Embracing the era of antimicrobial peptides with marine organisms

Chen, Pengyu; Ye, Ting; Li, Chunyuan; Praveen, Praveen; Hu, Zhangli; Li, Wenyi; Shang, Chenjing

doi:10.1039/d3np00031a

Cited by 20 publications

(9 citation statements)

References 152 publications

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“…Despite the potential of marine bacteria to produce bacteriocins, only a few marine bacteriocins have been identified [ 12 , 33 ]. This could be due to the laborious and time-consuming conventional isolation process.…”

Section: Discussionmentioning

confidence: 99%

“…To date, the in silico prediction of bacteriocins gene clusters has been performed within bacteria found in the human gastrointestinal tract [ 4 ], rumen [ 7 , 8 ] and artisanal cheese [ 9 ], as well as within the bacterial genus Geobacillus [ 3 ]. Although both terrestrial and aquatic bacteriocins could present a promising alternative to antibiotics [ 10 ], very few studies related to the exploration of marine bacteriocins have been carried out [ 11 , 12 ]. A bacteriocin, BaCf3, produced by the marine strain Bacillus amyloliquefaciens was found to be thermostable and active against an antibiotic-resistant strain, Bacillus circulans , through an in vitro study [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…The marine habit is known to be an untapped reservoir of new and unique natural products, including antimicrobial peptides. This is attributed to the uniqueness of the organisms producing them and to the extremophilic biodiversity in the marine environment [ 12 , 14 ]. Among the bacterial phyla widely distributed in the marine ecosystem, Bacillota (formerly named as Firmicutes ) is recognized for its potential antibacterial activity [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

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In Silico Screening of Bacteriocin Gene Clusters within a Set of Marine Bacillota Genomes

Teber,

Asakawa

2024

IJMS

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Due to their potential application as an alternative to antibiotics, bacteriocins, which are ribosomally synthesized antimicrobial peptides produced by bacteria, have received much attention in recent years. To identify bacteriocins within marine bacteria, most of the studies employed a culture-based method, which is more time-consuming than the in silico approach. For that, the aim of this study was to identify potential bacteriocin gene clusters and their potential producers in 51 marine Bacillota (formerly Firmicutes) genomes, using BAGEL4, a bacteriocin genome mining tool. As a result, we found out that a majority of selected Bacillota (60.78%) are potential bacteriocin producers, and we identified 77 bacteriocin gene clusters, most of which belong to class I bacteriocins known as RiPPs (ribosomally synthesized and post-translationally modified peptides). The identified putative bacteriocin gene clusters are an attractive target for further in vitro research, such as the production of bacteriocins using a heterologous expression system.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In Silico Screening of Bacteriocin Gene Clusters within a Set of Marine Bacillota Genomes

Teber,

Asakawa

2024

IJMS

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“…Among them, antimicrobial peptides are of interest because of low ability to induce drug resistance. 13,14 In general, most of marine antimicrobial peptides are isolated from animals, 15,16 and marine microorganisms are also a hot spot for screening novel antimicrobial peptides. 17,18 In our present study, a Bacillus licheniformis (B. licheniformis) M1 was isolated from the sediment of a deep-sea cold spring, which exhibited obvious antimicrobial activities against foodborne pathogens.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Novel Antimicrobial Peptide Bacipeptin against Foodborne Pathogens

Wei,

Hu,

Sun

et al. 2024

J. Agric. Food Chem.

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The increasing emergence of multidrug-resistant pathogens and development of biopreservatives in food industries has increased the demand of novel and safe antimicrobial agents. In this study, a marine bacterial strain Bacillus licheniformis M1 was isolated and exhibited obvious antimicrobial activities against foodborne pathogens, especially against methicillin-resistant Staphylococcus aureus. The antimicrobial agent was purified and identified as a novel antimicrobial peptide, which was designated as bacipeptin, and the corresponding mechanism was further investigated by electron microscopy observation and transcriptomic analysis with biochemical validation. The results showed that bacipeptin could reduce the virulence of methicillin-resistant Staphylococcus aureus and exerted its antimicrobial activity by interfering with histidine metabolism, inducing the accumulation of reactive oxygen species and down-regulating genes related to Na + /H + antiporter and the cell wall, thus causing damage to the cell wall and membrane. Overall, our study provides a novel natural product against foodborne pathogens and discloses the corresponding action mechanism.

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“…Anti-microbial peptides (AMPs), also called host defense peptides (HDPs), are innate immune response molecules known for their broad-spectrum activity against various classes of microorganisms [4,5]. They can exert their activity by directly killing microorganisms or by carrying out actions that stimulate the host's immune responses [5,6]. Among the microbicidal mechanisms of action of AMPs are membrane disruption, prevention of cell wall formation, and inhibition of protein synthesis and folding [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Aquiluscidin, a Cathelicidin from Crotalus aquilus, and the Vcn-23 Derivative Peptide, Have Anti-Microbial Activity against Gram-Negative and Gram-Positive Bacteria

Hernández-Arvizu,

Silis-Moreno,

García-Arredondo

et al. 2023

Microorganisms

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Anti-microbial peptides play a vital role in the defense mechanisms of various organisms performing functions that range from the elimination of microorganisms, through diverse mechanisms, to the modulation of the immune response, providing protection to the host. Among these peptides, cathelicidins, a well-studied family of anti-microbial peptides, are found in various animal species, including reptiles. Due to the rise in anti-microbial resistance, these compounds have been suggested as potential candidates for developing new drugs. In this study, we identified and characterized a cathelicidin-like peptide called Aquiluscidin (Aq-CATH) from transcripts obtained from the skin and oral mucosa of the Querétaro’s dark rattlesnake, Crotalus aquilus. The cDNA was cloned, sequenced, and yielded a 566-base-pair sequence. Using bioinformatics, we predicted that the peptide precursor contains a signal peptide, a 101-amino-acid conserved cathelin domain, an anionic region, and a 34-amino-acid mature peptide in the C-terminal region. Aq-CATH and a derived 23-amino-acid peptide (Vcn-23) were synthesized, and their anti-microbial activity was evaluated against various species of bacteria in in vitro assays. The minimal inhibitory concentrations against bacteria ranged from 2 to 8 μg/mL for both peptides. Furthermore, at concentrations of up to 50 μM, they exhibited no significant hemolytic activity (<2.3% and <1.2% for Aquiluscidin and Vcn-23, respectively) against rat erythrocytes and displayed no significant cytotoxic activity at low concentrations (>65% cell viability at 25 µM). Finally, this study represents the first identification of an antimicrobial peptide in Crotalus aquilus, which belongs to the cathelicidin family and exhibits the characteristic features of these peptides. Both Aq-CATH and its derived molecule, Vcn-23, displayed remarkable inhibitory activity against all tested bacteria, highlighting their potential as promising candidates for further antimicrobial research.

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Embracing the era of antimicrobial peptides with marine organisms

Abstract: The marine world is a rich reservoir for the discovery of antimicrobial peptides, namely antibiotic alternatives. The current review summarized the most recently identified AMPs, with a focus on exploiting the PTMs for mature AMP production.

Cited by 20 publications

References 152 publications

In Silico Screening of Bacteriocin Gene Clusters within a Set of Marine Bacillota Genomes

In Silico Screening of Bacteriocin Gene Clusters within a Set of Marine Bacillota Genomes

Characterization of a Novel Antimicrobial Peptide Bacipeptin against Foodborne Pathogens

Aquiluscidin, a Cathelicidin from Crotalus aquilus, and the Vcn-23 Derivative Peptide, Have Anti-Microbial Activity against Gram-Negative and Gram-Positive Bacteria

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