Embryogenic callus induction, cell suspension culture, and spectrum-effect relationship between antioxidant activity and polyphenols composition of Siraitia grosvenorii cultured cells

Liu, Zebo; Zhu, Xiaofeng; Mohsin, Ali; Yin, Zhong‐Ping; Zhuang, Yingping; Zhou, Bin; Du, Linxiao; Yin, Xuejian; Liu, Nan; Wang, Zejian; Guo, Meijin

doi:10.1016/j.indcrop.2021.114380

Cited by 10 publications

(1 citation statement)

References 34 publications

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“…Through Plackett–Burman’s design and RSM, optimal proportions of plant growth regulators for a cell suspension culture of Siraitia grosvenorii were obtained. With the optimized plant growth regulators, the obtained cell biomass and polyphenols content were 32.18% and 13.86%, respectively, more than plant growth regulators proportioning before optimization [ 49 ]. Using HPLC, we determined that the profile and levels of phenolic compounds were similar between the above-ground part of intact plants and the callus culture.…”

Section: Discussionmentioning

confidence: 99%

Optimization of Biomass Accumulation and Production of Phenolic Compounds in Callus Cultures of Rhodiola rosea L. Using Design of Experiments

Эрст

Petruk

Erst

et al. 2022

Plants

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Rhodiola rosea L. is a valuable medicinal plant with adaptogenic, neuroprotective, antitumor, cardioprotective, and antidepressant effects. In this study, design of experiments methodology was employed to analyze and optimize the interacting effects of mineral compounds (concentration of NO3− and the ratio of NH4+ to K+) and two plant growth regulators [total 6-benzylaminopurine (BAP) and α-naphthylacetic acid (NAA) concentration and the ratio of BAP to NAA] on the growth and the production of total phenolic compounds (TPCs) in R. rosea calluses. The overall effect of the model was highly significant (p < 0.0001), indicating that NH4+, K+, NO3−, BAP, and NAA significantly affected growth. The best callus growth (703%) and the highest production of TPCs (75.17 mg/g) were achieved at an NH4+/K+ ratio of 0.33 and BAP/NAA of 0.33, provided that the concentration of plant growth regulators was 30 μM and that of NO3− was ≤40 mM. According to high-performance liquid chromatography analyses of aerial parts (leaves and stems), in vitro seedlings and callus cultures of R. rosea contain no detectable rosarin, rosavin, rosin, and cinnamyl alcohol. This is the first report on the creation of an experiment for the significant improvement of biomass accumulation and TPC production in callus cultures of R. rosea.

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Section: Discussionmentioning

confidence: 99%