Embryo fragmentation as a determinant of blastocyst development in vitro and pregnancy outcomes following embryo transfer

Stone, Bronte A.; Greene, J. S.; Vargyas, Joyce M.; Ringler, Guy E.; Marrs, Richard P.

doi:10.1016/j.ajog.2005.02.048

Cited by 34 publications

(28 citation statements)

References 12 publications

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“…This effect of embryo fragmentation is independent of maternal age and may be the most important index of continued embryo viability in extended culture media. These findings are in line with that of previous investigators that the morphologic grade of day 3 embryos is a strong indicator directly correlated with blastocyst formation (6,7). It has been suggested that the degree of fragmentation in cleavage-stage embryos is directly proportional to the incidence of chromosomal abnormality, thereby reducing the capacity of the embryo to reach blastocyst (10)(11)(12)(13).…”

supporting

confidence: 91%

Age does not influence the effect of embryo fragmentation on successful blastocyst development

Reynolds

Maxwell

et al. 2011

Fertility and Sterility

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supporting

confidence: 91%

Age does not influence the effect of embryo fragmentation on successful blastocyst development

Reynolds

Maxwell

et al. 2011

Fertility and Sterility

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“…This finding confirms the value of early cleavage as a strong indicator, not only for blastocyst formation but also for its quality (Lundin et al, 2001;neuber et al, 2003;Van Montfoort et al, 2004;Giorgetti et al, 2007), which appears to have a very close relation with implantation rates and the success of blastocyst transfer (Balaban et al, 2000). Embryo morphology at day three has been reported to be a good predictor for the chances of an embryo to achieve the blastocyst stage (Rijnders & Jansen 1998;Stone et al, 2005;nomura et al, 2007). The analysis of data in this study demonstrates that the probability of grade 1 and 2a embryos to get to blastocyst stage is 48% (Table 3), which is significantly higher than the chances of embryos with grade 3 or 4 (3.2%).…”

Section: Resultssupporting

confidence: 70%

“…Coincidently, Graham (Graham et al, 2000) reported that only 48% embryos that would have been chosen for embryo transfer and/or cryopreservation on day 3 were eventually used in such a manner at the blastocyst stage . Previous reports showed that dysmorphic embryos on day three have higher rates of aneuploidy, polyploidy and mosaicism (Munne et al, 1995) and reduces the chances of an embryo to develop until day five, although, some types of fragmentation are transient and do not diminish the likelihood of blastocyst formation (Stone et al, 2005). nevertheless, blastocysts more commonly have a normal karyotype than cleavage-stage embryos do (Jansen et al, 2008) and this has to be considered relevant when preimplantation genetic diagnosis is not available, because an extended in vitro culture until day five could be a good alternative to select the best embryos to be transferred.…”

Section: Resultsmentioning

confidence: 99%

“…Many parameters have been used to predict the outcome of cleaving embryos, such as the speed of cleavage (Luna et al, 2008), number of blastomeres (Shapiro et al, 2000;Langley et al, 2001;Luna et al, 2008) and embryo morphology at day 3 (Rijnders & Jansen 1998;Stone et al, 2005;nomura et al, 2007), all of which, provide relevant elements to evaluate the capacity of cleaving embryos to reach blastocyst. This study combines all parameters, allowing a comparison between them, and also provides novel information about the characteristics of developing embryos when blastocysts have 100% implantation.…”

Section: Discussionmentioning

confidence: 99%

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Embryo early cleavage, number of blastomeres and morphology at day three as factors to predict blastocyst development

