Embryo Transfer (Techniques, Donors, and Recipients)

Phillips, Patrick E.; Jahnke, Marianna M.

doi:10.1016/j.cvfa.2016.01.008

Cited by 27 publications

(33 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In recent times, considerable advancement has been made in controlling ovarian function and biochemistry of gonadotropins; however, the inherent factors affecting superovulation outcomes in donor animals are still partially understood ( Bó & Mapletoft, 2014 ; Mapletoft, 2018 ; Phillips & Jahnke, 2016 ; Viana et al., 2018 ). The preferred hormone for super-stimulation is FSH (follicle-stimulating hormone), as animals treated with eCG (equine chorion gonadotropin) show abnormal LH profile and declined ovulation and fertilization rates ( Murphy, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

GnRH or estradiol benzoate combination with CIDR improves in-vivo embryo production in bovines (Bos indicus and Bos taurus) under subtropics

Mahmood

Tahir

Butt

et al. 2021

PeerJ

View full text Add to dashboard Cite

Multiple Ovulation and Embryo Transfer (MOET) technology is a potential technique to upgrade livestock species’ genetics. The varied response to super-stimulatory treatments remains one of the limiting factors to this technology’s widespread use. The present study was aimed to improve the superovulation response and in-vivo embryo production by using controlled internal drug release (CIDR)-GnRH or CIDR-EB (Estradiol Benzoate) along with conventional superovulation protocol in Holstein Frisian (HF): Bos taurus; n = 42) and Crossbred (XB: Cholistani (Bos indicus) × HF; n = 28) cows. In the CIDR-GnRH/CIDR-EB treatment, CIDR was implanted in the cows after confirming the presence of a corpus luteum (CL) on the 8th day after estrus. 2 ml GnRH (Lecirelin acetate 0.0262 mg/ml) or 2 mg EB was also administered in CIDR-GnRH/CIDR-EB groups, respectively. Both groups were given super-stimulatory treatment from the 11th day after estrus (FSH in tapering doses twice a day for four consecutive days). On day 13, two doses of 2 ml prostaglandin (75 µg/ml of dextrorotatory cloprostenol) were administered (am: pm), and CIDR was removed the following day. Two artificial inseminations (AI) of the cows were performed (12 h apart) on the 15th day. No CIDR and GnRH/E.B were given in the control group, but the remaining superovulation protocol was the same. Later on, seven days after the first AI, non-surgical embryo flushing was done. The transferable embryos produced from three different superovulation protocols were then transferred into the recipient cows (n = 90) for determining their fertility. Statistical analysis revealed that the number of super-estrus follicles (SEF), multiple corpora lutea (MCL), ovulation/fertilization percentage, fertilized structures recovered (FSR), and transferable embryos (TEs) remained significantly higher (p < 0.05), and days taken for return to estrus (RTE) after embryo collection remained significantly lower (p < 0.05) in CIDR-GnRH (n = 18) and CIDR-EB (n = 15) groups as compared to the control (n = 37). The comparison between XB and HF cows revealed that the TEs production in CIDR-GnRH (XB = 5 vs HF = 13) and CIDR-EB (XB = 6 vs HF = 9) based superovulation protocols were 11.60 ± 4.08 vs 04.31 ± 0.98 and 09.33 ± 1.78 vs 05.22 ± 1.36, respectively. TEs production in XB cows (n = 5) of the CIDR-GnRH group was significantly higher (11.60 ± 4.08) than other groups. On the other hand, the days taken for RTE after embryo collection remained significantly lower (p < 0.05) in HF cows of treatment groups. However, the fertility of TEs was neither affected significantly (p > 0.05) by the superovulation protocol used nor by breed differences among donor cows. In conclusion, using CIDR-GnRH or CIDR-EB along with conventional superovulation protocol may enhance the efficiency of MOET programs in cattle. Furthermore, XB donor cows demonstrated a better performance than HF donor cows under subtropical conditions.

show abstract

Section: Introductionmentioning

confidence: 99%

GnRH or estradiol benzoate combination with CIDR improves in-vivo embryo production in bovines (Bos indicus and Bos taurus) under subtropics

Mahmood

Tahir

Butt

et al. 2021

PeerJ

View full text Add to dashboard Cite

show abstract

“…The procedures of freezing and thawing cause injuries that could compromise the viability of the embryos, especially those previously classified as of poor quality (revised by Martemucci and D'Alessandro, 2013). For these reasons, over the years in animal assisted reproduction it has been stated that only embryos of grade 1 and 2 quality could undergo freezing without significant reduction of pregnancy rates, while regular and poor quality embryos should only be transferred as fresh (Phillips and Jahnke, 2016). However, results of the present study .…”

Section: Discussionmentioning

confidence: 54%

Freezing goat embryos at different developmental stages and quality using ethylene glycol and a slow cooling rate

Fonseca

Batista

Souza-Fabjan

et al. 2018

Arq. Bras. Med. Vet. Zootec.

View full text Add to dashboard Cite

The efficiency of an alternative freezing protocol for goat embryos of different morphology and quality was tested. Fifty-eight embryos on Day 6-7 stage were transferred as fresh or after freeze-thawing (n=29/group). For freezing, embryos were placed into 1.5M ethylene-glycol solution for 10min. During this time, they were loaded in the central part of 0.25mL straw, separated by air bubble from columns containing PBS/BSA 0.4% plus 20% BFS. Straws were then frozen using a freezing machine from 20ºC to -6ºC at a cooling rate of 3ºC/min, stabilization for 15min (seeding after 5min), from -6 C to -32ºC at 0.6 C/min,and plunged into liquid nitrogen. Frozen embryos were thawed for 30s at 37ºC in a water bath. Embryos subjected to fresh transfer were maintained in holding medium (37ºC). Fresh and frozen-thawed embryos were transferred at day 7 post-estrus to 30 recipients. Kidding and kid born rates were similar (P> 0.05), respectively, for recipients receiving fresh (66.7% or 10/15; 55.2% or 16/29) or frozen-thawed (60% or 9/15; 51.7% or 15/29) embryos. The cryopreservation of goat embryos using slow-freezing protocol and 1.5MEG resulted in similar efficiency rates of fresh embryos.

show abstract

“…Embryos were classified according to their development stage, on a scale from 1 (one cell stage) to 9 (hatched blastocyst stage) and according to their quality as 1 (excellent), 2 (good), 3 (fair) and 4 (degenerate) (Phillips and Jahnke, 2016). For cryopreservation, morula and blastocyst stage embryos with qualities 1 and 2 were placed in a freezing medium with 10% ethylene glycol and 0.3 M sucrose and placed into French straws (Baruselli et al, 2015).…”

Section: Embryo Search and Evaluationmentioning

confidence: 99%

Effect of the Strategic Supplementation for Producing Embryos of Native Hartón del Valle Heifers

Riascos-Vallejos¹,

Reyes-González

Medina-Mesa³

et al. 2021

American Journal of Animal and Veterinary Sciences

View full text Add to dashboard Cite

show abstract

Embryo Transfer (Techniques, Donors, and Recipients)

Cited by 27 publications

References 13 publications

GnRH or estradiol benzoate combination with CIDR improves in-vivo embryo production in bovines (Bos indicus and Bos taurus) under subtropics

GnRH or estradiol benzoate combination with CIDR improves in-vivo embryo production in bovines (Bos indicus and Bos taurus) under subtropics

Freezing goat embryos at different developmental stages and quality using ethylene glycol and a slow cooling rate

Effect of the Strategic Supplementation for Producing Embryos of Native Hartón del Valle Heifers

Contact Info

Product

Resources

About