Embryo Transformation, A Practical Approach for Realizing Transgenic Plants of Safflower (Carthamus tinctorius L.)

Rohini, V. K.

doi:10.1006/anbo.2000.1278

Cited by 44 publications

(22 citation statements)

References 27 publications

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“…has not yet been established, globular embryos from leaf explants could be used for transformation studies. Agrobacterium-mediated transformation of embryos has been shown to be useful for difficult dicotyledonous and monocotyledonous species (Ninkovic et al 1995;Rohini and Rao 2000;Yokoi and Toriyama 1999). Therefore, it is expected that plant regeneration from globular embryos may be of great value for the genetic engineering of Scaevola.…”

Section: Histological Analysismentioning

confidence: 98%

Somatic embryogenesis from leaf explants of Australian fan flower, Scaevola aemula R. Br.

Wang

Bhalla

2004

Plant Cell Reports

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Somatic embryogenesis from leaf explants of Scaevola aemula R. Br. was achieved. Somatic embryos were induced from explants cultured on MS medium supplemented with 0.2 mg/ 2,4-dichlorophenoxyacetic acid and 0.2-0.5 mg/l 6-benzylaminopurine (BAP). Various developmental stages of somatic embryos were found on this medium-from globular embryos to germinated embryos. The transfer of globular embryos to MS medium containing 0.5 mg/l BAP resulted in a high frequency of shoot regeneration. Leaf explants cultured on MS medium containing different combinations of BAP and alpha-naphthaleneacetic acid formed adventitious shoots and roots. Histological examination confirmed the process of somatic embryogenesis. Induction of somatic embryogenesis in Scaevola provides a system for studying embryogenesis in Australian native plants and will facilitate the improvement of these plants using genetic transformation techniques.

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Section: Histological Analysismentioning

confidence: 98%

Somatic embryogenesis from leaf explants of Australian fan flower, Scaevola aemula R. Br.

Wang

Bhalla

2004

Plant Cell Reports

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“…In some cases, genetic modification in plants can occur by direct gene transfer or vector-mediated gene transfer methods. However, in safflower, genetic engineering until now is confined to vector-mediated (Agrobacterium-mediated) transformation via callus-mediated regeneration (Orlikowska et al 1995;Sankara Rao and Rohini 1999) or embryo transformation (Rohini and Sankara Rao 2000). Belide et al (2011) developed a new protocol for transformation with significant improvements in both the efficiency (about 5 %) and simplicity of implementation over existing safflower transformation protocols.…”

Section: Transgenic Approachesmentioning

confidence: 99%

“…In safflower, Agrobacterium-mediated transformation protocols have been reported (Rohini and Sankara Rao 2000). However, the protocols have not been exploited for development of transgenic with agronomically desirable traits.…”

Section: Biotechnology and Functional Genomicsmentioning

confidence: 99%

Salt and drought stresses in safflower: a review

Hussain

Lyra

Farooq

et al. 2015

Agron. Sustain. Dev.

197

130

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Safflower is one of the oldest cultivated crops, usually grown at a small scale. Safflower is grown for flowers used for coloring, flavoring foods, dyes, medicinal properties, and livestock feed. Safflower is underutilized but gaining attention due to oil yield potential and the ability to grow under high temperatures, drought, and salinity. Salinity and drought have negative effects by disrupting the ionic and osmotic equilibrium of the plant cells. The stress signal is perceived by membranes then transduced in the cell to switch on the stress responsive genes. This review discusses on stress tolerance mechanisms in safflower. Strategies are proposed for enhancing drought and salt resistance in safflower.

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“…Safflower (Carthamus tinctorius L.) is an annual, broadleaf oilseed crop of the family Asteraceae that originated in southern Asia (Kohnaward et al 2012) and adapted chiefly to dry land or irrigated cropping systems (Rohini & Sankara 2000).…”

Section: Introductionmentioning

confidence: 99%

Intercropping patterns and different farming systems affect the yield and yield components of safflower and bitter vetch

Jalilian

Najafabadi

Zardashti

2017

Journal of Plant Interactions

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This factorial field experiment was done based on a randomized complete block design in Urmia University, Iran, in 2013 and was repeated in 2014. Treatments included two farming systems (high input and organic) and different intercropping patterns that alternated bitter vetch (Vicia ervilia L.) and safflower (Carthamus tinctorius L.) with row ratios of 2:2, 3:2, 4:2 and 5:2. Sole cropping of bitter vetch and safflower was used as the control. In both years, the 2:2 intercropping pattern had biomass yield advantages compared to sole cropping and the other intercropping ratios, based on greater land equivalent ratio values. Safflower had higher relative crowding coefficients, competitive ratio (CR) and aggressivity (A) values than bitter vetch. High-input farming was more effective than the organic system in both years. Safflower was the superior competitor when grown with bitter vetch, and its productivity dominated the total biomass yields. Thus, intercropping of safflower with bitter vetch has the potential to improve performance with high land-use efficiency.ARTICLE HISTORY

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Embryo Transformation, A Practical Approach for Realizing Transgenic Plants of Safflower (Carthamus tinctorius L.)

Cited by 44 publications

References 27 publications

Somatic embryogenesis from leaf explants of Australian fan flower, Scaevola aemula R. Br.

Somatic embryogenesis from leaf explants of Australian fan flower, Scaevola aemula R. Br.

Salt and drought stresses in safflower: a review

Intercropping patterns and different farming systems affect the yield and yield components of safflower and bitter vetch

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