2009
DOI: 10.1007/s12522-009-0010-4
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Embryo development after intracytoplasmic sperm injection can be predicted by assessment of sperm nuclear chromatin

Abstract: Purpose To assess the influence of structural differences in sperm nuclei on embryo development in intracytoplasmic sperm injection (ICSI). Methods Semen obtained from forty-four infertile patients who underwent ICSI was examined. In assessing blastocyst development, only those patients who had successfully obtained over five fertilized eggs were included to exclude any oocyte factors (n = 22). Spermatozoa were assessed using excitation fluorescence flow cytometry with acridine orange and the sperm chromatin d… Show more

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References 42 publications
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