Embryo technologies in the horse

Squires, E.L.; Carnevale, E.M.; McCue, Patrick M.; Bruemmer, J.E.

doi:10.1016/s0093-691x(02)01268-2

Cited by 139 publications

(127 citation statements)

References 53 publications

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“…OPU entails puncture of ovarian follicles. In sheep and goats, laparoscopy combined with OPU is a popular method (Pieterse et al, 1991;Carter et al, 2000;Galli et al, 2001;Abeydeera, 2002;Baldassarre et al, 2002;Cogine et al, 2003Cogine et al, , 2004Squires et al, 2003;Colleoni et al, 2007;Paramio, 2010).…”

Section: In Vitro Maturation Of Oocytes (Ivm)mentioning

confidence: 99%

“…The most frequently used methods of obtaining material from slaughterhouse samples are: puncture of an ovarian follicle with a needle connected to a suction pump (in all species), sectioning of ovaries (mostly in mares), and isolation of ovarian follicles and subsequent collection of oocytes (in all species) (Abeydeera, 2002;Cogine et al, 2003;Squires et al, 2003).…”

Section: In Vitro Maturation Of Oocytes (Ivm)mentioning

confidence: 99%

“…In mares, oocytes can be surrounded by both tightly packed and dispersed layers of the corona radiata, but both types of oocytes are competed for maturation (Squires et al, 2003;Betteridge, 2006;Hinrichs, 2010Hinrichs, , 2010a.…”

Section: In Vitro Maturation Of Oocytes (Ivm)mentioning

confidence: 99%

“…All of these requirements are characteristic of the respective farm animal species and have been described in detail in the literature: cattle (Hoshi, 2003;Lonergan, 2007;Lonergan and Fair, 2008), pigs (Abeydeera, 2002;Nagai et al, 2006;Lonergan and Fair, 2008;Gil et al, 2010;Dang-Nguyen et al, 2011), sheep and goats (Cognie et al, 2003;Paramio, 2010), and horses (Squires at el., 2003;Betteridge, 2006Betteridge, , 2007Hinrichs, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Obtaining farm animal embryos <i>in vitro</i>

Duszewska¹,

Rapala²,

Trzeciak³

et al. 2012

J. Anim. Feed Sci.

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This paper reviews the basic knowledge about obtaining farm animal embryos in vitro with special focus on differences among species and application of this procedure in the future. In vitro production of farm animal embryos consists of in vitro maturation (IVM) of oocytes, in vitro fertilization (IVF) of matured oocytes, and in vitro culture (IVC) of embryos. Oocytes can be collected from live animals (by laparotomy, laparoscopy, Ovum Pick Up) or from slaughtered ones (by puncture, sectioning). Usually immature oocytes are isolated, and during IVM they reach maturity. Matured oocytes are cultured with sperm (IVF), leading to the formation of zygotes. In the case of fertilization problems (horse, pig), intracytoplasmic sperm injection is used. The zygotes are usually cultured (IVC) to the morula and blastocyst stages. These embryos can be transferred to recipients or frozen/vitrified. Offspring have been obtained after transfer of cattle, sheep, goat, pig and horse embryos. This procedure can be used in animal breeding, biotechnology, medicine, and basic research.

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Section: In Vitro Maturation Of Oocytes (Ivm)mentioning

confidence: 99%

Section: In Vitro Maturation Of Oocytes (Ivm)mentioning

confidence: 99%

Section: In Vitro Maturation Of Oocytes (Ivm)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Obtaining farm animal embryos <i>in vitro</i>

Duszewska¹,

Rapala²,

Trzeciak³

et al. 2012

J. Anim. Feed Sci.

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show abstract

“…However, techniques that involve the manipulation of oocytes and/or embryos may themselves be detrimental to embryo viability and subsequent development (ALLEN, 2005;COUTINHO DA SILVA, 2008;SQUIRES et al, 2003). Quality of the equine embryo has a major effect on pregnancy rates, and embryo cryopreservation (ALLEN, 2005;LAGARES et al, 2009;SQUIRES, 2005).…”

Section: Introductionmentioning

confidence: 99%

Informação estrutural de lipídios a partir de um único embrião equino pela técnica de MALDI-MS

Gonçalves

Ferreira

Orlandi

et al. 2014

Braz. J. Vet. Res. Anim. Sci.

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It was reported the potential of MALDI-MS for the characterization of lipid species present in a single equine embryo, and studied some lipid structures detected by collision induced dissociation (CID) experiments. In the positive ion mode spectrum, it were observed mostly protonated and sodiated species of sphingomyelins (SM), phosphatidylcholines (PC) and triacylglycerols (TAG). In the negative ion mode, it were observed phosphatidylethanolamines (PE) and phosphatidylinositols (PI). MS/MS spectrum of most intense lipid ions was performed to show MALDI-MS/MS structural information potential.

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Embryo Transfer andin vitroFertilization

Hasler¹

2007

Comparative Reproductive Biology

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Embryo technologies in the horse

Cited by 139 publications

References 53 publications

Obtaining farm animal embryos <i>in vitro</i>

Obtaining farm animal embryos <i>in vitro</i>

Informação estrutural de lipídios a partir de um único embrião equino pela técnica de MALDI-MS

Embryo Transfer andin vitroFertilization

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