccines against them shares common problems, namely the difficulties of propagating them in vitro and the identification of protective antigens that might be used in recombinant vaccines.RESUMEN. Estudio recapitulativo-Vacunación contra los parásitos de las aves de corral. La complejidad de los parásitos y sus ciclos de vida hace que la vacunación contra las enfermedades parasitarias sea un desafı o. Esta revisión destaca este concepto al discutir la vacunación contra cuatro parásitos relevantes en la avicultura. Las coccidias, como, Eimeria spp., son los parásitos más importantes en la producción avı cola y causan daños por miles de millones de dólares en todo el mundo. Debido a la tendencia de la producción de pollos de engorde sin antibióticos, el uso de vacunas anticoccidianas en pollos de engorde se está volviendo mucho más importante. Por el momento, sólo hay en el mercado vacunas vivas y casi todas ellas deben producirse en aves. Además, estas vacunas vivas requieren un cuidado especial en el manejo de las parvadas para brindar una protección adecuada y evitar que las vacunas causen daños. Los esfuerzos considerables para desarrollar vacunas recombinantes y los trabajos...

show abstract

Em14-3-3 delivered by PLGA and chitosan nanoparticles conferred improved protection in chicken against Eimeria maxima

Cited by 8 publications

References 55 publications

Actin depolymerizing factor-based nanomaterials: A novel strategy to enhance E. mitis-specific immunity

Actin depolymerizing factor-based nanomaterials: A novel strategy to enhance E. mitis-specific immunity

PLGA Nanospheres as Delivery Platforms for Eimeria mitis 1a Protein: A Novel Strategy to Improve Specific Immunity

Vaccination Against Poultry Parasites

Contact Info

Product

Resources

About