Embryo rescue: A potential tool for improvement of economically important crops

Krishna, Ram; Khandagale, Kiran; Benke, Ashwini Prashant; Soumia, P. S.; Manjunathagowda, Dalasanuru Chandregowda; Ansari, Waquar Akhter; Mokat, Digmbar N.; Gawande, Suresh Janardan; Ade, Avinash B.; Singh, Major

doi:10.1016/b978-0-323-90795-8.00009-6

Cited by 5 publications

(8 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Notably, younger embryos may present more challenges than differentiated ones, with later-stage embryos, particularly during the autotrophic phase, exhibiting higher success rates [76]. Additionally, studies have shown that torpedo-stage embryos boast the highest success rates [16].…”

Section: Other Factors Influencing Embryo Rescuementioning

confidence: 99%

“…It involves recovering embryos from seeds that fail to germinate or develop, followed by in vitro culture on a nutrient medium to facilitate their growth [11][12][13]. This method not only aids in retrieving valuable genetic material but also allows the production of hybrids with unattainable desired traits through traditional breeding [12,[14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

“…Plants 2024, 13, x FOR PEER REVIEW 2 of 14 method not only aids in retrieving valuable genetic material but also allows the production of hybrids with unattainable desired traits through traditional breeding [12,[14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Widening Genetic Diversity Using Embryo Rescue in Cucurbit Crops: A Review

Reddy,

Ramireddy,

Reddy

2024

Plants

View full text Add to dashboard Cite

Embryo rescue is a vital technique in cucurbit breeding and propagation, addressing challenges such as embryo abortion, poor seed viability, and incompatibility barriers. This method involves the excision of immature embryos from seeds followed by their in vitro culture on a nutrient medium, providing an environment conducive to their growth and development. In cucurbits, embryo rescue has been extensively utilized to overcome barriers to hybridization, enabling the production of interspecific and intergeneric hybrids with desired traits. Various factors, including genotype, developmental stage of embryos, and culture conditions, influence the success of embryo rescue in cucurbits. Optimal nutrient formulations, growth regulators, and culture techniques are critical for promoting embryo germination, shoot elongation, and subsequent plantlet establishment. Additionally, embryo rescue facilitates the recovery of valuable genetic material from wild and exotic cucurbit species, expanding genetic diversity and developing novel cultivars with improved traits such as disease resistance, yield, and quality. This review highlights the principles, applications, and advancements in embryo rescue technology in cucurbits, emphasizing its significance in cucurbit breeding programs and crop improvement efforts.

show abstract

Section: Other Factors Influencing Embryo Rescuementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Widening Genetic Diversity Using Embryo Rescue in Cucurbit Crops: A Review

Reddy,

Ramireddy,

Reddy

2024

Plants

View full text Add to dashboard Cite

show abstract

“…Embryo rescue (ER) is a set of techniques commonly used to rescue immature/maturelethal embryos, and hybrid embryos generated from interspecific and intergeneric crosses unable to survive in vivo or during traditional plant breeding practices [11][12][13][14][15][16][17][18][19]. The procedure involves excising immature or lethal embryos and culturing them in vitro on a specific nutrient culture medium.…”

Section: Introductionmentioning

confidence: 99%

“…ER has been instrumental in recovering haploid, dihaploid, doubled haploid (DH), polyploid embryos, addition lines, and substitution lines resulting from interspecific or intergeneric crosses. ER techniques have been also used to shorten the reproductive cycle, propagate rare plants, study the physiology of seed germination, understand embryo nutrition, and study embryo morphogenesis from the zygote to cotyledon stage [12,17,19,[25][26][27][28][29][30][31] (Figure 1). This review provides up-to-date information on ER techniques, their impact, and applications in the genetic improvement in major and minor crops.…”

Section: Introductionmentioning

confidence: 99%

Embryo Rescue in Plant Breeding

Rogo,

Fambrini,

Pugliesi

2023

Plants

View full text Add to dashboard Cite

Embryo rescue (ER) techniques are among the oldest and most successful in vitro tissue culture protocols used with plant species. ER refers to a series of methods that promote the development of an immature or lethal embryo into a viable plant. Intraspecific, interspecific, or intergeneric crosses allow the introgression of important alleles of agricultural interest from wild species, such as resistance or tolerance to abiotic and biotic stresses or morphological traits in crops. However, pre-zygotic and post-zygotic reproductive barriers often present challenges in achieving successful hybridization. Pre-zygotic barriers manifest as incompatibility reactions that hinder pollen germination, pollen tube growth, or penetration into the ovule occurring in various tissues, such as the stigma, style, or ovary. To overcome these barriers, several strategies are employed, including cut-style or graft-on-style techniques, the utilization of mixed pollen from distinct species, placenta pollination, and in vitro ovule pollination. On the other hand, post-zygotic barriers act at different tissues and stages ranging from early embryo development to the subsequent growth and reproduction of the offspring. Many crosses among different genera result in embryo abortion due to the failure of endosperm development. In such cases, ER techniques are needed to rescue these hybrids. ER holds great promise for not only facilitating successful crosses but also for obtaining haploids, doubled haploids, and manipulating the ploidy levels for chromosome engineering by monosomic and disomic addition as well substitution lines. Furthermore, ER can be used to shorten the reproductive cycle and for the propagation of rare plants. Additionally, it has been repeatedly used to study the stages of embryonic development, especially in embryo-lethal mutants. The most widely used ER procedure is the culture of immature embryos taken and placed directly on culture media. In certain cases, the in vitro culture of ovule, ovaries or placentas enables the successful development of young embryos from the zygote stage to maturity.

show abstract