Mackenna¹,

Crosby²,

Zegers‐Hochschild³

2013

JBRA Assisted Reproduction

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Copyright -Todos os direitos reservados a SBRA -Sociedade Brasileira de Reprodução AssistidaEmbryo early cleavage, number of blastomeres and morphology at day three as factors to predict blastocyst development Clivagem precoce do embrião, número de blastocistos e morfologia embrionária no dia 3 como fatores preditivos para o desenvolvimento até blastocisto ABSTRACTObjective: the objective of this study is to evaluate markers of embryo quality, applied on day three of in vitro culture, in their capacity to predict whether embryos will reach blastocyst stage. Methods: a retrospective analysis of embryology database coming from 2772 zygotes obtained in 564 oocyte retrievals was performed; embryos were observed from day one to five: 23 to 26 hours after insemination all zygotes were evaluated to assess early cleavage, the number of blastomeres and embryo morphology were registered on day three and blastocyst formation was evaluated on day five; early first cleavage, number of blastomeres and embryo morphology at day three were correlated with embryo development until day five. Results: blastocyst formation rate was 36% in embryos with early cleavage and 19% in embryos without early the probability of a good quality embryo (grade 1 and 2a) at day three to reach the blastocyst stage was 48%, which was significantly higher than the chances of lower quality embryos. Conclusion: factors reported in this study -early cleavage, number of blastomeres and embryo morphology -are key words: blastocyst, expanded blastocyst, early cleavage, blastomere number, embryo morphology. RESUMENObjetivo: el objetivo del presente trabajo es evaluar los marcadores de calidad embrionaria, observados el tercer día de cultivo, para predecir el desarrollo del embrión hasta blastocisto. Métodos: se realizó un análisis retrospectivo de la base de datos del Laboratorio de Embriología, que proviene de 2772 cigotos obtenidos de 564 aspiraciones foliculares; los embriones fueron evaluados desde el día uno al quinto de desarrollo, registrando lo siguiente, 23 a 26 horas después de la inseminación, todos los cigotos fueron evaluados para determinar el clivaje temprano; el día 3 de desarrollo se evaluó el número de blastómeros y morfología embrionaria; el día quinto se evaluó la formación de un blastocisto, se correlacionó el clivaje temprano, el número de blastomeros y la morfología embrionariaa con el desarrollo embrionario hasta el día quinto. Resultados: la tasa de blastulación fue 36% en embriones con clivaje temprano y 19% en los embriones sin -día 3, la probabilidad de los embriones de buena calidad (grado 1 y 2a) en día 3 de desarrollo de alcanzar el estadío de blastocisto fue de 48%, que fue significativamente mejor que la probabilidad de los embriones de peor morfología. Conclusión: los factores reportados en este estudioclivaje temprano, número de blastomeros y morfología embrionaria -son útiles para decidir el cultivo prolongado in vitro hasta día quinto de desarrollo embrionario.

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“…However, the hCG, which reflects the activity of cells from the trophoblast lineage, declines within a few days thereafter, followed by menstrual period, indicating loss of pregnancy. The incidence of cell death in the human blastocyst has additionally been shown to be directly correlated with early embryo quality (Hardy et al, 1989;Stone et al, 2005), and deregulated cell death very likely contributes to low developmental potential of human embryos.…”

Section: Cell Death Prior To Implantationmentioning

confidence: 98%

Molecular mechanisms of trophoblast survival: From implantation to birth

2005

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Fetal development depends upon a coordinated series of events in both the embryo and in the supporting placenta. The initial event in placentation is appropriate lineage allocation of stem cells followed by the formation of a spheroidal trophoblastic shell surrounding the embryo, facilitating implantation into the uterine stroma and exclusion of oxygenated maternal blood. In mammals, cellular proliferation, differentiation, and death accompany early placental development. Programmed cell death is a critical driving force behind organ sculpturing and eliminating abnormal, misplaced, nonfunctional, or harmful cells in the embryo proper, although very little is known about its physiological function during placental development. This review summarizes current knowledge of the cell death patterns and molecular pathways governing the survival of cells within the blastocyst, with a focus on the trophoblast lineage prior to and after implantation. Particular emphasis is given to human placental development in the context of normal and pathological conditions. As molecular pathways in humans are poorly elucidated, we have also included an overview of pertinent genetic animal models displaying defects in trophoblast survival.

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Embryo fragmentation as a determinant of blastocyst development in vitro and pregnancy outcomes following embryo transfer

Cited by 34 publications

References 12 publications

Age does not influence the effect of embryo fragmentation on successful blastocyst development

Age does not influence the effect of embryo fragmentation on successful blastocyst development

Embryo early cleavage, number of blastomeres and morphology at day three as factors to predict blastocyst development

Molecular mechanisms of trophoblast survival: From implantation to birth

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